The issue of sustainable development is critical in globalization, the rapid growth of the industry, technological progress, and climate change. Sustainable development is the realization of economic, social, and environmental goals. It requires all actors of socioeconomic life, including enterprises, organizations, institutions, and countries. Sustainable development of an enterprise means improving the financial and property situation while taking care of employees and local communities' health and development and taking action to protect the natural environment. This paper aims to assess the impact of macroeconomic conditions on the sustainable development of transport enterprises in Central and Eastern European Countries (CEECs) in the period from 2008 to 2018. The selection of transport enterprises for the research sample is related to their high impact on economies in the analyzed countries, their significant negative impact on the natural environment. Moreover, transport enterprises play a vital role in the development of other economic sectors. The authors discuss the fundamental theoretical issues related to enterprises' sustainable development and the research result conducted in CEECs. The study results indicate a statistically significant relationship between the indicator of sustainable development and the hand of the macroeconomic condition in all analyzed countries. The highest correlation was observed in Romania, Hungary, and Slovenia, while the lowest in Croatia. The story of socio-economic development in surveyed countries plays a fundamental role in achieving sustainable development of transport enterprises.