DNA β-Amyloid_1-42 Trimer Immunization for Alzheimer Disease in a Wild-Type Mouse Model

Intranasal inoculation with an adenovirus vaccine encoding ten repeats of Aβ3-10 reduces AD-like pathology and cognitive impairment in Tg-APPswe/PSEN1dE9 mice

“…Intranasal immunization with Ad-10 × Aβ3-10-CpG generated significantly higher titers of antibody than empty vector, but lower than the Aβ peptide, which was similar to other gene vaccine (Lambracht-Washington et al, 2009). Although the antibody titers derived from Ad-10 × Aβ3-10-CpG immunization were lower than the antibody titers derived from Aβ peptide, the induced antibodies were most likely effective because the antibodies induced by Ad-10 × Aβ3-10-CpG could bind to Aβ plaques in brains of transgenic mice as the antibodies induced by Aβ42 peptide.…”

Immunization with a new DNA vaccine for Alzheimer's disease elicited Th2 immune response in BALB/c mice by in vivo electroporation

“…Although the antibody titer elicited by the p (Aβ 3-10 ) 10 -IL-4 was lower than that of the Aβ 42 peptide group, it was much higher than the pcDNA3.1(+) group. The p(Aβ 3-10 ) 10 -IL-4 vaccine developed enough antibodies levels to be considered for use as an AD vaccine [30,31] and DNA-based vaccines can induce prolonged, endogenous antigen synthesis as they are processed within the host's own immunized cells. Moreover, the antibody isotype is mainly IgG 1 , biased to Th2, which is more efficient at clearing cerebral amyloid and indicates a low probability of inflammation.…”

Immunization Therapy for Alzheimer Disease: A Comprehensive Review of Active Immunization Strategies

“…They compared immune responses in wild type mice immunized with the DNA vaccine and those with Aβ 1-42 peptide vaccine mixed with adjuvant Quil A. The antibody responses in the DNAvaccinated mice showed a significantly higher ratio of IgG1/IgG2a than those in peptide-vaccinated mice (Lambracht-Washington et al 2009). Thus, DNA vaccine seems to induce Th2 dominant immune responses.…”