Do Different Proportions of Antibiotics in the CTZ Paste Interfere with the Antimicrobial Action? In Vitro Study

Abstract

Objective: To evaluate the antimicrobial action of the CTZ paste in three different proportions by diffusion in agar with the microorganisms: Enterococcus faecalis, Escherichia coli, and Candida albicans. Material and Methods: Three different proportions of antibiotics were tested: GROUP A-CTZ paste in the ratio of 33.33% chloramphenicol + 33.33% tetracycline + 33.33% zinc oxide, mixed with 2 drops of eugenol (1:1:1 ratio); GROUP B-CTZ paste in the proportion of 25% chloramphenicol + 25% tetracycline + 50% zinc oxide, mixed with 2 drops of eugenol (1: 1: 2 ratio); GROUP C-CTZ paste with 13% chloramphenicol + 13% tetracycline + 74% Zinc Oxide, mixed with 2 drops of eugenol (1:1:6 ratio); PC GROUP-Positive Control (0.12% Chlorhexidine); and NC GROUP-Negative Control (0.9% Saline solution). Data were analyzed through descriptive statistics (means and standard deviation). The one-way ANOVA and Tukey's test were used, with a significance level of 5%. Results: No statistical differences for Enterococcus faecalis between groups A, B, and C (p = 0.1986) were found. There were statistical differences for Escherichia coli between groups B and C (p = 0.029), and for Candida albicans between groups A and C (p = 0.006). Groups A, B, and C had significant differences with both Positive and Negative Controls for all the microorganisms. Conclusion: The three different ratios of CTZ paste showed antimicrobial efficacy against Enterococcus faecalis, Escherichia coli, and Candida albicans microorganisms.

Antimicrobial activity of antibiotic pastes used in pulp therapy through direct contact with a multispecies biofilm: a pilot study

“…Studies evaluating the antimicrobial activity of CTZ paste against E. faecalis demonstrated inhibition of bacterial growth. 23,24,25 Our results also showed decreased CFU count for CTZ in the selective medium for E. faecalis. Colony growth of Candida spp.…”

“…This finding is consistent with previous results evaluated by tests such as agar diffusion. 3,22,25 In addition, the present study demonstrated, for the first time, the antimicrobial activity of CTZ on multispecies biofilms by direct contact with the paste.…”

Cost analysis of endodontic treatment in primary teeth: results from a randomized clinical trial

“…There are few RCTs using the CTZ paste, but its antimicrobial capacity has been evaluated and proven in several in-vitro studies. 3,4,6,40 The great disadvantage of NIET with CTZ paste is the possible change in the color of the crown. 41 This browning is caused by the presence of tetracycline in the paste composition.…”

“…2 One of the most important aspects for successful treatment is the use of antimicrobial pastes. 3,4 Deciduous teeth have a distinct anatomy, with many accessory canals, irregular rhizolysis and great microbial diversity, making endodontic treatment problematic, 5 along with the presence of the permanent successor germ and the difficulty of managing child behavior due to the patient's young age. 2 Thus, it is essential to use obturation material with a broad spectrum of action, good diffusion, and that does not cause damage to periapical tissues and permanent teeth.…”

Perspectivas do tratamento endodôntico de dentes decíduos com o uso da pasta antibiótica CTZ: uma revisão integrativa

“…Os artigos foram realizados nos seguintes países: Brasil, Colômbia e México. Seis artigos foram estudos in vitro (Andrade, 2008;Piva;Faraco Junior;Estrela, 2008;Oliveira et al, 2019;Ern et al, 2020;Sancas et al, 2020), quatro artigos foram relatos de caso (Passos;Melo;Moreira, 2008;Moura et al, 2016;González-Núñez et al, 2010;Dias et al, 2021), avaliando apenas poucos pacientes, dois artigos foram estudos transversais (Moura et al, 2018;Sousa et al, 2020), avaliando uma grande quantidade de dentes decíduos, dois foram ensaio clínico randomizado (Luengo-Ferreira et al, 2016;Luengo-Ferreira et al 2019), com uma grande quantidade de molares decíduos avaliados, um artigo caracterizou-se como estudo retrospectivo Duarte, 2014), e um como estudo prospectivo (Carloto, 2020). O ano de publicação dos artigos variou de 2008 a 2021.…”