DNase activity in human seminal plasma and follicular fluid and its inhibition by follicular fluid chelating agents

Bartolomé, Javier; Romeo, Sandra Claver; Dorado-Silva, Mónica; Vega, Carlos García de la; López, Carmen Aragó; Sánchez-Martín, Pascual; Johnston, Stephen D.; Gosálvez, Jaime

doi:10.1016/j.rbmo.2021.09.015

Cited by 1 publication

(2 citation statements)

References 39 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…( c ) Bioanalyzer results of cfDNA in patients with spinal cord injury (SCI1 and SCI2) compared with the control (C). Note the presence of a 150 bp DNA fragment in SCI patients (further details in [ 52 ]).…”

Section: Presence Of Dnase Activity In the Ejaculatementioning

confidence: 99%

“…This beneficial effect of FF on sperm DNA may be related to the fact that FF inhibits the nuclease activity of seminal plasma ( Figure 1 b) [ 46 ]. This inhibitory effect is due to the fact that FF contains a molecule(s) that is capable of chelating the divalent cations necessary for the activity of nucleases not only in seminal plasma but also in the nucleases that are also present in FF itself or those directly inoculated during insemination [ 52 ]. While our experimental data suggest that seminal plasma nucleases play an important role in reproduction by protecting sperm from exogenous DNA and by facilitating sperm travel through the female reproductive tract, they could also jeopardize the integrity of fertilized eggs.…”

Section: The Role Of Follicular Fluid In Controlling Dnase Activitymentioning

confidence: 99%

See 1 more Smart Citation

Role of DNase Activity in Human Sperm DNA Fragmentation

Gosálvez,

Fernández,

Johnston

et al. 2024

Biomolecules

Self Cite

View full text Add to dashboard Cite

In this clinical era of intracytoplasmic sperm injection (ICSI), where a single spermatozoon is chosen for fertilization, the diagnostic functionality of the classical parameters typically associated with fertilization, such as sperm concentration, sperm motility, acrosome integrity, and mitochondria, is perhaps becoming less critical. In contrast, the contribution of sperm DNA quality to our understanding of the impact of male fertility within the context of ICSI is gaining increasing interest and importance. Even with respect to natural conception, high levels of sperm DNA fragmentation (SDF) in the ejaculate can adversely affect reproductive outcomes. However, the precise origin of SDF pathology in sperm cells is often ambiguous and most likely to be multifactorial. Hence, the genetic makeup of an individual, unbalanced REDOX processes, enzymatic activity, environmental and lifestyle factors, and even damage during sperm handling in the laboratory all operate in a unique and often synergistic manner to produce or induce sperm DNA damage. Surprisingly, the contribution of active enzymes as potential agents of SDF has received much less attention and, therefore, is likely to be underrated. This review highlights the roles of different enzymes related to the degradation of sperm DNA as possible effectors of DNA molecules in spermatozoa.

show abstract

Section: Presence Of Dnase Activity In the Ejaculatementioning

confidence: 99%

Section: The Role Of Follicular Fluid In Controlling Dnase Activitymentioning

confidence: 99%