Does intraperitoneal insemination in the absence of prior sensitization carry with it a risk of subsequent immunity to sperm?

Livi, Claudia; Coccia, E.; Versari, Laura; Pratesi, Stefano; Buzzoni, P

doi:10.1016/s0015-0282(16)53229-4

Cited by 13 publications

(10 citation statements)

References 16 publications

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“…On the other hand, it is well known that the i.p. injection of ejaculated spermatozoa in women triggers adverse immune reaction against spermatozoa in only a few cases [23]. This fact can be due to specific seminal proteins inducing a decrease in the immunogenicity of the epididymal spermatozoa following ejaculation.…”

Section: Discussionmentioning

confidence: 99%

“…immunization model to mating derives from the following considerations: 1) both the peritoneal cavity and the female genital tract are fully immunocompetent anatomic environments [1,5,7]; 2) after mating, live spermatozoa are detectable in the peritoneal cavity [22]; and 3) i.p. injection of spermatozoa is frequently used in the medical practice of artificial insemination with a high degree of success and without immunologic side effects [23].…”

Section: Introductionmentioning

confidence: 99%

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Suppression of Rat Epididymal Sperm Immunogenicity by a Seminal Vesicle Secretory Protein and Transglutaminase Both in Vivo and in Vitro1

Peluso¹,

Porta²,

Esposito³

et al. 1994

Biology of Reproduction

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The pretreatment of epididymal spermatozoa with SV-IV, one of the major secretory protein produced by the epithelium of adult rat seminal vesicles, was found to markedly decrease their ability to induce in vivo peritoneal macrophage activation, measured as class II major histocompatibility complex surface antigen expression, superoxide anion production, phagocytic activity, and antigen presentation. In addition, the treatment of spermatozoa with SV-IV produced a significant decrease of their immunogenicity evaluated in vitro by [3H]thymidine incorporation in splenocyte/spermatozoon co-culture. The concurrent presence of SV-IV and transglutaminase, an enzyme secreted in large amounts from the rat anterior prostate, amplified these phenomena. The suppression of the epididymal sperm immunogenicity is suggested to be of crucial importance for the prevention of the immune response to the sperm introduced in the immunocompetent female genital tract during coitus.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Suppression of Rat Epididymal Sperm Immunogenicity by a Seminal Vesicle Secretory Protein and Transglutaminase Both in Vivo and in Vitro1

Peluso¹,

Porta²,

Esposito³

et al. 1994

Biology of Reproduction

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“…In females mechanical or chemical disruption of the mucosal layer of the female genital tract may permit exposure to foreign sperm antigens and ultimately formation of ASA 5 . Women undergoing repeated intra uterine insemination (IUI) with washed spermatozoa, are also thought to be prone to develop ASA 6 . Removal of immnosuppressive properties of seminal plasma when preparing the semen for IUI could be an added factor 7 .…”

Section: Introductionmentioning

confidence: 99%

Antisperm antibodies: Incidence, isotypes and location on spermatozoa, their implications on fertilization and on pregnancy rate at a selected centre in Sri Lanka

Tennakoon¹,

Yasawardene

Weerasekera³

2012

Sri Lanka J Obstet & Gynae

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Objectives: 1. To investigate the incidence, isotypes and location of antisperm antibodies (ASA) on the sperm surface in an infertile population. 2. To study the influence of isotypes, location of ASA on the sperm surface on fertilization, cleavage rates of embryos and pregnancy rate. Methods:A prospective analytical study was carried out on infertile couples who underwent Assisted Reproductive Technology (ART) procedures at 'Prarthana' Centre for in vitro fertilization (IVF) during three year period from 01.01.2006 -01.01.2009. Presence of ASA was elicited using mixed agglutination reaction latex bead test (Sperm MAR, Fertipro NV, Belgium) in male and female subjects. The isotype and location of ASA, detected by the type (i.e. IgA, IgG) and the site of binding of latex beads to ASA on the spermatozoa (i.e. head, midpiece, tail of the sperm) was analyzed. In females who underwent In-vitro fertilization, fertilization rate and day 03 cleavage rate of embryos were assessed. The pregnancy rates following each ART procedure were noted. A total of 230 infertile couples were investigated.Results: Out of 230 couples, 48 (20.86%) were positive for ASA. Researchrespectively. The total fertilization rate and cleavage rate among ASA positives were 69.38% (179/258) and 53.63% (96/179) respectively; and among ASA negatives the rates were 58.54% (473/808) and 61.73% (292/473) respectively. Couples with IgA, IgG and IgA+IgG achieved a pregnancy rate of 10.53%, 23.08% and 18.75% respectively. Out of 08 pregnancies 05, 02, 01 had ASA on tail, midpiece+tail and whole sperm respectively. Conclusions:It was found that presence of ASA has negative effects on fertilization and post fertilization events. IgA isotype of ASA demonstrated the lowest pregnancy rate. When majority of sperms are coated with ASA on the heads or mid piece+tail, cleavage rates were often impaired. Intra-cytoplasmic sperm injection is recommended for such couples to achieve a successful pregnancy.

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“…It is not easy to say what starts the immunological reaction. This reaction has been reported to occur even after a single insemination (Critser et al, 1989;Livi et al, 1990). The large number of spermatozoa injected in the peritoneal cavity could explain this finding.…”

Section: Discussionmentioning

confidence: 81%

Lack of immunization after intraperitoneal insemination of spermatozoa

et al. 2009

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Summary. Twenty infertile patients with normal tubal patency were inseminated intraperitoneally (11 once, seven twice, and two three times) with spermatozoa (mean 14 times 106, range 0.6–48 times 106) prepared by the standard swim‐up technique. The occurrence of immunization to spermatozoa was looked for by the Gelatin Agglutination Test (GAT) and Tray Agglutination Test (TAT). Both tests gave negative results for all the controls (10 pregnant and 10 puerperal women). Antisperm antibodies were measured in the serum before, 30 d and 4–7 months after Intraperitoneal Insemination (IPI). The last check was done for only 14 patients, since six became pregnant as a consequence of the first treatment. Of the 14 patients studied after 4–7 months, seven had two, and two had three IPI. In the group of inseminated patients, 18 women with no basal sperm antibody did not show evidence of antibody formation after the treatment and it was not increased after insemination in the two patients who already had low antibody titre (1/32). In conclusion, despite the large number of spermatozoa inseminated and even after several IPI attempts, there was no evidence of de novo production of or increase in already present anti‐sperm antibodies according to the methods used for the detection of ASA in this study.

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Does intraperitoneal insemination in the absence of prior sensitization carry with it a risk of subsequent immunity to sperm?

Cited by 13 publications

References 16 publications

Suppression of Rat Epididymal Sperm Immunogenicity by a Seminal Vesicle Secretory Protein and Transglutaminase Both in Vivo and in Vitro1

Suppression of Rat Epididymal Sperm Immunogenicity by a Seminal Vesicle Secretory Protein and Transglutaminase Both in Vivo and in Vitro1

Antisperm antibodies: Incidence, isotypes and location on spermatozoa, their implications on fertilization and on pregnancy rate at a selected centre in Sri Lanka

Lack of immunization after intraperitoneal insemination of spermatozoa

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