Do Acid-tolerant Cyanobacteria Exist?

Steinberg, Christoph; Schäfer, Hendrik; Beisker, Wolfgang

doi:10.1002/(sici)1521-401x(199801)26:1<13::aid-aheh13>3.3.co;2-m

Cited by 2 publications

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“…Under laboratory conditions, cyanobacteria have generally been reported to prefer neutral to slightly alkaline medium for optimum growth [1,2]. In the natural environments, however, cyanobacteria extend their distribution to pH values as low as 4 [3,4]. Brock [5] speculated that absence of cyanobacteria in extremely acidic environments may be associated with their acid-sensitive chlorophyll molecules.…”

Section: Introductionmentioning

confidence: 99%

Proteomic characterization of acid stress response inSynechocystis sp. PCC 6803

2006

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A comparative proteomic analysis using 2-DE coupled with MALDI-MS and LC-MS/MS was performed in Synechocystis sp. PCC 6803 to identify protein candidates involved in acid stress response in cyanobacteria. Comparison of soluble proteins from the cytoplasmic fraction of cells grown on media set at pH 7.5 and 5.5 using 2-DE identified four proteins, which showed significant changes in the abundance. Surprisingly, several general stress proteins, either the heat shock family proteins or chaperonins, did not show perceptible fold changes in response to acidity. Compared to the cytoplasmic proteome, the periplasmic proteome showed remarkable changes as a function of external pH. Protein expression profiling at different external pH, i.e., 9.0, 7.5, 6.0 and 5.5, allowed classifying the periplasmic proteins depending on their preferential expression patterns towards acidity or alkalinity. Among the acid- and base-induced proteins, oxalate decarboxylase and carbonic anhydrase were already known for their role in pH homeostasis. Several unknown proteins from the periplasm, that showed significant changes in response to pH, provide ideal targets for further studies in understanding pH stress response in cyanobacteria. This study also identified 14 novel proteins, hitherto unknown from the periplasmic space of Synechocystis.

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Section: Introductionmentioning

confidence: 99%

Proteomic characterization of acid stress response inSynechocystis sp. PCC 6803

2006

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“…The lack of eukaryote‐specific ion‐transporter ATPases may be the molecular basis of the unsuccessful adaptation of cyanobacteria to acidic environments (Amaral Zettler et al ., 2003). However, the presence of filamentous cyanobacteria in acid lakes (pH 2.9) in Germany (Steinberg et al ., 1998) renders doubtful this postulated lower pH limit.…”

Section: Discussionmentioning

confidence: 99%

How eukaryotic algae can adapt to the Spain's Rio Tinto: a neo‐Darwinian proposal for rapid adaptation to an extremely hostile ecosystem

et al. 2007

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Summary• Microalgae contributed 60% of the total biomass in the extremely hostile (pH 2 and metal-rich waters) environment of Rio Tinto (which is used as a model for the astrobiology of Mars). These algae are closely related to nonextreme lineages, suggesting that adaptation to Rio Tinto water (RTW) must occur rapidly.• Fitness from both the microalga Dictyosphaerium chlorelloides and the cyanobacterium Microcystis aeruginosa was inhibited when they were cultured in RTW. After further incubation for several weeks, D. chlorelloides survived, as a result of the growth of a variant that was resistant to RTW, but RTW-resistant cells did not appear in M. aeruginosa .• A Luria-Delbrück fluctuation test revealed that D. chlorelloides RTW-resistant cells arose randomly by rare spontaneous mutations before the RTW exposure (1.38 × 10 -6 mutants per cell division). The mutants with a diminished fitness are maintained in nonextreme waters as the result of a balance between new RTWresistant cells arising by mutation and RTW-resistant mutants eliminated by natural selection (equilibrium at c . 15 RTW-resistant per 10 7 wild-type cells).• Rapid adaptation of eukaryotic algae to RTW could be the result of selection of RTW-resistant mutants occurring spontaneously in nonextreme populations that arrived fortuitously at the river in the past, or in the present continuously.

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Do Acid-tolerant Cyanobacteria Exist?

Cited by 2 publications

References 0 publications

Proteomic characterization of acid stress response inSynechocystis sp. PCC 6803

Proteomic characterization of acid stress response inSynechocystis sp. PCC 6803

How eukaryotic algae can adapt to the Spain's Rio Tinto: a neo‐Darwinian proposal for rapid adaptation to an extremely hostile ecosystem

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