Does Cigarette Smoking Induce Changes in Biologic and Mechanical Properties of Platelet-Rich Fibrin Membranes?

Sridharan, Srirangarajan; Sindhu, Vanama; Prabhu, Srikumar; Rao, Ravi J; Rudresh, Vinaya

doi:10.11607/prd.4573

Cited by 7 publications

(7 citation statements)

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“…Our results agree with recent reports indicating similar levels of growth factors (EGF, FGF‐2, HGF, PDGF‐AA, TGD‐a, VEGF) in saliva, crevicular fluid or serum from smokers and nonsmokers 25,26 . As same, a recent report evidenced similar levels of PDGF‐AA and VEGF in L‐PRF membranes after blood sampling in smokers and nonsmokers without significant differences 27 …”

Section: Discussionsupporting

confidence: 93%

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Smoking habits do not affect biological responses induced by leucocyte and platelet‐rich fibrin in periodontal ligament cells

Rı́os

Álvarez

et al. 2022

J Oral Pathology Medicine

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Background and Objective Leucocyte‐ and platelet‐rich fibrin has been developed to stimulate wound healing response. However, it is currently unknown whether smoking affects the biological responses elicited by leucocyte‐ and platelet‐rich fibrin on periodontal ligament‐derived mesenchymal stromal cells. This study analyzes the kinetics of biomolecule release from leucocyte‐ and platelet‐rich fibrin derived from smokers and nonsmokers and their effect on periodontal ligament cell proliferation and migration as essential biological activities during wound healing. Methods Biomolecules present in leucocyte‐ and platelet‐rich fibrin exudates and conditioned media collected from smokers and nonsmokers were analyzed by Luminex arrays. Periodontal ligament‐derived mesenchymal stromal cell obtained from one nonsmoker were treated with leucocyte‐ and platelet‐rich fibrin exudates or leucocyte‐ and platelet‐rich fibrin conditioned media derived from both smokers and nonsmokers. The parameters evaluated included cell proliferation, determined by Ki67 immunostaining and migration assessed using transwell assays. Also, cells were treated with nicotine in the presence of fetal bovine serum 10% or leucocyte‐ and platelet‐rich fibrin conditioned media. Results A similar biomolecular profile was detected in leucocyte‐ and platelet‐rich fibrin exudates and leucocyte‐ and platelet‐rich fibrin conditioned media from smokers and nonsmokers, stimulating (periodontal ligament‐derived mesenchymal stromal cell) proliferation, and migration to a comparable degree. Nicotine reduced cell proliferation and migration of periodontal cells; however, this effect was recovered in the presence of leucocyte‐ and platelet‐rich fibrin conditioned media. Conclusion Leucocyte‐ and platelet‐rich fibrin derived from smokers could be an autologous source of biomolecules to stimulate cell biological activities involved in wound healing in smokers who have difficulties in ceasing this habit. Clinical trials are required to evaluate the impact of leucocyte‐ and platelet‐rich fibrin on healing responses in smokers.

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Section: Discussionsupporting

confidence: 93%

“…25,26 As same, a recent report evidenced similar levels of PDGF-AA and VEGF in L-PRF membranes after blood sampling in smokers and nonsmokers without significant differences. 27 We mostly did not find significant differences in the proliferation of cells treated with LPRF-CM derived from smokers and nonsmokers.…”

Section: Discussionmentioning

confidence: 57%

Smoking habits do not affect biological responses induced by leucocyte and platelet‐rich fibrin in periodontal ligament cells

Rı́os

Álvarez

et al. 2022

J Oral Pathology Medicine

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“…Therefore, we excluded chronic smokers from our study protocol. 29 Additionally, systemically compromised patients, such as those with bleeding disorders, diabetes mellitus, or patients on drug therapy that might affect the outcomes of the study were excluded.…”

Section: Discussionmentioning

confidence: 99%

Comparison of the angiogenic efficacy of conventional leukocyte- and platelet-rich fibrin versus low-speed advanced platelet-rich fibrin: An in vitro chorioallantoic membrane assay study

Nair,

Thomas,

Shah

et al. 2024

Dent. Med. Probl.

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Background. Platelet-rich fibrin (PRF) is widely used in periodontics for its wound healing potential. Two major variations of PRF are the original leukocyte-and platelet-rich fibrin (L-PRF) and the modified lowspeed advanced PRF (A-PRF).Objectives. The aim of the present study was to evaluate and compare the conventional L-PRF protocol and the low-speed A-PRF protocol in terms of angiogenic potential of PRF, using an in vivo chorioallantoic membrane (CAM) assay. Material and methods.Fifteen fertile Giriraja eggs were procured and after a 3-day incubation period, randomly allotted into 3 groups: control; L-PRF; and A-PRF. A total of 20 mL of blood was collected from systemically healthy male volunteers aged 18-24 years, using a standard protocol. The PRF samples were inoculated on the CAM of the eggs. On the 10th day, the eggs were reopened and photographed. The parameters assessed were the number, length, size, and density of blood vessels, as well as the number of junctions formed. The photographs were analyzed using the ImageJ and ProgRes ® CapturePro software.Results. Seven days after inoculation, both the A-PRF and L-PRF groups exhibited significantly better results than the control group in terms of the number (

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“…The negative effect of smoking is widely known, in particular after periodontal regeneration techniques (54). Although a direct correlation between smoking and the quality of platelet concentrates is yet to be established, evidence suggests that smoking has the potential to influence mechanical and biological effect of PRF materials (55). However, there is currently a lack of extensive literature on this topic.…”

Section: Discussionmentioning

confidence: 99%

Effects of Advanced Platelet Rich Fibrin (A-PRF+), Enamel Matrix Derivative (EMD) and Open Flap Debridement on clinical and wound healing parameters in molar furcation sites: A case series from a RCT study

Pitzurra,

Vasdravellis,

Rosema

et al. 2023

Front. Dent. Med

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AimTo study effects of advanced platelet rich Fibrin (A-PRF+) and enamel matrix derivative (EMD) compared to open flap debridement (OFD) alone in molar furcation sites grade II on clinical and wound healing parameters.Materials and methodsA randomized controlled trial was designed. Eligible patients were randomly allocated in one of three treatment groups: A-PRF+, EMD or OFD. The patients and clinical examiners were blinded for the treatment received. A minimally invasive microsurgical approach was performed for the three modalities. Clinical measurements were scored at baseline and 6 months postoperatively. The clinical healing of each furcation was scored via Early Wound Healing Index on day 3, 1 week, 2 weeks and 6 weeks.Results17 patients (A-PRF+ n = 6, EMD n = 5, OFD n = 6) completed the 6 months follow-up. The further completion of the trial had to be cancelled due to the COVID-19 pandemic. In three patients in the A-PRF+ group, the grade II of the treated furcation regressed to grade I; the corresponding number in the EMD and OFD groups were zero and one respectively. Further, 3, 1 and 4 patients in the PRF, EMD and OFD groups respectively, showed a gain of bone level ≥1 mm. The defects in the A-PRF+ group showed delayed early healing compared to EMD and OFD groups.ConclusionThe case series (RCT design) suggest a slight advantage for A-PRF+ over EMD and OFD, regarding the regressing of a furcation II to grade I, however this treatment showed delayed early wound healing compared to EMD or OFD.Clinical Trial Registrationhttps://www.isrctn.com/, identifier ISRCTN13520922.

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Does Cigarette Smoking Induce Changes in Biologic and Mechanical Properties of Platelet-Rich Fibrin Membranes?

Cited by 7 publications

References 0 publications

Smoking habits do not affect biological responses induced by leucocyte and platelet‐rich fibrin in periodontal ligament cells

Smoking habits do not affect biological responses induced by leucocyte and platelet‐rich fibrin in periodontal ligament cells

Comparison of the angiogenic efficacy of conventional leukocyte- and platelet-rich fibrin versus low-speed advanced platelet-rich fibrin: An in vitro chorioallantoic membrane assay study

Effects of Advanced Platelet Rich Fibrin (A-PRF+), Enamel Matrix Derivative (EMD) and Open Flap Debridement on clinical and wound healing parameters in molar furcation sites: A case series from a RCT study

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