Do longer sequences improve the accuracy of identification of forensically important Calliphoridae species?

Bortolini, Sara; Giordani, G.; Tuccia, Fabiola; Vanin, Stefano

doi:10.7717/peerj.5962

Cited by 12 publications

(8 citation statements)

References 54 publications

(71 reference statements)

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“…Low Na concentration in soil is considered beneficial for the growth and development of many plants [ 52 ]. Plants have different salt tolerance levels, and high concentration of Na in the soil can result in low productivity of crops and also lead to sodium dispersion making agricultural soils unsuitable for growing crops [ 30 ]. If the concentration of Na in an agricultural soil is greater than 2 cmol c kg −1 , then it is on the very high level.…”

Section: Discussionmentioning

confidence: 99%

Impact of Small-Scale Mining Activities on Physicochemical Properties of Soils in Dunkwa East Municipality of Ghana

Essandoh

Takase

Bryant

2021

The Scientific World Journal

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The quality of soils in rehabilitated small-scale mined sites needs thorough investigation since a lot of changes do occur. The study assessed the impacts of small-scale mining activities on concentration and distribution of soil physicochemical properties and heavy metals. The soil samples were collected from 120 (50 m × 50 m) plots. The concentrations of soil physicochemical properties (Ca, Mg, Na, N, P, K, and OC and EC) varied significantly ( p < 0.05) between unmined and mined soils. However, there were no statistically, significant differences ( p < 0.05) observed in the concentrations of Cd, Hg, Pb, As, and Cu between the unmined and mined soils. Despite the generally poor (33.8%) soil quality in the study area, mining activities further reduced it by 24.2%. Soils from mined sites with unfilled/partially filled pits had higher levels of K, Mg, and Na. As mined sites fallow period increased, concentrations of OC and Cd increased, while Ca, Mg, pH, Cu, Pb, and As and value of EC decreased. The number of years that mined land remained fallow, and whether the pits were filled or unfilled during this period should be factored into the mined land rehabilitation processes.

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Section: Discussionmentioning

confidence: 99%

Impact of Small-Scale Mining Activities on Physicochemical Properties of Soils in Dunkwa East Municipality of Ghana

Essandoh

Takase

Bryant

2021

The Scientific World Journal

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“…Molecular approaches have been developed to meet this scientist necessity. Gene database, because of the lack of information or the incorrectness of the uploaded information, do not allow yet an identification of all the taxa recognised of forensic interest (Bortolini et al 2018;Garg et al 2019). Therefore, the support of the morphological identification approach remains uncontested.…”

Section: Discussionmentioning

confidence: 99%

“…The amplification was carried out in the mitochondrial COI barcoding region using the universal primer designed by Folmer et al (1994) with a temperature of annealing of 49.8°C as reported in Bortolini et al (2018).…”

Section: Methodsmentioning

confidence: 99%

Description of the puparium and other notes on the morphological and molecular identification of Phthitia empirica (Diptera, Sphaeroceridae) collected from animal carcasses

Giordani

Vanin

2020

Egypt J Forensic Sci

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Background: A correct identification of species is fundamental in order to derive all the biological, ecological, and behavioral information useful to reconstruct previous events when insects are used in forensic and funerary archaeological contexts. Little attention was paid in the past to the description of the immature stages of flies, especially of species belonging to family found only occasionally on human cadavers and animal carrion. Sphaeroceridae species belong to this category. Results: In this work, adult and puparia of Phthitia empirica (Hutton, 1901) are illustrated and described. In addition, some observations about the molecular identification of this species are presented and discussed. Conclusions: This work will allow the identification of puparia of this species providing additional tools in forensic entomological investigation. More in general, the effort of producing morphological descriptions and molecular information of species of potential forensic interest clearly implements the possibility to provide a deeper understanding of the peri-and post-mortem events in violent crimes.

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“…Sequencing reactions were carried out by Eurofins Operon MWG-Ebersberg, Germany. The obtained FASTA sequences were compared with GenBank DNA sequences using BLAST-n® (Altschul, Gish, Miller, Myers, & Lipman, 1990) and with home-produced sequences as reported in Bortolini, Giordani, Tuccia, Maistrello, and Vanin (2018).…”

Section: Insect Collection and Analysesmentioning

confidence: 99%

Experimental evaluation on the applicability of necrobiome analysis in forensic veterinary science

et al. 2019

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Despite the wide usage of animals as models in forensic studies, the investigations of fundamental legal questions involving domesticated and nondomesticated animals were always given marginal attention compared to “human forensic,” and only recently the interest in the discipline is increasing. Our research focuses on the effect of the fur coat on the activity and development of microbial decomposers. In order to test this variable never assessed before, rabbit carcasses were used and results show that: (i) distinct and significant temporal changes in terms of metabolic activity and taxa distribution can be tracked over the decomposition process; (ii) the richness and the diversity of the bacterial communities does not significantly vary over time, but it does not mean that the species Operational Taxonomic Units (OTUs) do not change; (iii) the presence/absence of the fur on the carcasses does not significantly affect either the bacterial communities’ functional activity or the diversity intra‐ and intercommunity, neither at phylum nor at family resolution; (iv) the functional activity and the ecological diversity of the bacterial communities are significantly affected by the body region, while the relative abundance is not. Obtained data confirm previous observations and provide new insight in the Forensic Veterinary field in terms of equally using them in order to derive a statistical model for the PMI estimation. As a future perspective, a contribution to the Forensic Entomology approach will be given in legal investigations when domestic or wild animals are involved, regardless of the presence of a hair layer.

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Do longer sequences improve the accuracy of identification of forensically important Calliphoridae species?

Cited by 12 publications

References 54 publications

Impact of Small-Scale Mining Activities on Physicochemical Properties of Soils in Dunkwa East Municipality of Ghana

Impact of Small-Scale Mining Activities on Physicochemical Properties of Soils in Dunkwa East Municipality of Ghana

Description of the puparium and other notes on the morphological and molecular identification of Phthitia empirica (Diptera, Sphaeroceridae) collected from animal carcasses

Experimental evaluation on the applicability of necrobiome analysis in forensic veterinary science

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