Does leukocytospermia associate with poor semen parameters and sperm functions in male infertility?

Kaleli, Semih; Öçer, Fahri; İrez, Tülay; Budak, Erdal; Aksu, M. Feridun

doi:10.1016/s0301-2115(99)00204-3

Cited by 57 publications

(34 citation statements)

References 32 publications

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“…Lewis and Aitken (2005) demonstrated that DNA damage will decrease in vivo and in vitro fertilization rates [35], and presence of excess leukocytes is cited as a cause for DNA fragmentation, but other authors suggest that leukocytospermia is not a cause of decreased in vitro fertilization results [36,37].…”

Section: Discussionmentioning

confidence: 99%

Effect of leukocytospermia and processing by discontinuous density gradient on sperm nuclear DNA fragmentation and mitochondrial activity

Fariello

Giudice

Spaine

et al. 2009

J Assist Reprod Genet

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Purpose To assess the effect of leukocytospermia and semen processing on sperm DNA and mitochondria. Methods Twenty-two patients with and 41 without leukocytospermia were included. Sperm DNA fragmentation was assessed by the Comet assay, and mitochondrial activity by a colorimetric method for active mitochondria. Semen was processed using Percoll, and motility, DNA fragmentation, and mitochondrial activity were analyzed pre-and post-processing. Results No differences were observed in age, abstinence, volume, sperm morphology, progressive motility, concentration, and vitality (p>0.10). Variables were grouped according to time (pre-vs post-processing) and group (leukocytospermia vs non-leukocytospermia) because no interactions could be observed. Leukocytospermia was associated to increased DNA fragmentation, while semen processing led to a decrease in DNA fragmentation and to increased mitochondrial activity. Conclusion While semen processing selects sperm with higher rates of DNA integrity independent of the presence or absence of leukocytes in semen, samples without leukocytospermia present more sperm without DNA fragmentation. Semen processing also selects sperm with higher mitochondrial activity.

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Section: Discussionmentioning

confidence: 99%

Effect of leukocytospermia and processing by discontinuous density gradient on sperm nuclear DNA fragmentation and mitochondrial activity

Fariello

Giudice

Spaine

et al. 2009

J Assist Reprod Genet

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“…Eggert-Kruse et al [13] did not find any significant association between leukocytospermia and the production of IgA and IgG antisperm antibodies in semen. Kaleli et al [31] found a significant positive correlation between increased leukocyte counts and increased hypo-osmotic swelling test scores, higher sperm concentrations, and enhanced acrosome reactions. Kiessling et al [4] found higher frequencies of spermatozoa with normal morphology in the presence of leukocytospermia.…”

Section: Effect Of Leukocytospermia On Semen Parameters and Sperm Funmentioning

confidence: 97%

Leukocytospermia

Menkveld

2004

International Congress Series

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The role of leukocytes in semen (leukocytospermia) is still controversial. Contradictory reports on leukocytospermia have been published indicating negative effects on semen parameters and even in vitro fertilization (IVF) results, while other investigators did not find any negative influences of leukocytospermia on semen parameters. A few papers indicated a positive role for seminal leukocytes by elimination of morphological abnormal spermatozoa by phagocytosis. However, from the most recent literature, it is now clear that the most important deleterious effect of leukocytospermia may be the production of reactive oxygen species (ROS) causing DNA fragmentation of spermatozoa, leading to significant reduced pregnancy rates with IVF and intracytoplasmic sperm injection (ICSI) when oocytes are inseminated with spermatozoa from leukocytospermic semen samples. Contradictive results on treatment of leukocytospermia by antibiotics have also been published. However, a survey of the literature indicated that more evidence is presented testifying that antibiotic treatment of leukocytospermia may be beneficial by lowering the number of seminal leukocytes and even improving semen parameters. Therefore, treating leukocytospermia seems indicated. D 2004 Published by Elsevier B.V.

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“…Leukocytospermia A high concentration of WBCs (≥1 × 10 6 /mL) in semen samples is a marker of microbial inflammation (54). Male accessory gland infections which produced leukocytes was a condition frequently detected in infertile patients (55,56). Leukocytes appear in semen as the addition to bacteriospermia at the second stage of the UTI, and remain present in semen for some period of time following the elimination of the bacteria in the third stage (1).…”

Section: Male Genital Tract Infection and Bacteriospermiamentioning

confidence: 99%