Does Bone Marrow–derived Mesenchymal Stem Cell Transfusion Prevent Antisperm Antibody Production After Traumatic Testis Rupture?

Aghamir, Seyed Mohammad Kazem; Salavati, Alborz; Yousefie, Reza; Tootian, Zahra; Ghazaleh, Noushin; Jamali, Mostafa; Azimi, Pourya

doi:10.1016/j.urology.2014.03.009

Cited by 34 publications

(18 citation statements)

References 27 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…The last point is related to the BM-MSCs character, which is shown to be not only hypo-immunogenic but also to have immune-surveillance or immunosuppressive properties upon transplantation, and to be a good candidate for allogeneic transplantation (AI et al, 2012). Interestingly, intravenous transfusion of BM-MSCs was demonstrated to have immunomodulatory effects for the production of antisperm antibodies in allogeneic transplantations in mice after testicular ruptures (AGHAMIR et al, 2014). Therefore, allotransplantation of MSCs may be considered as the method of choice for azoospermia cell therapy.…”

Section: Discussionmentioning

confidence: 99%

Induction of spermatogenesis after stem cell therapy of azoospermic guinea pigs

et al. 2017

View full text Add to dashboard Cite

Bone marrow-derived mesenchymal stem cells (BM-MSCs) provide a large quantitative alternative source for regenerative medicine. This study was undertaken to determine the effect of BM-MSCs in the treatment of busulfan-induced azoospermia in guinea pigs. BM-MSCs were isolated from the femur bones of 6 adult guinea pigs as the donor group, and characterized by morphology, MSC markers and osteogenic and adipogenic differentiation. A dose of 40 mg/kg of busulfan was administered at a 21 day interval to induce azoospermia in 6 guinea pigs. Thirty-five days after the second injection of busulfan, transplantation of 1×10 6 BM-MSCs was performed into the seminiferous tubules of the left testes. The right testis was considered as the positive busulfan treated control. The testes of the donor group were applied as an intact normal control. Then, 60 days after cell therapy, histopathological and histomorphometric evaluations of the testes were performed. The seminiferous tubules treated with BM-MSCs, similar to the intact group, showed a normal appearance of spermatogenesis in comparison to the busulfan-induced azoospermic testes. In conclusion, BM-MSCs were effective in the treatment of azoospermia in a guinea pig model where they restore the fertility of busulfaninduced azoospermic animals after transplantation of BM-MSCs. Therefore, this report could open a window in future to the possibility of BM-MSCs transplantation in the treatment of azoospermia in humans, but more studies should be undertaken for further verification.

show abstract

Section: Discussionmentioning

confidence: 99%

Induction of spermatogenesis after stem cell therapy of azoospermic guinea pigs

et al. 2017

View full text Add to dashboard Cite

show abstract

“…Interestingly, BM-MSCs are not only hypo-immunogenic but also produce immunosurveillance or immunosuppression upon transplantation, therefore they are suitable for allogeneic transplantation ( 25 ). Intravenously bone BM-MSCs transfusion showed immunomodulatory effects on antisperm antibody production in allogeneic settings in mice after testis rupture ( 26 ). Moreover, sertoli cells are immune tolerant cells ( 27 ) and they can cause survival of the donor BM-MSCs and protect the transplanted allogeneic cells from post transplantation immune or inflammatory reaction.…”

Section: Discussionmentioning

confidence: 99%

Induction of Spermatogenesis by Bone Marrow-derived Mesenchymal Stem Cells in Busulfan-induced Azoospermia in Hamste

Tamadon

Mehrabani

Rahmanifar

et al. 2015

IJSC

View full text Add to dashboard Cite

BackgroundBone marrow-derived mesenchymal stem cells (BM-MSCs) have potential of differentiation and they secrete anti-inflammatory cytokines and growth factors which make them appropriate for cell therapy.Aim of the WorkWere to evaluate the healing effect of BM-MSCs transplantation on germinal cells of busulfan-induced azoospermic hamsters.Material and MethodsIn the present experimental case control study, BM-MSCs were isolated from bone marrow of donor albino hamsters. Five mature male recipient hamsters received two doses of 10 mg/kg of busulfan with 21 days interval to stop endogenous spermatogenesis. After induction of azoospermia, right testis of hamsters was injected with 106 BM-MSCs via efferent duct and the left one remained as azoospermia control testis. Five normal mature hamsters were selected as normal intact control. After 35 days, testes and epididymis of three groups were removed for histological evaluation.ResultsHistomorphological analyses of BM-MSCs treated testes and epididymis showed the epithelial tissue of seminiferous tubules had normal morphology and spermatozoa were present in epididymis tubes. Spermatogenesis was observed in most cell-treated seminiferous tubules. The untreated seminiferous tubules were empty.ConclusionTransplanted BM-MSCs could successfully induce spermatogenesis in seminiferous tubules of azoospermic hamster. Therefore, BM-MSCs can be an attractive candidate in cell transplantation of azoospermia.

show abstract

“…Nevertheless, mesenchymal stem cells generate immunosuppression or immunosurveillance upon transplantation [48]. It is interesting to mention that, related to the treatment of azoospermia, IV allogenic bone marrow-derived mesenchymal stem cell transfusion encourages the production of antisperm antibody modulated by immune system, after testis rupture in mice [49], which illustrates the other therapeutic potential of mesenchymal stem cells in the treatment of infertility. In fact, the possible mechanisms of azoospermia healing by mesenchymal stem cells are unclear; three main mechanisms could be responsible for the recovering of testicular function during the tissue regeneration process period by mesenchymal stem cells.…”

Section: Treatment Of Male Infertility By Msc Transplantationmentioning

confidence: 99%

Mesenchymal Stem Cell Therapy of Male Infertility

Tamadon¹,

Zhanbyrbekuly²,

Kairgaliyev³

et al. 2020

Male Reproductive Health

View full text Add to dashboard Cite

Nowadays mesenchymal stem cell (MSC) therapy offers a broad spectrum of treatment of different conditions, including male infertility. Lots of studies suggest that injection of MSC promotes differentiation of germ cells and/or stimulates gonadal tissue development. Currently, there are plenty of MSC therapies of azoospermia which have been studied on animal models and demonstrated good results. Most of the studies were conducted using MSC derived from adipose tissue, the bone marrow, and umbilical cord. Despite the fact that this type of treatment in humans is not established as a first choice option, the use of these techniques gives us hope for a gradual introduction into our daily practice.

show abstract

Does Bone Marrow–derived Mesenchymal Stem Cell Transfusion Prevent Antisperm Antibody Production After Traumatic Testis Rupture?

Cited by 34 publications

References 27 publications

Induction of spermatogenesis after stem cell therapy of azoospermic guinea pigs

Induction of spermatogenesis after stem cell therapy of azoospermic guinea pigs

Induction of Spermatogenesis by Bone Marrow-derived Mesenchymal Stem Cells in Busulfan-induced Azoospermia in Hamste

Mesenchymal Stem Cell Therapy of Male Infertility

Contact Info

Product

Resources

About