Does a relative perfusion measure predict cerebral infarct size?

Engelhorn, Tobias; Doerfler, Arnd; Heusch, Gerd; Schulz, Richard; Forsting, Michael

doi:10.1055/s-2004-833229

Cited by 6 publications

(10 citation statements)

References 17 publications

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“…Indeed, stereotaxic ICV invasion and further infusion has been widely used and attempted under several different ischaemic conditions but rarely at 2-24 h post-occlusion. It has been performed either prior to MCAO where oedema is not a problem, 9 -12 early (15 -30 min up to 2 h) post-occlusion where oedema starts accumulating 12,30,31 or several days later (3 days up to 2 -3 weeks), where most of the oedema has been resolved. 8,13 However, to our knowledge, there have been only four attempts to stereotaxically invade the lateral ventricles 20 When interpreting the results in the present study, most of the neurological scores (mNSS, mBS and GWT) well correlated with the ventricular dislocations at 6 and 18 h post-occlusion (Table 1); however, only the mNSS score was finally selected for mathematical calculations, as it is more widely accepted in the literature.…”

Section: Discussionmentioning

confidence: 99%

Predictable ventricular shift after focal cerebral ischaemia in rats: practical considerations for intraventricular therapeutic interventions

et al. 2010

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Intracerebroventricular (ICV) route of administration is a useful experimental method to study the effects of chemicals or cellular grafts in the ventricular compartment of the brain after focal ischaemia. However, the induced oedema may cause structural dislocating phenomena and render a stereotaxic ICV invasion difficult and practically unavailable especially during the acute post-ischaemia phase. The aim of this study was to measure these structural ventricular dislocations and set new stereotaxic coordinates for successful and cost-effective ICV invasion 6-18 h after focal cerebral ischaemia. Wistar rats were subjected to 2 h middle cerebral artery occlussion (t-MCAO), were neurologically evaluated (modified Neurological Stroke Scale [mNSS], modified Bederson's Scale [mBS] and grid-walking test [GWT]) and brain slides were studied at 6 and 18 h post-occlusion for infarction volume, hemispheric oedema, middle line dislocation and stereotaxia of the lateral ventricles. Our data indicated that stereotaxic coordinates of the lateral ventricles in the infarcted and contralateral hemispheres significantly (P < 0.05) changed at both time-points and were linearly correlated with the mNSS, mBS and some GWT scores (P < 0.001). This correlation allowed for the calculation of simple (linear) mathematical equations (stereotaxic coordinate = b0 + b1*mNSS, where 'b0' and 'b1' are fixed number and factor, respectively, calculated by regression analysis) that determined individually new coordinates for each animal. Verification experiments revealed that the new coordinates render ICV invasion feasible in up to 80% of infarcted rats (number needed to treat 1.65), compared with only 19.4% using the classical coordinates for normal rats. Therefore, we propose a new, time- and cost-effective methodology for practically feasible ICV invasion in rats 6-18 h after t-MCAO.

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Section: Discussionmentioning

confidence: 99%

Predictable ventricular shift after focal cerebral ischaemia in rats: practical considerations for intraventricular therapeutic interventions

et al. 2010

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“…Transient focal cerebral ischemia was induced by intraluminal occlusion of the right middle cerebral artery (nϭ5 9 ). Infarct volume was measured according to Engelhorn et al 10 …”

Section: Transient Focal Cerebral Ischemiamentioning

confidence: 99%

MicroRNA Expression in the Blood and Brain of Rats Subjected to Transient Focal Ischemia by Middle Cerebral Artery Occlusion

Jeyaseelan

Lim

Armugam

2008

Stroke

532

436

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Background and Purpose-Several hundred small RNAs called microRNAs (miRNAs) have been identified and characterized from various organisms, including humans. In humans, some of these miRNAs have been found to regulate (patho)physiologic conditions such as tumor progression/regression, cholesterol and glucose homeostasis, etc.In this report, we present data on the miRNAs expressed under ischemic conditions in both the brain and blood of rats subjected to middle cerebral artery occlusion (MCAo). Methods-Sprague-Dawley rats subjected to MCAo were reperfused for either 24 or 48 hours, and both blood and brain samples were harvested. miRNA expression profiling and oligonucleotide microarray were carried out, and the data were validated by quantitative real-time polymerase chain reaction and correlated with published data on protein and gene expression in MCAo rats. Results-We report here for the first time the involvement of miRNA regulation in brain pathogenesis associated with MCAo. Comparison with the corresponding DNA microarray data revealed that the target mRNA expression is correlated with the regulation of miRNA. We have also provided evidence that some of the miRNAs that are highly expressed in the ischemic brain can be detected in blood samples. Conclusions-Further

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“…The mean value of CBF in the ROI Hypoxia (31.0 AE 1.4 ml/ 100 g/min) was no longer compatible with cell survival. 29 StO 2 fell to 22.0 AE 0.6%; in the ROI LesionD0 it was 47.0 AE 1.4%. BVf was also reduced (from 2.2 AE 0.7 to 1.9 AE 0.1%) but to a lesser extent.…”

Section: Ischaemic Corementioning

confidence: 86%

“…In addition, this difference is increased in ischaemic conditions, dropping to 0.51 AE 0.21 ml/g/min in the parietal cortex and 0.20 AE 0.15 ml/g/min in the temporal cortex. 29 Thirty minutes after MCAo, the absolute CBF in viable areas was 0.39 AE 0.15 ml/g/min, while in hypoxic brain tissue the CBF average was 0.30 AE 0.09 ml/g/min. 29 Hypoxic zones detected by the StO 2 (<40%) mapping could be compared with those detected by perfusion using a threshold.…”

Section: Study Limitationsmentioning

confidence: 92%

“…Engelhorn et al evaluated the absolute CBF using radioactivelabelled microspheres. 29 They demonstrated that the baseline CBF (before MCAo) is variable by region, for example between the parietal (0.88 AE 0.26 ml/g/ min) and temporal cortex (0.74 AE 0.24 ml/g/min). In addition, this difference is increased in ischaemic conditions, dropping to 0.51 AE 0.21 ml/g/min in the parietal cortex and 0.20 AE 0.15 ml/g/min in the temporal cortex.…”

Section: Study Limitationsmentioning

confidence: 99%

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Multiparametric magnetic resonance imaging including oxygenation mapping of experimental ischaemic stroke

Boisserand

Lemasson

Hirschler

et al. 2016

J Cereb Blood Flow Metab

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Recent advances in MRI methodology, such as microvascular and brain oxygenation (StO) imaging, may prove useful in obtaining information about the severity of the acute stroke. We assessed the potential of StO to detect the ischaemic core in the acute phase compared to apparent diffusion coefficient and to predict the final necrosis. Sprague-Dawley rats (n = 38) were imaged during acute stroke (D0) and 21 days after (D21). A multiparametric MRI protocol was performed at 4.7T to characterize brain damage within three region of interest: 'LesionD0' (diffusion), 'Mismatch' representing penumbra (perfusion/diffusion) and 'Hypoxia' (voxels < 40% of StO within the region of interest LesionD0). Voxel-based analysis of stroke revealed heterogeneity of the region of interest LesionD0, which included voxels with different degrees of oxygenation decrease. This finding was supported by a dramatic decrease of vascular and perfusion parameters within the region of interest hypoxia. This zone presented the lowest values of almost all parameters analysed, indicating a higher severity. Our study demonstrates the potential of StO magnetic resonance imaging to more accurately detect the ischaemic core without the inclusion of any reversible ischaemic damage. Our follow-up study indicates that apparent diffusion coefficient imaging overestimated the final necrosis while StO imaging did not.

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Does a relative perfusion measure predict cerebral infarct size?

Cited by 6 publications

References 17 publications

Predictable ventricular shift after focal cerebral ischaemia in rats: practical considerations for intraventricular therapeutic interventions

Predictable ventricular shift after focal cerebral ischaemia in rats: practical considerations for intraventricular therapeutic interventions

MicroRNA Expression in the Blood and Brain of Rats Subjected to Transient Focal Ischemia by Middle Cerebral Artery Occlusion

Multiparametric magnetic resonance imaging including oxygenation mapping of experimental ischaemic stroke

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