Do Haemostasis Activation Markers that Predict Cardiovascular Disease Exist?

Stegnar, Mojca; Vene, Nina; Božič, Mojca

doi:10.1159/000083818

Cited by 22 publications

(20 citation statements)

References 57 publications

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“…Both fitness and muscle mass differed between boys and girls and most likely explains the sex differences. Increased levels of this global marker of coagulation should, however, not be interpreted without also taking the rate of fibrinolysis into consideration [57,58]. The interpretation of the TAT results was also limited by the relatively high coefficient of variance.…”

Section: Discussionmentioning

confidence: 99%

Overfatness, stunting and physical inactivity are determinants of plasminogen activator inhibitor-1activity, fibrinogen and thrombin–antithrombin complex in African adolescents

Nienaber

Pieters

Krüger

et al. 2008

Blood Coagulation &Amp; Fibrinolysis

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We examined fibrinogen, thrombin-antithrombin complex, factor VIIIc and plasminogen activator inhibitor-1 activity in African children in order to determine haemostatic profile patterning and to identify possible subdivisions at high risk for cardiovascular disease. In a cross-sectional analysis, a convenience sample of 117 girls and 78 boys (15.6 +/- 1.35 years) in a South African township was investigated within the Physical Activity in Youth study. Haemostatic variables were investigated in the total group and subdivisions for physical activity levels, maturity (Tanner staging), sex, fat percentage and height for age. Overfatness (53.6%) coexisted with stunting (17.5%). Plasminogen activator inhibitor-1 activity differed significantly between the sexes after adjustments for fat percentage and physical activity levels. Sex explained 10% and muscle mass 1% of the variance in plasminogen activator inhibitor-1 activity. Fibrinogen was significantly higher in girls than in boys (before adjustment for fat percentage), in overfat than in lean children and in stunted than in the nonstunted children (even after adjustment for fat percentage). C-reactive protein, sex and height for age were predictors of fibrinogen. Thrombin-antithrombin complex was significantly higher in girls than in boys, but after separate adjustment for physical activity and fat percentage there were no significant differences. Fitness and muscle mass explained the variance in thrombin-antithrombin complex the best. No significant differences were seen between the groups for C-reactive protein and factor VIIIc. Overfatness, stunting and inactivity negatively influenced plasminogen activator inhibitor-1 activity, fibrinogen and thrombin-antithrombin complex possibly increased the risk for cardiovascular disease. These factors are modifiable through behavioural changes and optimal nutritional status throughout the early life.

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Section: Discussionmentioning

confidence: 99%

Overfatness, stunting and physical inactivity are determinants of plasminogen activator inhibitor-1activity, fibrinogen and thrombin–antithrombin complex in African adolescents

Nienaber

Pieters

Krüger

et al. 2008

Blood Coagulation &Amp; Fibrinolysis

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“…D‐dimer (a breakdown product of cross‐linked fibrin) has been reported to be a marker of systemic prothrombotic state in patients with cardiovascular diseases 8–15 . Meta‐analyses of prospective studies have shown that plasma levels of D‐dimer were independent predictors of increased risk of coronary heart disease, stroke, peripheral arterial disease as well as cardiovascular death 11–17 …”

Section: Discussionmentioning

confidence: 99%

“…D‐dimer has proven to be useful in the diagnosis of thrombotic disorders. Increased D‐dimer level in plasma has been reported to be a reliable marker of a systemic prothrombotic state, and its measurement might be helpful in predicting thrombotic events 11–17 . In a small series of 26 patients with SSc, Ames et al.…”

mentioning

confidence: 99%

“…Increased D-dimer level in plasma has been reported to be a reliable marker of a systemic prothrombotic state, and its measurement might be helpful in predicting thrombotic events. [11][12][13][14][15][16][17] In a small series of 26 patients with SSc, Ames et al 7 found that mean levels of D-dimer were higher in patients with SSc than controls. These data prompted us to conduct this study to: (i) evaluate plasma D-dimer concentration in patients with SSc and healthy controls; and (ii) determine a possible relationship between the presence of high plasma D-dimer concentration and clinical and biological parameters of patients with SSc.…”

mentioning

confidence: 99%

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Plasma D-dimer concentration in patients with systemic sclerosis

Marie¹,

Borg²,

Hellot

et al. 2007

Br J Dermatol

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The association between high levels of plasma D-dimer (>or=500 ng mL(-1)) and macrovascular involvement in patients with SSc is likely to be an innovative issue. We suggest that D-dimer levels may be a helpful additional test to identify patients with SSc at risk to develop thrombotic arterial complications (peripheral arterial disease, stroke and coronary event); such patients with high levels of plasma D-dimer (>or=500 ng mL(-1)) may require close monitoring of vascular parameters, including especially macrovascular impairment.

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“…In order to show that our UF-SPE protocol was as or even more efficient than previously reported works when aiming at the characterization of low abundance plasma peptides, we only present here high confidence identification of low abundant (representing ~10 −6 % of total plasma proteins, by weight) endogenous fibrinopeptides A and B (Table 5). Fibrinopeptides play a key role in haemostasis (Stegnar, 2004). Such molecules can then bring informations on cardiovascular diseases and metabolic-related disorders.…”

Section: Resultsmentioning

confidence: 99%

Endogenous Plasma Peptide Detection and Identification in the Rat by a Combination of Fractionation Methods and Mass Spectrometry

et al. 2007

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Mass spectrometry-based analyses are essential tools in the field of biomarker research. However, detection and characterization of plasma low abundance and/or low molecular weight peptides is challenged by the presence of highly abundant proteins, salts and lipids. Numerous strategies have already been tested to reduce the complexity of plasma samples. The aim of this study was to enrich the low molecular weight fraction of rat plasma. To this end, we developed and compared simple protocols based on membrane filtration, solid phase extraction, and a combination of both. As assessed by UV absorbance, an albumin depletion >99% was obtained. The multistep fractionation strategy (including reverse phase HPLC) allowed detection, in a reproducible manner (CV < 30%–35%), of more than 450 peaks below 3000 Da by MALDI-TOF/MS. A MALDI-TOF/MS-determined LOD as low as 1 fmol/μL was obtained, thus allowing nanoLC-Chip/MS/MS identification of spiked peptides representing ~10−6% of total proteins, by weight. Signal peptide recovery ranged between 5%–100% according to the spiked peptide considered. Tens of peptide sequence tags from endogenous plasma peptides were also obtained and high confidence identifications of low abundance fibrinopeptide A and B are reported here to show the efficiency of the protocol. It is concluded that the fractionation protocol presented would be of particular interest for future differential (high throughput) analyses of the plasma low molecular weight fraction.

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Do Haemostasis Activation Markers that Predict Cardiovascular Disease Exist?

Cited by 22 publications

References 57 publications

Overfatness, stunting and physical inactivity are determinants of plasminogen activator inhibitor-1activity, fibrinogen and thrombin–antithrombin complex in African adolescents

Overfatness, stunting and physical inactivity are determinants of plasminogen activator inhibitor-1activity, fibrinogen and thrombin–antithrombin complex in African adolescents

Plasma D-dimer concentration in patients with systemic sclerosis

Endogenous Plasma Peptide Detection and Identification in the Rat by a Combination of Fractionation Methods and Mass Spectrometry

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