Documentation of fungal endophytes of black pepper (Piper nigrum L.) and their seed transmission studies

Sreeja, K; Anandaraj, M.; Bhai, R. Suseela

doi:10.25081/josac.2019.v28.i2.6074

Cited by 7 publications

(9 citation statements)

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“…Similarly, Mandlaa et al (2019) et al, 2000). However, Sreeja et al (2019) reported that the endocarp of black pepper seed is free from fungal endophytes.…”

Section: Discussionmentioning

confidence: 99%

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In Vitro and In Silico Antioxidant Efficiency of Bio-Potent Secondary Metabolites From Different Taxa of Black Seed-Producing Plants and Their Derived Mycoendophytes

Hassane

Hussien

Abouelela

et al. 2022

Front. Bioeng. Biotechnol.

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Oxidative stress is involved in the pathophysiology of multiple health complications, and it has become a major focus in targeted research fields. As known, black seeds are rich sources of bio-active compounds and widely used to promote human health due to their excellent medicinal and pharmaceutical properties. The present study investigated the antioxidant potency of various black seeds from plants and their derived mycoendophytes, and determined the total phenolic and flavonoid contents in different extracts, followed by characterization of major constituents by HPLC analysis. Finally, in silico docking determined their binding affinities to target myeloperoxidase enzymes. Ten dominant mycoendophytes were isolated from different black seed plants. Three isolates were then selected based on high antiradical potency and further identified by ITS ribosomal gene sequencing. Those isolated were Aspergillus niger TU 62, Chaetomium madrasense AUMC14830, and Rhizopus oryzae AUMC14823. Nigella sativa seeds and their corresponding endophyte A. niger had the highest content of phenolics in their n-butanol extracts (28.50 and 24.43 mg/g), flavonoids (15.02 and 11.45 mg/g), and antioxidant activities (90.48 and 81.48%), respectively, followed by Dodonaea viscosa and Portulaca oleracea along with their mycoendophytic R. oryzae and C. madrasense. Significant positive correlations were found between total phenolics, flavonoids, and the antioxidant activities of different tested extracts. The n-butanol extracts of both black seeds and their derived mycoendophytes showed reasonable IC50 values (0.81–1.44 mg/ml) compared to the control with significant correlations among their phytochemical contents. Overall, seventeen standard phenolics and flavonoids were used, and the compounds were detected in different degrees of existence and concentration in the examined extracts through HPLC analysis. Moreover, the investigation of the molecular simulation results of detected compounds against the myeloperoxidase enzyme revealed that, as a targeted antioxidant, rutin possessed a high affinity (−15.3184 kcal/mol) as an inhibitor. Taken together, the black seeds and their derived mycoendophytes are promising bio-prospects for the broad industrial sector of antioxidants with several valuable potential pharmaceutical and nutritional applications.

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“…Similarly, Mandlaa et al (2019) et al, 2000). However, Sreeja et al (2019) reported that the endocarp of black pepper seed is free from fungal endophytes.…”

Section: Discussionmentioning

confidence: 99%

“…was recovered from Amaranthus hybridus seeds ( Blodjett et al, 2000 ). However, Sreeja et al (2019) reported that the endocarp of black pepper seed is free from fungal endophytes.…”

Section: Discussionmentioning

confidence: 99%

In Vitro and In Silico Antioxidant Efficiency of Bio-Potent Secondary Metabolites From Different Taxa of Black Seed-Producing Plants and Their Derived Mycoendophytes

Hassane

Hussien

Abouelela

et al. 2022

Front. Bioeng. Biotechnol.

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“…Competition, antibiosis, and mycoparasitism may be some of the disease suppressing features of antagonists. [16][17][18][19] *Mean (± SD) of four replications (n=60). Values in a column followed by the same alphabet do not differ significantly (p≤0.05).…”

Section: Discussionmentioning

confidence: 99%

Bacterization with Endospore-forming Bacillus spp. Promotes Plant Growth and Suppresses Foot Rot Disease in Black Pepper (Piper nigrum L.) in the Nursery

Anju¹,

Natarajan²,

Preetha³

et al. 2023

J. Pure Appl. Microbiol.

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Foot rot disease (Phytophthora capsici) causes severe economic losses to cultivators of black pepper. Fungicides used for managing the disease adversely affect the export potential of the product due to their residual toxicity. Endospore-forming bacterial strains, Bacillus amyloliquefaciens VLY24, Bacillus pumilus VLY17, and Bacillus velezensis PCSE10 were evaluated for growth promotion and disease suppression in black pepper nursery at two locations in Kerala, India. All the isolates could suppress Phytophthora capsici under in vitro conditions. Spraying detached leaves with a cell suspension of the bacterial isolates could delay the development and progression of lesion. In the in vivo trials, bacterized cuttings had a better establishment in the nursery when compared to uninoculated control. Plant growth parameters like the number of leaves, roots, and shoots were more in bacterized cuttings. Cuttings treated with Bacillus pumilus VLY17 when challenge inoculated with the pathogen, showed 84.74 percent reduction in the size of lesions on the leaves, and showed the least disease index (0.27) compared to pathogen inoculated control. It is proposed that endospore forming Bacillus spp. with anti-oomycetic activity could be potential biocontrol agents against foot rot disease of black pepper.

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“…suggested that the different pharmacological results of plants grown aseptically and those grown naturally are possibly due to the effect of the presence of endophytic fungi, in which they affected the production of bioactive metabolites [10] . The leaves, fruits, particularly roots of P. nigrum are likely to be sources of fungal genera such as Fusarium and Penicillium [11,12] . Endophytic fungi normally produce a rich variety of bioactive substances with identical or similar pharmacological activities to those determined from plants [13] .…”

Section: Introductionmentioning

confidence: 99%

“…[10] The leaves, fruits, particularly roots of P. nigrum are likely to be sources of fungal genera such as Fusarium and Penicillium. [11,12] Endophytic fungi normally produce a rich variety of bioactive substances with identical or similar pharmacological activities to those determined from plants. [13] Therefore, it is believed that the fungal endophytes of P. nigrum are likely to produce multiple pharmaceutical properties as similar to the black pepper, especially antimicrobial, antioxidant, anti-inflammatory, antitumor, and antidiabetic.…”

Section: Introductionmentioning

confidence: 99%

Chemical Profile and Biological Activities of Fungal Strains Isolated fromPiper nigrumRoots: Experimental and Computational Approaches

Luyen

Hương

Hà

et al. 2023

Chemistry & Biodiversity

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The current report describes the chemical investigation and biological activity of extracts produced by three fungal strains Fusarium oxysporum, Penicillium simplicissimum, and Fusarium proliferatum isolated from the roots of Piper nigrum L. growing in Vietnam. These fungi were namely determined by morphological and DNA analyses. GC/MS identification revealed that the EtOAc extracts of these fungi were associated with the presence of saturated and unsaturated fatty acids. These EtOAc extracts showed cytotoxicity towards cancer cell lines HepG2, inhibited various microbacterial organisms, especially fungus Aspergillus niger and yeast Candida albicans (the MIC values of 50 -100 μg/mL). In α-glucosidase inhibitory assay, they induced the IC 50 values of 1.00-2.53 μg/mL were better than positive control acarbose (169.80 μg/mL). The EtOAc extract of F. oxysporum also showed strong anti-inflammatory activity against NO production and PGE-2 level. Four major compounds linoleic acid (37.346 %), oleic acid (27.520 %), palmitic acid (25.547 %), and stearic acid (7.030 %) from the EtOAc extract of F. oxysporum were selective in molecular docking study, by which linoleic and oleic acids showed higher binding affinity towards α-glucosidase than palmitic and stearic acids. In subsequent docking assay with inducible nitric oxide synthase (iNOS), palmitic acid, oleic acid and linoleic acid could be moderate inhibitors.

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Documentation of fungal endophytes of black pepper (Piper nigrum L.) and their seed transmission studies

Cited by 7 publications

References 0 publications

In Vitro and In Silico Antioxidant Efficiency of Bio-Potent Secondary Metabolites From Different Taxa of Black Seed-Producing Plants and Their Derived Mycoendophytes

In Vitro and In Silico Antioxidant Efficiency of Bio-Potent Secondary Metabolites From Different Taxa of Black Seed-Producing Plants and Their Derived Mycoendophytes

Bacterization with Endospore-forming Bacillus spp. Promotes Plant Growth and Suppresses Foot Rot Disease in Black Pepper (Piper nigrum L.) in the Nursery

Chemical Profile and Biological Activities of Fungal Strains Isolated fromPiper nigrumRoots: Experimental and Computational Approaches

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