Docosahexaenoic acid (DHA), an essential fatty acid for the proper functioning of neuronal cells: their role in mood disorders

Valenzuela, Beatriz

doi:10.3989/gya.085208

Cited by 38 publications

(35 citation statements)

References 84 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Polyunsaturated fatty acids (PUFAs) such as docosahexanoic acid (DHA,) and eicosapentaenoic acid (EPA,), have beneficial effects on human health through their effect on membrane fluidity and contribute to many aspects of health, including but not limited to, fetal development and prevention of cancer and obesity (Chapkin et al 2008;Connor et al 2000;Lagarde 2008;Li et al 2008;Siddiqui et al 2008;Valenzuela 2009). It is also established that these compounds downregulate inflammatory pathways that are known to be activated in chronic diseases such as rheumatoid arthritis, obesity and type 2 diabetes, among others (Wall et al 2010).…”

Section: Introductionmentioning

confidence: 99%

Growth condition optimization for docosahexaenoic acid (DHA) production by Moritella marina MP-1

Kautharapu

Rathmacher

Jarboe

2012

Appl Microbiol Biotechnol

View full text Add to dashboard Cite

The marine organism Moritella marina MP-1 produces the polyunsaturated fatty acid docosahexaenoic acid (DHA). While the basic metabolic pathway for DHA production in this organism has been identified, the impact of growth conditions on DHA production is largely unknown. This study examines the effect of supplemental carbon, nitrogen and salts, growth temperature and media composition and pH on DHA and biomass production and the fatty acid profile. The addition of supplemental nitrogen significantly increased the overall DHA titer via an increase in biomass production. Supplemental glucose or glycerol increased biomass production, but decreased the amount of DHA per biomass, resulting in no net change in the DHA titer. Acidification of the baseline media pH to 6.0 increased DHA per biomass. Changes in growth temperature or provision of supplemental sodium or magnesium chloride did not increase DHA titer. This organism was also shown to grow on defined minimal media. For both media types, glycerol enabled more DHA production per biomass than glucose. Combination of these growth findings into marine broth supplemented with glycerol, yeast extract, and tryptone at pH 6.0 resulted in a final titer of 82 Â± 5 mg/L, a nearly eightfold increase relative to the titer of 11 Â± 1 mg/L seen in the unsupplemented marine broth. The relative distribution of other fatty acids was relatively robust to growth condition, but the presence of glycerol resulted in a significant increase in myristic acid (C14:0) and decrease in palmitic acid (C16:0). In summary, DHA production by M. marina MP-1 can be increased more than fivefold by changing the growth media. Metabolic engineering of this organism to increase the amount of DHA produced per biomass could result in additional increases in titer. ABSTRACTThe marine organism Moritella marina MP-1 produces the polyunsaturated fatty acid docosahexaenoic acid (DHA). While the basic metabolic pathway for DHA production in this organism has been identified, the impact of growth conditions on DHA production is largely unknown. This study examines the effect of supplemental carbon, nitrogen and salts, growth temperature and media composition and pH on DHA and biomass production and the fatty acid profile. The addition of supplemental nitrogen significantly increased the overall DHA titer via an increase in biomass production. Supplemental glucose or glycerol increased biomass production, but decreased the amount of DHA per biomass, resulting in no net change in the DHA titer.Acidification of the baseline media pH to 6.0 increased DHA per biomass. Changes in growth temperature or provision of supplemental sodium or magnesium chloride did not increase DHA titer. This organism was also shown to grow on defined minimal media. For both media types, glycerol enabled more DHA production per biomass than glucose. Combination of these growth findings into marine broth supplemented with glycerol, yeast extract and tryptone at pH 6.0 resulted in a final titer of 82+5 mg/L, a nearly 8-fold increase...

show abstract

Section: Introductionmentioning

confidence: 99%

Growth condition optimization for docosahexaenoic acid (DHA) production by Moritella marina MP-1

Kautharapu

Rathmacher

Jarboe

2012

Appl Microbiol Biotechnol

View full text Add to dashboard Cite

show abstract

“…Future clinical research and nutritional interventions should be planned directly to establish the necessary doses of DHA needed to achieve significant beneficial effects, as well as to encourage the development and consumption of foods and/or supplements rich in this fatty acid. In this regard, the development of functional foods and/or nutraceuticals containing DHA at different concentrations is an alternative that the pharmaceutical and the food industries should consider very seriously (Valenzuela et al, 2009). To day the increase of the consumption of fish or seafood appear as not entirely feasible, due to the massive depredation of the resource, which has decreased its availability and consequently has increased the price of the products from the sea.…”

Section: Discussionmentioning

confidence: 99%

Docosahexaenoic Acid (DHA), in the Prevention and Treatment of Neurodegenerative Diseases

Valenzuela¹,

Valenzuela²

2011

Neurodegenerative Diseases - Processes, Prevention, Protection and Monitoring

View full text Add to dashboard Cite

“…DHA is present in large proportion in the grey matter of the brain's cerebral cortex, which constitutes 15-20% of the total fatty acids in the brain. It is believed that DHA plays an important role in regulating the fluidity of neuronal membranes, and thus the efficiency of neuroelectrical signal transmissions (Marszalek & Lodish, 2005;Valenzuela, 2009). In humans, the highest amount of DHA is produced during the last trimester of pregnancy and also during the first two years of life (Clandinin, 1999;Wainwright, 2002).…”

Section: Animals Diet and Supplementing Protocolsmentioning

confidence: 99%

Supplementing female rats with DHA-lysophosphatidylcholine increases docosahexaenoic acid and acetylcholine contents in the brain and improves the memory and learning capabilities of the pups.

Valenzuela¹,

Nieto²,

Sanhueza³

et al. 2009

Grasas y Aceites

View full text Add to dashboard Cite

DHA has been established. We propose that DHAcontaining lysophosphatidylcholine (DHA-LPC), obtained from DHA-rich eggs may be a suitable form of DHA and choline (the precursor of acetylcholine) supplementation. We evaluated the effectiveness of DHA-LPC to increase DHA and acetylcholine concentration in the brain of pups born from female rats supplemented with DHA-LPC before and during pregnancy. We also evaluated the effect of DHA supplementation on learning and memory capabilities of pups through the Skinner test for operant conditioning. Female Wistar rats received 40-day supplementation of DHA-LPC (8 mg DHA/kg b.w/daily.), before and during pregnancy. After delivery, plasma, erythrocyte, liver, and adipose tissue DHA and plasma choline were analyzed. Brains from 60 day-old pups separated into frontal cortex, cerebellum, striatum, hippocampus, and occipital cortex, were assessed for DHA, acetylcholine, and acetylcholine transferase (CAT) activity. Pups were subjected to the Skinner box test. DHA-LPC supplementation produces higher choline and liver DHA contents in the mother's plasma and increases the pups' DHA and acetylcholine in the cerebellum and hippocampus. CAT was not modified by supplementation. The Skinner test shows that pups born from DHA-LPC supplemented mothers exhibit better scores of learning and memory than the controls. Conclusion: DHA-LPC may be an adequate form for DHA supplementation during the perinatal period. KEY-WORDS: Brain acetylcholine -Choline -DHA supplementation -Lysophospholipids -Skinner box test.

show abstract

Docosahexaenoic acid (DHA), an essential fatty acid for the proper functioning of neuronal cells: their role in mood disorders

Cited by 38 publications

References 84 publications

Growth condition optimization for docosahexaenoic acid (DHA) production by Moritella marina MP-1

Growth condition optimization for docosahexaenoic acid (DHA) production by Moritella marina MP-1

Docosahexaenoic Acid (DHA), in the Prevention and Treatment of Neurodegenerative Diseases

Supplementing female rats with DHA-lysophosphatidylcholine increases docosahexaenoic acid and acetylcholine contents in the brain and improves the memory and learning capabilities of the pups.

Contact Info

Product

Resources

About