Docosahexaenoic acid inhibition of inflammation is partially via cross-talk between Nrf2/heme oxygenase 1 and IKK/NF-κB pathways

N-Propargyl Caffeamide Skews Macrophages Towards a Resolving M2-Like Phenotype Against Myocardial Ischemic Injury via Activating Nrf2/HO-1 Pathway and Inhibiting NF-ĸB Pathway

“…In the present study, therefore, we hypothesized that PACA might regulate iNOS induction and macrophage M2 polarization via activating Nrf2/HO-1 pathway. HO-1 is well-known to orchestrate the cross-talks between Nrf2 and NF-kB pathways [14,44,45]. Thus, we further examined whether HO-1 inhibitor SnPP could affect the activity of PACA.…”

Shikonin inhibits oxidized LDL-induced monocyte adhesion by suppressing NFκB activation via up-regulation of PI3K/Akt/Nrf2-dependent antioxidation in EA.hy926 endothelial cells

“…The subsequent transfection experiment was performed with Nanofectin reagent (PAA, Pasching, Austria) with some modifications as described previously [30]. Briefly, cells were transiently transfected with 0.1 mg of pGL3-2Â ARE/Luc plasmid and 0.1 mg of pCMV-b-galactosidase plasmid by using 0.4 ml of Nanofectin in OPTI-MEM medium for 16 h. After transfection, cells were changed to DMEM medium and treated with shikonin for an additional 16 h. The luciferase activity was measured by using a luciferase substrate kit (Promega, Madison, WI) in a microplate luminometer (Tropix TR-717, Applied Biosystems).…”

Induction of heme oxygenase-1 and inhibition of TPA-induced matrix metalloproteinase-9 expression by andrographolide in MCF-7 human breast cancer cells

“…RNA isolation was performed according to our previous study (29). MMP-9, HO-1 and glyceraldehyde-3-phosphate dehydrogenase messenger RNA (mRNA) expression were determined by use of the MiniOpticon Real-Time PCR System (Bio-Rad Laboratories, Hercules, CA).…”

“…Nuclear protein preparation was performed as described previously (31), and western blotting analysis as well as electrophoretic mobility shift assay were performed according to our previous study (29). Synthetic biotin-labeled doublestranded AP-1 consensus oligonucleotides (forward: 5ʹ-GCCTCAGCTGGT AAATGGATAA-3ʹ; reverse: 5ʹ-AAAGGCCCCAGAGCCAGCC-3ʹ) were used to measure AP-1 nuclear protein DNA binding activity and synthetic biotinlabeled double-stranded NF-κB consensus oligonucleotides (forward: 5ʹ-AGT TGAGGGGACTTTCCCAGGC-3ʹ; reverse: 5ʹ-GCCTGGGAAAGTCCC CTCAACT-3ʹ) were used to measure NF-κB nuclear protein DNA binding activity.…”