Does Botrytis cinerea Ignore H2O2-Induced Oxidative Stress During Infection? Characterization of Botrytis Activator Protein 1

Temme, Nora; Tudzynski, Paul

doi:10.1094/mpmi-22-8-0987

Cited by 146 publications

(153 citation statements)

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“…E. festucae YapA functionally complemented the H 2 O 2 stress sensitivity defect of an S. cerevisiae ⌬yap1 mutant and translocated to the nuclei of E. festucae cells in response to H 2 O 2 , properties that demonstrate that YapA is capable of activating genes required for an H 2 O 2 -induced oxidative stress response in S. cerevisiae. However, unlike other filamentous fungi (8,32,33,58), the E. festucae ⌬yapA mutants did not show increased hyphal sensitivity to H 2 O 2 compared to the wild type. Hyphae of the E. festucae ⌬yapA mutants were also resistant to KO 2 and t-BOOH but very sensitive to menadione and slightly sensitive to diamide.…”

Section: Discussionmentioning

confidence: 64%

“…The assumption has been that the immediate sensor of increased levels of H 2 O 2 would be a thiol peroxidase (7,33), as has been established for the well-characterized Yap1-Gpx3 and Pap1-Tpx1 redox relay systems that operate in the yeast fungi S. cerevisiae and S. pombe, respectively (21,24,25). The abundance of these proteins in the cell and their favorable kinetic properties compared to those of alternative thiol-reactive proteins support this hypothesis (69).…”

Section: Figmentioning

confidence: 95%

“…Although AP-1 transcription factors have been characterized in a range of filamentous fungi, the mechanism by which oxidative stress is sensed and transduced to the AP-1 protein is still not known (8,(30)(31)(32)(33)(34). The assumption has been that the immediate sensor of increased levels of H 2 O 2 would be a thiol peroxidase (7,33), as has been established for the well-characterized Yap1-Gpx3 and Pap1-Tpx1 redox relay systems that operate in the yeast fungi S. cerevisiae and S. pombe, respectively (21,24,25).…”

Section: Figmentioning

confidence: 99%

“…Yap1 homologs have been identified in a number of other fungi, including Candida albicans (29), Aspergillus fumigatus (30), Cochliobolus heterostrophus (31), Alternaria alternata (32), Botrytis cinerea (33), Neurospora crassa (34), Magnaporthe oryzae (8), and Ustilago maydis (7). Disruption of these transcription factors commonly results in hyphal and conidial sensitivity to H 2 O 2 and other oxidative stress-inducing compounds, such as tert-butyl hydroperoxide (t-BOOH), potassium superoxide (KO 2 ), menadione, and diamide.…”

mentioning

confidence: 99%

“…AP-1 mutants of the biotrophic fungi U. maydis and M. oryzae are sensitive to a host oxidative burst and have reduced virulence (7,8). In contrast, the AP-1 mutants of the necrotrophic fungi B. cinerea and C. heterostrophus are as virulent as the wildtype strains (31,33). A. alternata is an exception to the generalization that lifestyle determines the host colonization outcome, as AP-1 mutants of this necrotrophic pathogen are defective in host colonization (32).…”

mentioning

confidence: 99%

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Redox Regulation of an AP-1-Like Transcription Factor, YapA, in the Fungal Symbiont Epichloë festucae

Cartwright

Scott

2013

Eukaryot Cell

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One of the central regulators of oxidative stress in Saccharomyces cerevisiae is Yap1, a bZIP transcription factor of the AP-1 family. In unstressed cells, Yap1 is reduced and cytoplasmic, but in response to oxidative stress, it becomes oxidized and accumulates in the nucleus. To date, there have been no reports on the role of AP-1-like transcription factors in symbiotic fungi. An ortholog of Yap1, named YapA, was identified in the genome of the grass symbiont Epichloë festucae and shown to complement an S. cerevisiae ⌬yap1 mutant. Hyphae of the E. festucae ⌬yapA strain were sensitive to menadione and diamide but resistant to H 2 O 2 , KO 2 , and tert-butyl hydroperoxide (t-BOOH). In contrast, conidia of the ⌬yapA strain were very sensitive to H 2 O 2 and failed to germinate. Using a PcatA-eGFP degron-tagged reporter, YapA was shown to be required for expression of a spore-specific catalase gene, catA. Although YapA-EGFP localized to the nucleus in response to host reactive oxygen species during seedling infection, there was no difference in whole-plant and cellular phenotypes of plants infected with the ⌬yapA strain compared to the wild-type strain. Homologs of the S. cerevisiae and Schizosaccharomyces pombe redox-sensing proteins (Gpx3 and Tpx1, respectively) did not act as redox sensors for YapA in E. festucae. In response to oxidative stress, YapA-EGFP localized to the nuclei of E. festucae ⌬gpxC, ⌬tpxA, and ⌬gpxC ⌬tpxA cells to the same degree as that in wild-type cells. These results show that E. festucae has a robust system for countering oxidative stress in culture and in planta but that Gpx3-or Tpx1-like thiol peroxidases are dispensable for activation of YapA.

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Section: Discussionmentioning

confidence: 64%

Section: Figmentioning

confidence: 95%

Section: Figmentioning

confidence: 99%

mentioning

confidence: 99%

mentioning

confidence: 99%

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Redox Regulation of an AP-1-Like Transcription Factor, YapA, in the Fungal Symbiont Epichloë festucae

Cartwright

Scott

2013

Eukaryot Cell

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Biocontrol yeast T‐2 improves the postharvest disease resistance of grape by stimulation of the antioxidant system

Wang

et al. 2022

Food Science & Nutrition

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As independent individuals, the postharvest grapefruits will undergo a series of physiological and biochemical changes when dealing with pathogenic bacteria. Reactive oxygen species (ROS) are reactive molecules and free radicals produced by oxygen molecules, and its production is an initial and universal response of plant to pathogen attack (Mittler, 2002;. Uncontrolled overproduction of ROS disrupts normal metabolism by oxidizing proteins and lipids affecting the integrity of cell membranes and inactivating cellular functions (Xu et al., 2008). The gray mold caused by Botrytis cinerea (B. cinerea) is one of the most widely postharvest diseases in grapes. Considerable researches show that B. cinerea can initiate

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Botrytis cinerea differentially induces postharvest antioxidant responses in ‘Braeburn’ and ‘Golden Delicious’ apple fruit

Bui

Wright

Falk³

et al. 2019

J Sci Food Agric

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BACKGROUND The fruit of two apple cultivars – ‘Braeburn’, which is susceptible to inoculation with Botrytis cinerea, and the less susceptible cv. ‘Golden Delicious’ – were investigated with respect to their response to inoculation with B. cinerea. Successful infection by B. cinerea leads to an oxidative burst and perturbation of plant redox homeostasis. To investigate the interaction between apple fruit and B. cinerea, antioxidant metabolism in fruit samples from sun‐exposed and shaded sides of different tissue types was measured over time. RESULTS The sun‐exposed tissue of ‘Braeburn’ had higher initial levels of total vitamin C in the peel and phenolic compounds in the flesh than ‘Golden Delicious’, despite its greater susceptibility to gray mold. A substantial antioxidant response was recorded in diseased ‘Braeburn’ fruit 14 days after inoculation, which involved an elevated superoxide dismutase activity and ascorbate peroxidase activity, a progressive oxidation of total vitamin C, and a decrease in peroxidase activity and phenolic content. Disease development was slower on the sun‐exposed sides than on the shaded sides. CONCLUSION The two cultivars appeared to utilize different strategies to defend themselves against B. cinerea. ‘Golden Delicious’ almost entirely escaped infection. Preharvest exposure of apple fruit to high light / temperature stress appears to prepare them to better resist subsequent postharvest attack and disease. © 2019 The Authors. Journal of The Science of Food and Agriculture published by John Wiley & Sons Ltd on behalf of Society of Chemical Industry.

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Does Botrytis cinerea Ignore H₂O₂-Induced Oxidative Stress During Infection? Characterization of Botrytis Activator Protein 1

Cited by 146 publications

References 47 publications

Redox Regulation of an AP-1-Like Transcription Factor, YapA, in the Fungal Symbiont Epichloë festucae

Redox Regulation of an AP-1-Like Transcription Factor, YapA, in the Fungal Symbiont Epichloë festucae

Biocontrol yeast T‐2 improves the postharvest disease resistance of grape by stimulation of the antioxidant system

Botrytis cinerea differentially induces postharvest antioxidant responses in ‘Braeburn’ and ‘Golden Delicious’ apple fruit

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