Organic agriculture is growing in importance to address environmental challenges and meet the rising consumer demand for healthy, eco-friendly agricultural products. However, the success and growth of organic rice agribusiness depend heavily on the active involvement and collaboration of stakeholders across the entire agricultural value chain. Therefore, the present research aims to analyze the pivotal role of stakeholders in the development of organic rice agribusiness in the Karanganyar Regency. To achieve this objective, a qualitative approach was used to collect data through in-depth interviews, participatory observation, and Focus Group Discussions with stakeholders such as farmers, business actors, local government, local communities, and consumers. The collected data were then analyzed using stakeholders’ analysis and thematic approaches to identify the roles, contributions, and challenges faced by each stakeholder. The results showed that stakeholders play a crucial role in the development of organic rice agribusiness. Farmers have a central role as primary producers and holders of local knowledge about organic farming practices. Additionally, business actors contribute by facilitating product marketing, processing, and distribution. Local governments are instrumental in providing policy and infrastructure support while also facilitating training and assistance for farmers. To ensure the success and growth of organic rice agribusiness, collaboration and synergy among stakeholders are vital as they establish networks, expand markets, access funding, and deliver education and training programs to farmers.