The present study was conducted to determine the proximate composition, glycemic indices (GIs), and some physicochemical properties of starch of boiled tubers of Dioscorea alata (violet and white), Maranta arundinacea, and Canna indica with the intention of recommending these for consumption or utilization as an ingredient in food preparation. Proximate compositions of cooked tubers were determined according to standard methods. GIs were determined according to FAO/WHO guidelines with bread as the standard. The size and shape of the starch granules and the carbohydrate molecular size distribution were studied to correlate their effects to individual GIs. Digestible carbohydrate ranged from 59.0 to 69.1 g/100 g dry weight (DW) and was not significantly (p > 0.05) different among the tubers. Crude protein content ranged from 4.5 to 9.2 g/100 g DW with Dioscorea (violet) and Maranta having the highest amounts, 9.2 and 8.6%, respectively. Fat contents were low (1.2–2.3 g/100 g DW). The contribution by insoluble and soluble fiber ranged from 7.0 to 11.0 and 4.0 to 6.0 g/100 g DW, respectively. Dioscorea tubers (violet and white) were categorized as low GI, with corresponding GI's of (±SEM) 64 ± 9 and 69 ± 4, respectively. Maranta was categorized as a medium GI (82 ± 8) and Canna as a high GI (110 ± 8) food. Microscopic studies and carbohydrate distribution patterns indicated cell enclosed starch granules in the two Dioscorea varieties, which have not been reported earlier. It is concluded that, low GI yams (Dioscorea white and violet) have a high potential to be utilized as a functional ingredient in food formulations targeting individuals who require glycemic control.