Do nitrogen- and sulphur-remobilization-related parameters measured at the onset of the reproductive stage provide early indicators to adjust N and S fertilization in oilseed rape (Brassica napus L.) grown under N- and/or S-limiting supplies?

Akmouche, Yacine; Chèneby, Jeanne; Lamboeuf, Mickael; Elie, Nicolas; Laperche, Anne; Bertheloot, Jessica; D'Hooghe, Philippe; Trouverie, Jacques; Avice, Jean‐Christophe; Étienne, Philippe; Brunel‐Muguet, Sophie

doi:10.1007/s00425-019-03284-2

Cited by 15 publications

(11 citation statements)

References 63 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Maintaining the yield and quality of harvested products to support the food needs of the human population and reducing the use of inputs while managing the influence of climate change on nutrient availability requires a better understanding of plant responses to nutritional deficiencies and earlier diagnosis of their occurrence. This is especially true for crop species such as Brassica napus , which are highly demanding of fertilizers [ 42 , 43 , 44 ]. In this context, the objective of this study was to provide early insights into the major molecular changes in Brassica napus subjected to six individual macronutrient (N, Mg, P, S, K, and Ca) deprivations, with the aim of assessing them before they initiate large macroscopic changes (growth and photosynthesis).…”

Section: Discussionmentioning

confidence: 99%

Comparative Omics Analysis of Brassica napus Roots Subjected to Six Individual Macronutrient Deprivations Reveals Deficiency-Specific Genes and Metabolomic Profiles

Courbet

D’Oria

Maillard³

et al. 2021

IJMS

Self Cite

View full text Add to dashboard Cite

The early and specific diagnosis of a macronutrient deficiency is challenging when seeking to better manage fertilizer inputs in the context of sustainable agriculture. Consequently, this study explored the potential for transcriptomic and metabolomic analysis of Brassica napus roots to characterize the effects of six individual macronutrient deprivations (N, Mg, P, S, K, and Ca). Our results showed that before any visual phenotypic response, all macronutrient deprivations led to a large modulation of the transcriptome and metabolome involved in various metabolic pathways, and some were common to all macronutrient deprivations. Significantly, comparative transcriptomic analysis allowed the definition of a subset of 3282, 2011, 6325, 1384, 439, and 5157 differentially expressed genes (DEGs) specific to N, Mg, P, S, K, and Ca deprivations, respectively. Surprisingly, gene ontology term enrichment analysis performed on this subset of specific DEGs highlighted biological processes that are common to a number of these macronutrient deprivations, illustrating the complexity of nutrient interactions. In addition, a set of 38 biochemical compounds that discriminated the macronutrient deprivations was identified using a metabolic approach. The opportunity to use these specific DEGs and/or biochemical compounds as potential molecular indicators to diagnose macronutrient deficiency is discussed.

show abstract

Section: Discussionmentioning

confidence: 99%

Comparative Omics Analysis of Brassica napus Roots Subjected to Six Individual Macronutrient Deprivations Reveals Deficiency-Specific Genes and Metabolomic Profiles

Courbet

D’Oria

Maillard³

et al. 2021

IJMS

Self Cite

View full text Add to dashboard Cite

show abstract

“…Sulphate was extracted from ca. 25 mg of freeze dried matter (DM), which was initially mixed up with 1 mL of 50% ethanol [18]. After incubation at 45 • C for 1 h, the extract was centrifuged at 10,000g for 10 min, and the supernatant was collected.…”

Section: Metabolite Profiling and Sulphate Measurementsmentioning

confidence: 99%

Autophagy Controls Sulphur Metabolism in the Rosette Leaves of Arabidopsis and Facilitates S Remobilization to the Seeds

Lornac

Havé

Chardon

et al. 2020

Cells

Self Cite

View full text Add to dashboard Cite

Sulphur deficiency in crops became an agricultural concern several decades ago, due to the decrease of S deposition and the atmospheric sulphur dioxide emissions released by industrial plants. Autophagy, which is a conserved mechanism for nutrient recycling in eukaryotes, is involved in nitrogen, iron, zinc and manganese remobilizations from the rosette to the seeds in Arabidopsis thaliana. Here, we have compared the role of autophagy in sulphur and nitrogen management at the whole plant level, performing concurrent labelling with 34S and 15N isotopes on atg5 mutants and control lines. We show that both 34S and 15N remobilizations from the rosette to the seeds are impaired in the atg5 mutants irrespective of salicylic acid accumulation and of sulphur nutrition. The comparison in each genotype of the partitions of 15N and 34S in the seeds (as % of the whole plant) indicates that the remobilization of 34S to the seeds was twice more efficient than that of 15N in both autophagy mutants and control lines under high S conditions, and also in control lines under low S conditions. This was different in the autophagy mutants grown under low S conditions. Under low S, the partition of 34S to their seeds was indeed not twice as high but similar to that of 15N. Such discrepancy shows that when sulphate availability is scarce, autophagy mutants display stronger defects for 34S remobilization relative to 15N remobilization than under high S conditions. It suggests, moreover, that autophagy mainly affects the transport of N-poor S-containing molecules and possibly sulphate.

show abstract

“…This opens new avenues for plant breeding via directional artificial selection of rhizosphere microbiota [1][2][3][4] . Still, while previous studies reported significant selection effects 6,7,11 , the selected property may not be perennial and lost during the process 6,9 . From a practical point of view, it is thus crucial to understand the causes behind this inconsistency.…”

Section: Main Textmentioning

confidence: 77%

“…We artificially selected rhizosphere microbiota according to their impact on a leaf greenness index, a remote proxy for plant nutritional and health status 9 (Fig. S1), using the grass species Brachypodium distachyon grown in microcosms in a climatic chamber (Fig.…”

Section: Main Textmentioning

confidence: 99%

Artificial selection of stable rhizosphere microbiota leads to heritable plant phenotype changes

Jacquiod

Spor

Shi

et al. 2021

Preprint

View full text Add to dashboard Cite

Research on artificial selection of microbial community has become popular due to perspectives in improving plant and animal health1-4. However, reported results still lack consistency5-8. We hypothesized that artificial selection may provide desired outcomes provided that microbial community structure has stabilized along the selection process. In a ten-generation artificial selection experiment involving 1,800 plants, we selected rhizosphere microbiota of Brachypodium distachyon that were associated with high or low levels of leaf greenness, a proxy for plant health9. Monitoring of the rhizosphere microbiota dynamics showed strong oscillations in community structure during an initial transitory phase of five generations, with no heritability in the selected property. In the last five generations, the structure of microbial communities displayed signs of stabilization, concomitantly to the appearance of heritability in leaf greenness. Selection pressure, initially ineffective, became successful in changing the greenness index in the intended direction, especially toward high greenness values. We showed a remarkable congruence between plant traits and selected microbial community structures, highlighting two phylogenetically distinct microbial sub-communities correlating with leaf greenness, whose abundance was significantly steered by directional artificial selection. Understanding microbial community structure stabilization can thus help improve the reliability of artificial microbiota selection.

show abstract

Do nitrogen- and sulphur-remobilization-related parameters measured at the onset of the reproductive stage provide early indicators to adjust N and S fertilization in oilseed rape (Brassica napus L.) grown under N- and/or S-limiting supplies?

Cited by 15 publications

References 63 publications

Comparative Omics Analysis of Brassica napus Roots Subjected to Six Individual Macronutrient Deprivations Reveals Deficiency-Specific Genes and Metabolomic Profiles

Comparative Omics Analysis of Brassica napus Roots Subjected to Six Individual Macronutrient Deprivations Reveals Deficiency-Specific Genes and Metabolomic Profiles

Autophagy Controls Sulphur Metabolism in the Rosette Leaves of Arabidopsis and Facilitates S Remobilization to the Seeds

Artificial selection of stable rhizosphere microbiota leads to heritable plant phenotype changes

Contact Info

Product

Resources

About