1980
DOI: 10.3109/01485018008986483
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DNase II in Bull and Ram Sperm Tail and Mitochondria

Abstract: Extracts of bull and ram sperm tails were prepared by DTT and CTAB treatment. Such extracts contained deoxyribonuclease 11, degrading only native double-stranded DNA at acid pH (3.9-4.5). At least three distinguishable DNase I1 activities were found in these extracts as judged by their sensitivity to thiol compounds and various anions and cations. The deoxyribonuclease I1 activity is apparently located in or in association with the mitochondria.

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Cited by 8 publications
(5 citation statements)
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“…Thus, mtDNA was not excluded from mitochondria undergoing mitophagy. After maximal autolysosomal acidification as indicated by LTR uptake, PicoGreen fluorescence gradually disappeared, consistent with previous reports of mtDNA degradation by DNAse II of lysosomes (9,11). mtDNA has a 10-to 20-fold higher mutation rate than nuclear DNA due to the proximity of mtDNA to the ROSgenerating respiratory chain, the limited DNA repair capacity of mitochondria, and the lack of protective histones in mtDNA (45).…”
Section: C313 Mitophagy In Hepatocytessupporting
confidence: 84%
“…Thus, mtDNA was not excluded from mitochondria undergoing mitophagy. After maximal autolysosomal acidification as indicated by LTR uptake, PicoGreen fluorescence gradually disappeared, consistent with previous reports of mtDNA degradation by DNAse II of lysosomes (9,11). mtDNA has a 10-to 20-fold higher mutation rate than nuclear DNA due to the proximity of mtDNA to the ROSgenerating respiratory chain, the limited DNA repair capacity of mitochondria, and the lack of protective histones in mtDNA (45).…”
Section: C313 Mitophagy In Hepatocytessupporting
confidence: 84%
“…Similarly, in the dead sperm group the rate of transgene expression increased from 0% to 5.2% with an increase in the DNA dose from 50 to 100 ng. Three possible explanation for this phenomenon are: (1) poor chance for sperm–DNA interaction with low amounts of exogenous DNA, especially when incubated for short time periods, (2) small amounts of exogenous DNA may be at higher risk of clearance by the supposedly endonuclease activities inherited in sperm (Fisher and Bartoov, 1980; Lanes et al, 2009), and (3) seminal fluid is enriched with natural factors that strongly preclude exogenous DNA to be uptaken. Therefore, there is an additional putative risk of traces of seminal fluid remaining after the sperm processing, which may preclude the sperm–DNA interaction (Zani et al, 1995; Sperandio et al, 1996).…”
Section: Discussionmentioning
confidence: 99%
“…Semen samples were prepared for SEM observations essentially as described by Fisher & Bartoov (1980) with the following modification: Washed sperm cells,were fured with ice-cold 1 % (w/v) glutaraldehyde in phosphate buffered saline for 1 h at 4"C, then washed with phosphate buffered saline and kept at 4°C.…”
Section: Scanning Electron Microscopy (Sem)mentioning
confidence: 99%