DoAtopobium vaginae, Megasphaerasp. andLeptotrichiasp. change the local innate immune response and sialidase activity in bacterial vaginosis?

Abstract

Greater participation of A vaginae, Megasphaera sp. and Leptotrichia sp. in vaginal bacterial community did not indicate a less severe form of BV; moreover, it was associated with increased sialidase activity.

Bacterial vaginosis in pregnant adolescents: proinflammatory cytokine and bacterial sialidase profile. Cross-sectional study

“…18 In agreement with previous findings, [13][14][15][16][17][18][19][20][21][22][23][24][25][26][27][28] the present study showed that sialidase was mostly detectable in situations of abnormal vaginal flora. This finding is particularly important …”

“…13,15 In both the cytokine and the sialidase assays, samples were tested in duplicates and, if the measured concentration was above the standard curve, the samples were diluted and retested. The intra and interassay variability rates were < 11.0%.…”

“…[10][11][12] Typically, bacterial vaginosis is associated with increased cervicovaginal levels of interleukin (IL)-1 beta, but in pregnant women the levels of IL-6 and IL-8 are also elevated in the presence of bacterial vaginosis. [13][14][15][16] There is still a lack of information in the literature regarding the immune response to bacterial vaginosis in adolescents. However, it has already been demonstrated that the vaginal fluids of adolescents present altered levels of several immune mediators and show lower antimicrobial activity in vitro, in comparison with adults.…”

“…Normally, IL-8 levels do not increase in situations of bacterial vaginosis, [15][16][17][18][19][20][21][22][23][24][25] which explains the unchanged number of leukocytes in vaginal smears, in comparison with normal flora. 26 On the other hand, this scenario changes when pregnant women with bacterial vaginosis are evaluated in relation to non-pregnant women with the same floral alterations, such that significantly higher IL-8 levels are seen in pregnancy.…”

Normal flora and bacterial vaginosis in pregnancy: an overview

“…These enzymes may promote increased ascending of lower genital tract organisms and sialidase increases the probability of PTB (Cauci, 2004). Marconi et al (2013) showed that women with BV and elevated loads of Leptotrichia spp., Megasphaera spp. and A. vaginae have increased sialidase activity.…”