DNS-Gehalt und Chromosomenstruktur bei Amphibien

Ullerich, Fritz-Helmut

doi:10.1007/bf00293907

Cited by 26 publications

(8 citation statements)

References 55 publications

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“…This is in contrast to the great subspeci c variation, up to nine subspecies have been described in the Iberian Peninsula whose taxonomy needs to be clari ed (Alcobendas et al, 1994;Grif ths, 1996;Alcobendas, 1997;Barbadillo et al, 1999). Previous studies found that S. salamandra had values between 49.9 and 66 pg values (Sexsmith, 1968;Ullerich, 1970;Olmo, 1973;Mancino et al, 1977;King, 1990) which are well below the amount found in our study.…”

Section: Resultscontrasting

confidence: 88%

“…Triturus helveticus was assigned a 2C value of 41 pg DNA/nucleus by Mancino et al (1977) and 49.8 pg by ow cytometry (Macgregor et al, 1990), also below the values obtained in our study ( N x D 52:67 pg). Triturus alpestris was assigned 2C values ranging from 45.3-57.0by Ullerich (1970), 47 pg by ow cytometry (Macgregor et al, 1990), 48 pg (Mancino et al, 1977) or 52 pg (Sexsmith, 1968); a range that is so wide that it includes all the values obtained by us for our Iberian samples ( N x D 54:52 pg), and for all the different species of Triturus included in this study.…”

Section: Resultsmentioning

confidence: 99%

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Determination of cellular DNA content of Iberian salamanders by flow cytometry

et al. 2000

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The DNA content per diploid nucleus (2 C-value) was determined for the eight species of Iberian salamanders by flow cytometry. All species showed high values, which are characteristic of Caudata. The species with the lowest value was Pleurodeles waltl (48.25 pg DNA/cell) and Salamandra salamandra had the highest values (70.55 pg DNA/cell). There were no statistical differences between sexes in any of the eight species and DNA content was not directly related with phylogeny. The values obtained are often higher than previously published estimates obtained with older and potentially less accurate methodologies. We discuss the possible relationship between cellular DNA content and reproductive biology. Se determinó el contenido en ADN por núcleo diploide (valor 2-C) por citometría de flujo de ocho especies de salamandras ibéricas. Todas las especies mostraron altos valores, lo que es característico de Caudata. La especie con el menor valor fue Pleurodeles waltl (48,25 pg ADN/célula) mientras Salamandra salamandra tuvo las valores más altos (70,55 pg ADN/célula). No se encontraron diferencias estadísticas entre sexos en ninguna de las 8 especies y el contenido en ADN no se relacionó directamente con la filogenia. Los valores obtenidos son, a menudo, más altos que los publicados previamente con técnicas más antigüas, y potencialmente menos precisas. Discutimos las posibles relaciones entre el contenido en ADN celular y la biología reproductiva de cada especie.

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Section: Resultscontrasting

confidence: 88%

Section: Resultsmentioning

confidence: 99%

Determination of cellular DNA content of Iberian salamanders by flow cytometry

et al. 2000

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“…Tetraploid (Borkin et al 1979), aneuploid (Yadav 1974;Uzzell et al 1975;Ogielska et al 2001b), and mosaic indi-viduals (Uzzell et al 1975;Berger and Ogielska 1994) were not detected. Our results are consistent with those of other studies, in which mostly diploids (Günther 1970(Günther , 1977Ullerich 1970;Mészáros 1973;Aleksandrovskaya 1976;Berger et al , 1986Borkin et al 1979;Ebendal 1979;Koref-Santibañez 1979;Schempp and Schmidt 1981;Tunner and Heppich 1981;Plötner and Klinkhardt 1992;Kotlik and Šçlova 1994;Mikuliek and Kotlik 2001) but also triploids (Günther 1970(Günther , 1977Berger et al , 1986Tunner 1980;Plötner and Klinkhardt 1992;Mikuliek and Kotlik 2001) were studied.…”

Section: Discussionsupporting

confidence: 92%

DNA content and genome composition of diploid and triploid water frogs belonging to the Rana esculenta complex (Amphibia, Anura)

Ogielska¹,

Kierzkowski²,

Rybacki³

2004

Can. J. Zool.

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The Central European water frog Rana esculenta L., 1758 is a natural hybrid between Rana lessonae Camerano, 1882 (LL) and Rana ridibunda Pallas, 1771 (RR). Hybrids are usually diploid (RL) or triploid (LLR or RRL). Distinguishing LL from RL, RR from RL, and LLR from RRL according to external morphology is ambiguous. In this study we checked whether the DNA content in erythrocyte nuclei measured by image cytometry is useful in determination of the taxonomic status of diploids (LL, RR, and RL) and the genome composition of triploids (LLR and RRL). For exact and direct identification of parental species, as well as for determination of genome composition in hybrids, we applied actinomycin D – 4',6-diamidino-2-phenylindole chromosome staining to metaphase plates. We analyzed 43 LL, 12 RR, and 32 RL diploids, and 37 LLR and 19 RRL triploids. All diploid hybrids had 2n = 26 chromosomes, and all triploid hybrids had 3n = 39 chromosomes. Neither aneuploid nor mosaic hybrids were detected. The expected numbers of 13 R. lessonae (L) and 13 R. ridibunda (R) chromosomes in RL hybrids were recorded in about 31% of individuals. In the rest of the sample the composition was variable, ranging from 9 to 14 R chromosomes and the corresponding number of L chromosomes. The expected composition of 26 L and 13 R chromosomes was detected in about 32% of LLR triploids, whereas in the rest of the sample the composition of chromosomes ranged from 8 to 15 R chromosomes and the corresponding number of L chromosomes. The expected numbers of 26 R and 13 L chromosomes were detected in about 26% of RRL triploids, whereas in the rest of the sample the composition of chromosomes ranged from 19 to 28 R chromosomes and the corresponding number of L chromosomes. The DNA content densitometry showed that RR and RL diploids had 9.5% and 3.8% more DNA, respectively, than LL diploids. These differences, although statistically significant, were not sufficient to unequivocally discriminate LL from RL and RR from RL. Triploids had about 50% more DNA than LL diploids (49% in LLR and 51% in RRL), but these differences were too small for unequivocal determination of their genome composition.

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“…Such chromosome organization might give us the opportunity to change the content of the repeated sequences during the change of genome size without principal changes in the chromosome morphology. The opinion that the changes in genome size result in local changes of DNA in amphibian chromosomes was advanced earlier (Ullerich 1970). Some data concerning spermatosoid histones present indirect evidence of a dissimilarity between chromosome organization in amphibians and mammals: while in amphibians histone H1 is replaced by different histories, in all mammals investigated the substitution histones have similar characteristics (Kasinsky et al 1978).…”

Section: B Hypothesis Of Amphibian Chromosome Organizationmentioning

confidence: 97%

Structural characteristics of genome organization in amphibians: Differential staining of chromosomes and DNA structure

Birstein

1982

J Mol Evol

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DNS-Gehalt und Chromosomenstruktur bei Amphibien

Cited by 26 publications

References 55 publications

Determination of cellular DNA content of Iberian salamanders by flow cytometry

Determination of cellular DNA content of Iberian salamanders by flow cytometry

DNA content and genome composition of diploid and triploid water frogs belonging to the Rana esculenta complex (Amphibia, Anura)

Structural characteristics of genome organization in amphibians: Differential staining of chromosomes and DNA structure

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