Do penile haemodynamics change in the presence of hydrogen sulphide (H₂S) donor in metabolic syndrome-induced erectile dysfunction?

Abstract

Erectile dysfunction (ED) is defined in relation to the metabolic syndrome (metS). Hydrogen sulphide (H S), a gasotransmitter, has been revealed to get involved in hypertension, insulin secretion and regulation of vascular tone especially in erectile physiology. This study aimed to investigate the effect of H S on metS-induced ED. Animals were divided into two groups as control and metS, which were fed with standard diet or 60% high-fructose diet for 10 weeks respectively. The metS model was evaluated with biochemical analyses, waist circumference/tibia length ratio and HOMA index. Penile hemodynamic parameters were evaluated by the measurement of intracavernous pressure/mean arterial pressure ratio during cavernous nerve stimulation in the presence and absence of intracavernous injection of NaHS (100 μg/50 μl) and its control 0.9%NaCl (50 μl) in both groups. H S levels were measured in penile tissues by methylene blue assay. H S levels were significantly decreased in the penile tissues of the metS group. Decreased intracavernous pressure/mean arterial pressure ratio improved after intracavernous administration of NaHS in the metS group. These results suggest the significant role of H S in the metS-induced erectile dysfunction that could be a new therapeutic target.

Sodium Tanshinone IIA Sulfonate Attenuates Erectile Dysfunction in Rats with Hyperlipidemia

“…We demonstrated that H 2 S production in penile tissues of rats with hyperlipidemia-associated ED was decreased, and STS treatment could alleviate the change. Similar to our findings, decreased H 2 S generation in penile tissues was accompanied by impaired erectile function, indicating the importance of H 2 S in maintaining sexual function [24,25]. Our results also indicated that the expression of CSE and CBS was decreased in the HFD group, and STS treatment increased expression of these two H 2 S-generating enzymes, which can account for the H 2 S production changes.…”

Vascular Effects of Low-Dose ACE2 Inhibitor MLN-4760—Benefit or Detriment in Essential Hypertension?

“…H 2 S concentration was measured in plasma and heart tissue via methylene blue as-say as described previously [ 28 , 29 ]. To assess H 2 S production, 75μL of plasma combined with 500 μL of reaction mixture containing 0.1 mol/L potassium phosphate buffer (325 μL), substrate, and cofactor of H 2 S that are l -cysteine and pyridoxal-5-phosphate, respectively.…”

Hydrogen Sulfide in Pharmacotherapy, Beyond the Hydrogen Sulfide-Donors

“…We have recently shown that, besides tissue enzymes, the gut microbiota-derived H 2 S may be involved in the development of hypertension [83,89,96]. In addition, H 2 S donors were found to relax corpus cavernosum and were tested for the treatment of erectile dysfunction [97][98][99][100].…”