Do non-steroidal anti-inflammatory drugs affect the release of GnRH via neuronal markers?

Abstract

Purpose Some evidence that non-steroidal anti-inflammatory drugs have neuroprotective effects indicates their potential for use in a new field. However, its effects on hormone secretion have not been adequately discovered. Therefore, we aimed to evaluate the effects of dipyrone and indomethacin on neuronal markers as well as the GnRH expression in the GT1-7 cell line. Methods The effects of these drugs were evaluated by MTT analysis on proliferation. The effect of 10-50-250 µM concentrations of the drugs also on the expression of neuronal factors and markers, including NGF, nestin and βIII Tubulin, and additionally GnRH, was determined by the RT-qPCR method. Results NGF and nestin mRNA expressions were increased in all concentrations of both dipyrone and indomethacin. No changes were detected in βIII Tubulin. While dipyrone showed an increase in GnRH mRNA expression, there was no change at 10 and 50 µM concentrations of indomethacin, but a remarkable decrease was observed at 250 µM concentrations. Conclusions The results of our study showing an increase in the expression of neuronal factors reveal that dipyrone and indomethacin may have possible neuroprotective effects. Moreover, the effects on the GnRH expression appear to be different. Animal models are required to confirm these effects of NSAIDs on neurons.