Do mismatched genitalia promote mechanical isolation in syntopic species of Phalloceros fishes (Poeciliidae)?

Abstract

The elucidation of mechanisms responsible for the reproductive isolation of species is a fundamental part of speciation research. South American poeciliid fishes in the genus Phalloceros represent a promising system in which to study the evolution of reproductive barriers between closely related species. Phalloceros are often found in sympatry with non-sister congeners, and most such species pairs have morphologically divergent female and male genitalia. In recent studies, it has been hypothesized that mismatched genitalia between co-occurring Phalloceros species might act as mechanical barriers to prevent hybridization and might help to explain the diversification of this group. However, this idea has not yet been evaluated empirically with genetic data. Here, we tested this hypothesis using morphological data in conjunction with mitochondrial and nuclear DNA sequence data. Our study focused on Phalloceros anisophallos and Phalloceros leptokeras, two non-sister species that have mismatched genitalia and that occur together in at least four rivers in south-eastern Brazil. Despite the prevalence of hybridization between closely related non-sister species of animals, especially fish, our phylogenetically-based results detected no evidence of mitonuclear discordance and (hence historical hybridization) between the two focal species. Our findings are therefore consistent with the hypothesis that mismatched genitalia prevent hybridization between sympatric species of Phalloceros fishes.