Docosahexaenoic Acid (DHA, C22:6, ω-3) Composition of Milk and Mammary Gland Tissues of Lactating Mother Rats Is Severely Affected by Lead (Pb) Exposure

Hossain, Shahdat; Hussain, Jakir; Bhowmick, Sujan; Sarkar, Marzan; Basunia, Mafroz Ahmed; Mamun, Abdullah Al; Tanabe, Yoko; Matsuzaki, Kei; Hashimoto, Michio; Shido, Osamu

doi:10.1007/s12011-019-01878-1

Cited by 7 publications

(7 citation statements)

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“…Polyunsaturated fatty acids are susceptible to degradation by oxygen, heat, and light, and their presence increases the potential for lipid peroxidation [ 51 ]. The decrease in the C18:2n6c content in polluted areas may be secondary to the impact of lipid peroxidation caused by heavy metals [ 23 , 52 ].Heavy metals alter fatty acid synthesis through oxidative stress [ 23 ], which may be the process by which heavy metals affect fatty acid synthesis in milk [ 27 ].…”

Section: Resultsmentioning

confidence: 99%

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Effects of Heavy Metal Exposure from Leather Processing Plants on Serum Oxidative Stress and the Milk Fatty Acid Composition of Dairy Cows: A Preliminary Study

Gao

et al. 2022

Animals

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This study investigated whether unsaturated fatty acids in milk and the oxidative status of cows are affected by heavy metal exposure due to leather processing. The blood lead (Pb) concentrations in cows from two farms in the polluted area were 16.27 ± 8.63 μg/L, respectively, which were significantly (p < 0.05) higher than the blood Pb concentrations in cows from an unpolluted farm (6.25 ± 3.04 μg/L). There were significantly (p < 0.05) lower levels of glutathione S-transferase (GST), glutathione peroxidase (GPX), and glutathione (GSH) in the serum of cows from the polluted area compared to the levels in cows from an unpolluted area. The linoleic acid (C18:2n6c) content in milk from the polluted area was 15% lower than in the control area. There was a significant correlation between linoleic acid in milk with the blood Pb and serum GSH levels. Heavy metals can alter fatty acid synthesis through oxidative stress, which may be the mechanism by which heavy metals affect fatty acid synthesis in milk.

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Section: Resultsmentioning

confidence: 99%

“…Several studies have shown a relationship between elevated tissue Pb and both oxidative stress biomarkers and fatty acid composition [ 23 , 24 , 25 , 26 ]. Lead exposure has been shown to alter the ω-3 polyunsaturated fatty acids (PUFAs) and ω-6 PUFAs composition of milk in rats [ 27 ].…”

Section: Introductionmentioning

confidence: 99%

Effects of Heavy Metal Exposure from Leather Processing Plants on Serum Oxidative Stress and the Milk Fatty Acid Composition of Dairy Cows: A Preliminary Study

Gao

et al. 2022

Animals

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“…Pb exposure in lactating dams resulted in significantly decreased total monounsaturated fatty acids and total fatty acid concentrations; in particular, reduced levels of dihomo-gamma-linolenic acid (DGLA), linoleic acid (LA), and eicosapentaenoic acid (EPA) were observed in plasma (41). Further, rats exposed to Pb during pregnancy and lactation had reduced DHA in milk and mammary gland tissues (42), and in pups, Pb exposure in dam’s milk, in combination with an n -3 insufficient diet, resulted in a reduction in total saturated fatty acids and total n -3 and total n -6 fatty acids in plasma i (43). Interestingly, the relationship may be bidirectional: a lower blood Pb concentration was observed in rats supplemented with a diet rich in n -3 and n -6 polyunsaturated fatty acids (44).…”

Section: Discussionmentioning

confidence: 99%

Lead exposure and serum metabolite profiles in pregnant women in Mexico City

Niedzwiecki

Eggers

Joshi

et al. 2021

Preprint

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Background: Lead (Pb) exposure is a global health hazard causing a wide range of adverse health outcomes. Yet, the mechanisms of Pb toxicology remain incompletely understood, especially during pregnancy. To uncover biological pathways impacted by Pb exposure, this study investigated serum metabolomic profiles during the third trimester of pregnancy that are associated with blood Pb and bone Pb. Methods: We used data and specimens collected from 99 women enrolled in the Programming Research in Obesity, Growth, Environment, and Social Stressors birth cohort based in Mexico City. Maternal Pb exposure was measured in whole blood samples from the third trimester of pregnancy and in the tibia and patella bones at 1 month postpartum. Third-trimester serum samples underwent metabolomic analysis; metabolites were identified based on matching to an in-house analytical standard library. A metabolome-wide association study was performed with all three Pb measurements using multiple linear regression models, adjusted for confounders and batch effects. Class enrichment analyses were also conducted. Results: The median (interquartile range) blood Pb concentration was 2.9 (2.6) μg/dL. Median bone Pb, measured in the patella and tibia, were 2.5 (7.3) μg/g and 3.6 (9.5) μg/g, respectively. Of 248 total metabolites identified in serum, 31 were associated with blood Pb (p<0.05). Class enrichment analysis identified significant overrepresentation of metabolites classified as fatty acids and conjugates, amino acids and peptides, and purines. Tibia and patella Pb were associated with 14 and 11 metabolites, respectively (p<0.05). Comparing results from bone and blood Pb, glycochenodeoxycholic acid and glycocholic acid were negatively associated with blood Pb and tibia Pb, while 5-aminopentanoic acid and 7-methylguanine were negatively associated with blood Pb and patella Pb. One metabolite, 5-aminopentanoic acid, was associated with all three Pb measures. Conclusion: This study identified serum metabolites in pregnant women associated with Pb measured in blood (31 metabolites) and bone (tibia: 14 metabolites, patella: 11 metabolites). These findings provide insights on the metabolic profile around Pb exposure in pregnancy and may provide important links to guide detailed studies of toxicological effects for both mothers and children.

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“…LPO levels, as indicator of OS, were assessed as previously described by Hossain et al, (). Briefly, 200 μl of 8.1% sodium dodecyl sulfate, 3.0 ml of 0.4% thiobarbituric acid in 20% acetic acid (pH 3.5), and 700 μl distilled water were added to 100 μl of homogenate; the mixture was then incubated at 95 °C for 1 hr.…”

Section: Methodsmentioning

confidence: 99%

“…TNFα levels were measured as described previously (Hossain, Bhowmick, Sarkar, Basunia, & Mamun, ). Briefly, the multi‐well plate coated with 20 μl of cytosolic fraction in 180 μl of 100 mM sodium bicarbonate buffer (pH 9.6) was incubated at 4 °C for 12 hr.…”

Section: Methodsmentioning

confidence: 99%

Lablab purpureus (L) bean flour ameliorates plasma proteins and accretion of docosahexaenoic acid (DHA, 22:6, ω‐3) in the plasma, liver, and brain of malnourished rats

et al. 2020

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Lablab purpureus (L) bean is an undervalued or underutilized orphan crop in many tropical countries, where different forms of malnutrition are associated with stunting growth and cognitive deficiencies. We previously reported that L. purpureus contains ω-3 α-linolenic acid (ALA, C18:3, ω-3), which can act as the precursor of ω-3 docosahexaenoic acid (DHA, C22:6, ω-3). Inadequate level of DHA impairs growth, development, and cognitive performance. Therefore, we evaluated if supplementation of L. purpureus seed flour (LPS) affects the nutritional status, in terms of body weight gain, plasma proteins, and DHA levels of malnourished model rats. Three groups of rats, namely, controls, malnourished alone (MN), and 15% LPS-supplemented malnourished (MN + LPS) rats were fed with LPS for 12 weeks. Afterward, body weight, liver weight, brain weight, plasma proteins, micronutrients, lipid profile, and fatty acid profile of plasma, liver, and brains were determined by standard methods. The levels of liver lipid peroxide (LPO) and proinflammatory TNFα were also measured. The body weight, liver weight, serum total proteins and micronutrients (iron/potassium), and the levels of docosahexaenoic acid significantly increased in the plasma, liver, and brain of MN + LPS rats. Moreover, LPO and TNFα levels reduced significantly in MN + LPS rats. in vitro analysis revealed a significant free radical scavenging and antioxidative potential of L. purpureus seed extract. Thus, L. purpureus not only replenishes protein-energy malnutrition, but also increases the levels of DHA, an indispensable polyunsaturated fatty acid for brain cognition. Finally, our results suggest that L. purpureus might benefit human malnutrition and related cognitive deficits. K E Y W O R D Sbrain, docosahexaenoic acid (DHA), Lablab purpureus, malnutrition

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Docosahexaenoic Acid (DHA, C22:6, ω-3) Composition of Milk and Mammary Gland Tissues of Lactating Mother Rats Is Severely Affected by Lead (Pb) Exposure

Cited by 7 publications

References 66 publications

Effects of Heavy Metal Exposure from Leather Processing Plants on Serum Oxidative Stress and the Milk Fatty Acid Composition of Dairy Cows: A Preliminary Study

Effects of Heavy Metal Exposure from Leather Processing Plants on Serum Oxidative Stress and the Milk Fatty Acid Composition of Dairy Cows: A Preliminary Study

Lead exposure and serum metabolite profiles in pregnant women in Mexico City

Lablab purpureus (L) bean flour ameliorates plasma proteins and accretion of docosahexaenoic acid (DHA, 22:6, ω‐3) in the plasma, liver, and brain of malnourished rats

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