DNAzyme-Functionalized Gold Nanoparticles for Biosensing

Xiang, Yu; Wu, Peiwen; Tan, Li; Lu, Yi

doi:10.1007/10_2013_242

Cited by 19 publications

(10 citation statements)

References 280 publications

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“…DNA-functionalized gold nanoparticles (AuNPs) (Brewer et al, 2005;Giljohann et al, 2007;Patel et al, 2011;Cutler et al, 2012;Pei et al, 2012;Xiang et al, 2013;Wang et al, 2017;Liu and Liu, 2019) have been extensively studied in terms of preparing the conjugate (Liu and Liu, 2017b), sensing (Wu et al, 2018), making materials (Tan et al, 2014;Jones et al, 2015), and cell-related applications (Giljohann et al, 2010). All the four DNA bases can strongly adsorb on gold via coordination interactions (Liu et al, 2018b), and thus even non-thiolated DNA can be attached to AuNPs (Kimura-Suda et al, 2003;Pei et al, 2012;Zhang et al, 2012a,b).…”

Section: Introductionmentioning

confidence: 99%

Attaching DNA to Gold Nanoparticles With a Protein Corona

Peng

Zhu

et al. 2020

Front. Chem.

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DNA-functionalized gold nanoparticles (AuNPs) have been widely used in directed assembly of materials, biosensors, and drug delivery. This conjugate may encounter proteins in these applications and proteins may affect not only DNA adsorption but also the function of the attached DNA. Bovine serum albumin (BSA) with many cysteine residues can strongly adsorb on AuNPs and this conjugate showed high colloidal stability against salt, acid and base. Similar protection effects were also observed with a few other common proteins including catalase, hemoglobin, glucose oxidase, and horseradish peroxidase. DNA oligonucleotides without a thiol label can hardly displace adsorbed BSA, and BSA cannot displace pre-adsorbed DNA either, indicating a strongly kinetically controlled system. Thiolated DNA can be attached at a low density on the AuNPs with a BSA corona. The BSA corona did not facilitate the hybridization of the conjugated DNA, while a smaller peptide, glutathione allowed faster hybridization. Overall, proteins increase the colloidal stability of AuNPs, and they do not perturb the gold-thiol bond in the DNA conjugate, although a large protein corona may inhibit the hybridization function of DNA.

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Section: Introductionmentioning

confidence: 99%

Attaching DNA to Gold Nanoparticles With a Protein Corona

Peng

Zhu

et al. 2020

Front. Chem.

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“…When one of these forces is weak or absent, the colloid system will destabilize and the AuNPs will aggregate, resulting in a color shift from red to blue (the shift of the surface plasmon band to a longer wavelength). This is known as interparticle plasmon coupling and has been extensively used in colorimetric biosensor development [44]. Currently, techniques such as using colloidal stabilizers and cross-linking agents have shown to be successful in manipulating the dispersal and aggregation AuNPs for colorimetric generation.…”

Section: Gold Nanoparticles As Colorimetric Signal Transducermentioning

confidence: 99%

“…In addition to thiol-DNA, unmodified single-stranded DNA (ssDNA) was found to bind AuNPs with sufficient affinity, while double-stranded DNA (dsDNA) could not [63,64,65]. By introducing DNAzymes as recognition elements into AuNP systems, a series of colorimetric biosensors has been developed for a broad range of targets [44,66]. Here, we will present an overview of the signaling strategies of DNAzyme-functionalized AuNPs.…”

Section: Gold Nanoparticles As Colorimetric Signal Transducermentioning

confidence: 99%

Integrating Deoxyribozymes into Colorimetric Sensing Platforms

Chang

Zakaria

Deng

et al. 2016

Sensors

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Biosensors are analytical devices that have found a variety of applications in medical diagnostics, food quality control, environmental monitoring and biodefense. In recent years, functional nucleic acids, such as aptamers and nucleic acid enzymes, have shown great potential in biosensor development due to their excellent ability in target recognition and catalysis. Deoxyribozymes (or DNAzymes) are single-stranded DNA molecules with catalytic activity and can be isolated to recognize a wide range of analytes through the process of in vitro selection. By using various signal transduction mechanisms, DNAzymes can be engineered into fluorescent, colorimetric, electrochemical and chemiluminescent biosensors. Among them, colorimetric sensors represent an attractive option as the signal can be easily detected by the naked eye. This reduces reliance on complex and expensive equipment. In this review, we will discuss the recent progress in the development of colorimetric biosensors that make use of DNAzymes and the prospect of employing these sensors in a range of chemical and biological applications.

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“…Nanomaterials generally range in size from 1 to 100 nm, making them similar in size to basic biological structures (Xiang et al, 2014). Based on their physical, chemical, and biocompatibility properties, nanomaterials are attractive for use in biomedical applications and show great potential in biomimetic medicine (Ning et al, 2017;Das and Noh, 2018;Hasanzadeh et al, 2018).…”

Section: Introductionmentioning

confidence: 99%

Advanced Biomimetic Nanomaterials for Non-invasive Disease Diagnosis

et al. 2021

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In modern society, the incidence of cancer, inflammatory diseases, nervous system diseases, metabolic diseases, and cardiovascular diseases is on the rise. These diseases not only cause physical and mental suffering for patients, but also place an enormous burden on society. Early, non-invasive diagnosis of these diseases can reduce the physical and mental pain of patients and social stress. There is an urgent need for advanced materials and methods for non-invasive disease marker detection, large-scale disease screening, and early diagnosis. Biomimetic medical materials are synthetic materials designed to be biocompatible or biodegradable, then developed for use in the medical industry. In recent years, with the development of nanotechnology, a variety of biomimetic medical materials with advanced properties have been introduced. Biomimetic nanomaterials have made great progress in biosensing, bioimaging, and other fields. The latest advance of biomimetic nanomaterials in disease diagnosis has attracted tremendous interest. However, the application of biomimetic nanomaterials in disease diagnosis has not been reviewed. This review particularly focuses on the potential of biomimetic nanomaterials in non-invasive disease marker detection and disease diagnosis. The first part focuses on the properties and characteristics of different kinds of advanced biomimetic nanomaterials. In the second part, the recent cutting-edge methods using biosensors and bioimaging based on biomimetic nanomaterials for non-invasive disease diagnosis are reviewed. In addition, the existing problems and future development of biomimetic nanomaterials is briefly described in the third part. The application of biomimetic nanomaterials would provide a novel and promising diagnostic method for non-invasive disease marker detection, large-scale clinical screening, and diagnosis, promoting the exploitation of devices with better detection performance and the development of global clinical public health.

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DNAzyme-Functionalized Gold Nanoparticles for Biosensing

Cited by 19 publications

References 280 publications

Attaching DNA to Gold Nanoparticles With a Protein Corona

Attaching DNA to Gold Nanoparticles With a Protein Corona

Integrating Deoxyribozymes into Colorimetric Sensing Platforms

Advanced Biomimetic Nanomaterials for Non-invasive Disease Diagnosis

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