Do lipids influence the allergic sensitization process?

Bublin, Merima; Eiwegger, Thomas; Breiteneder, Heimo

doi:10.1016/j.jaci.2014.04.015

Cited by 130 publications

(120 citation statements)

References 77 publications

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“…A very similar phenomenon also has been reported when quantifying peach profilin, demonstrating that the use of a recombinant protein isoform as standard might overestimate the profilin content of samples (Asturias et al 1999). One explanation may be that recombinant proteins are not correctly folded as natural Pru p 3, and also lack of natural ligands, such as lipids which may affect affinity (Bublin et al 2014). This has been demonstrated in apple Mal d 3, olive tree profiling, and sunflower pollen profilin (Asturias et al 1997(Asturias et al , 1998Zuidmeer et al 2005).…”

Section: Discussionsupporting

confidence: 58%

Quantification of Peach Fruit Allergen Lipid Transfer Protein by a Double Monoclonal Antibody-based Sandwich ELISA

Zhou

Yang

et al. 2015

Food Anal. Methods

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Fruit is one of the most commonly reported food allergy sources in China, and peach lipid transfer protein (Pru p 3) has been identified as the major allergen inducing systematic symptoms. Crude allergen extracts and single component allergens have been used in food allergy diagnosis and immunotherapy. Reliable and sensitive analytical methods to quantify Pru p 3 content in fruit will help to identify lowallergenic cultivars among the abundant peach genetic resources in China. In this study, we developed a sensitive sandwich ELISA method to measure Pru p 3 in peach fruit based on two monoclonal antibodies with high sensitivity and linearity. Significant variability was observed in peach cultivars, with a much higher Pru p 3 level in peach peel than in pulp. This method will be very useful to select peach varieties with low Pru p 3 content and as a diagnostic product to detect hidden allergens in processed food.

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Section: Discussionsupporting

confidence: 58%

Quantification of Peach Fruit Allergen Lipid Transfer Protein by a Double Monoclonal Antibody-based Sandwich ELISA

Zhou

Yang

et al. 2015

Food Anal. Methods

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“…The mechanisms leading to allergic sensitization are not completely understood, and they are not only influenced by the allergen itself but also by other molecules, such as lipids, that are directly bound as ligands by the allergen or are present in the allergy source [10]. Lipids from the pollen coat are codelivered with the allergens and can modulate the immune responses of predisposed subjects by interacting with the innate immune system and iiNKT cells, and they can potentially induce NKT cell-dependent allergy disease and asthma [7,11,12].…”

Section: Discussionmentioning

confidence: 99%

Human Invariant Natural Killer T Cells Respond to Antigen-Presenting Cells Exposed to Lipids from Olea europaea Pollen

Gracia

López-Relaño²,

Revilla³

et al. 2017

Int Arch Allergy Immunol

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Background: Allergic sensitization might be influenced by the lipids present in allergens, which can be recognized by natural killer T (NKT) cells on antigen-presenting cells (APCs). The aim of this study wasto analyze the effect of olive pollen lipids in human APCs, including monocytes as well as monocyte-derived macrophages (Mϕ) and dendritic cells (DCs). Methods: Lipids were extracted from olive (Olea europaea) pollen grains. Invariant (i)NKT cells, monocytes, Mϕ, and DCs were obtained from buffy coats of healthy blood donors, and their cell phenotype was determined by flow cytometry. iNKT cytotoxicity was measured using a lactate dehydrogenase assay. Gene expression of CD1A and CD1D was performed by RT-PCR, and the production of IL-6, IL-10, IL-12, and TNF-α cytokines by monocytes, Mϕ, and DCs was measured by ELISA. Results: Our results showed that monocytes and monocyte-derived Mϕ treated with olive pollen lipids strongly activate iNKT cells. We observed several phenotypic modifications in the APCs upon exposure to pollen-derived lipids. Both Mϕ and monocytes treated with olive pollen lipids showed an increase in CD1D gene expression, whereas upregulation of cell surface CD1d protein occurred only in Mϕ. Furthermore, DCs differentiated in the presence of human serum enhance their surface CD1d expression when exposed to olive pollen lipids. Finally, olive pollen lipids were able to stimulate the production of IL-6 but downregulated the production of lipopolysaccharide- induced IL-10 by Mϕ. Conclusions: Olive pollen lipids alter the phenotype of monocytes, Mϕ, and DCs, resulting in the activation of NKT cells, which have the potential to influence allergic immune responses.

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“…Prominent allergens like Bet v 1 and Der p 2 have hydrophobic cavities that can bind different lipids. Various lipids are known to skew the immune response towards allergy in a variety of relevant cases, and this has recently been well reviewed [27]. …”

Section: Mechanisms Of Sensitizationmentioning

confidence: 99%

Contributions and Future Directions for Structural Biology in the Study of Allergens

Mueller¹

2017

Int Arch Allergy Immunol

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Allergy is defined as an inappropriate immune response to something normally considered harmless. The symptomatic immune response is driven by IgE antibodies directed against allergens. The study of allergens has contributed significantly to our understanding of allergic disease in 3 main areas. First, identifying allergens as the cause of symptoms and developing allergen standards has led to many advances in exposure assessment and patient diagnostics. Second, a biochemical understanding of allergens has suggested a number of hypotheses related to the mechanisms of allergic sensitization. And finally, studies of allergen-antibody interactions have contributed to understanding the cross-reactivity of allergens, mapping patient epitopes, and the development of hypoallergens. In this review, a few select cases are highlighted where structural biology, in particular, has contributed significantly to allergen research and provided new avenues for investigation.

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Do lipids influence the allergic sensitization process?

Cited by 130 publications

References 77 publications

Quantification of Peach Fruit Allergen Lipid Transfer Protein by a Double Monoclonal Antibody-based Sandwich ELISA

Quantification of Peach Fruit Allergen Lipid Transfer Protein by a Double Monoclonal Antibody-based Sandwich ELISA

Human Invariant Natural Killer T Cells Respond to Antigen-Presenting Cells Exposed to Lipids from Olea europaea Pollen

Contributions and Future Directions for Structural Biology in the Study of Allergens

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