Does macrovesicular endocytosis occur in fungal hyphae?

Mazheika, I. S.; Камзолкина, О. В.

doi:10.1016/j.fbr.2021.07.001

Cited by 4 publications

(10 citation statements)

References 60 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In our previous works (Mazheika et al, 2020b; Mazheika, Kamzolkina, 2021), studying static photographs of AM4-64 labeling taken in a single focal section in xylotrophs, we took glomeruli for primary endocytic invaginations/macrovesicles, pendants and small vesicles for endosomes, and large vesicles for vacuoles-lysosomes. The consistent appearance of the macroinvaginations in microscopic slides: glomeruli – small vesicles – and finally large vesicles – additionally confirmed that we were dealing with endocytic absorption of the styryl label, and was in good agreement with the endocytic pathways described in the literature in yeast and some filamentous fungi (Vida, Emr, 1995; Fischer-Parton et al, 2000; Peñalva, 2005).…”

Section: Discussionmentioning

confidence: 99%

“…Tubular invaginations (up to five µm long) at the fluorescent-microscopic level are described for filamentous basidiomycetes in our previous works (Mazheika et al, 2020a;Mazheika, Kamzolkina, 2021). At the electron-microscopic level, invaginating tubes are often presented as curved towards their base and are described not only in filamentous basidiomycetes, but also in yeasts (Smaczynska-de Rooij et al, 2011;Palmer et al, 2015;Schuster, Steinberg, 2020).…”

Section: Introductionmentioning

confidence: 93%

“…The plasma membrane (PM) of fungal cells is capable of forming large-sized invaginations exceeding 100 nm in diameter or length (Mazheika, Kamzolkina, 2021). There are three main types of such invaginations: macrovesicular invaginations, tubes and eizosomes.…”

Section: Introductionmentioning

confidence: 99%

“…Macroinvaginations III correspond at the electron-microscopic level to classical lomasomes (see below). Assumedly there is a relation between macroinvaginations II and III – it is possible that macroinvagination II, forming secondary invaginations, can form macroinvagination type III (Mazheika, Kamzolkina, 2021). It should be noted that in previous work, the term “macroinvagination” was used primarily to refer to the macrovesicular invaginations of the PM.…”

Section: Introductionmentioning

confidence: 99%

“…Classical lomasomes (for terminology see Mazheika, Kamzolkina, 2021) have been found in fungi for a long time, one of the first observations is found in the study of Girbardt (1958) on Polyporus versicolor . Most often, lomasomes are found in electron microscopic samples in basidial xylotrophs, but they are also found in other fungi and fungi-like organisms (Moore, McAlear, 1961; Heath, Greenwood, 1970; Brushaber, Jenkins, 1971; Marchant, Moore, 1973).…”

Section: Introductionmentioning

confidence: 99%

See 4 more Smart Citations

Lomasomes and other fungal plasma membrane macroinvaginations have a tubular and lamellar genesis

Mazheika

Камзолкина

2022

Preprint

Self Cite

View full text Add to dashboard Cite

The plasma membrane of filamentous fungi forms large invaginations (>100-200 nm in diameter or length), which are especially pronounced in xylotrophic basidiomycetes. Typically, such macroinvaginations are either tubules or parietal vesicles. Vesicular macroinvaginations at the electron microscopic level correspond to classical lomasomes. There is an assumption that vesicular macroinvaginations/lomasomes may be involved in macrovesicular endocytosis, i.e., be scissored from the plasma membrane and be transported to the cytoplasm. The original aim of this study was to test for the presence of macroendocytosis in xylotrophic fungi using a fluorescent styryl label and time-lapse and Z-stacks microscopic technologies. However, the results were unexpected. It was found that most of the membrane structures labeled in fungal cells by the styryl fluorophore (analogue of FM4-64) is various types of macroinvaginations of the plasma membrane. All of these macroinvaginations are or go through a tube or lamella stage. Moreover, under specific conditions of a microscopic preparation, different types of invaginations are formed at different times - the longer the sample observation time, the greater may be the diameter of the tubes that form macroinvaginations. In addition, the morphology and successive formation of macroinvaginations mimics the endocytic pathway: different types of the invaginations can easily be mistaken for primary endocytic vesicles, endosomes, and vacuole-lysosomes. The paper qualitatively and quantitatively analyzes various types of macroinvaginations of the plasma membrane, suggests their biological functions, and discusses the presence and features of classical and macrovesicular endocytosis in fungi. This study is another step towards understanding the complex physiology of filamentous fungi associated with changes in plasma membrane tension, rapid changes in hyphae size, and endocytosis. In addition, this work is a presentation of a new tubular system in wood-decaying fungi, which takes its place among the already described tubular systems: vacuolar, mitochondrial, and endoplasmic-reticular.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 93%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Lomasomes and other fungal plasma membrane macroinvaginations have a tubular and lamellar genesis

Mazheika

Камзолкина

2022

Preprint

Self Cite

View full text Add to dashboard Cite

show abstract

Instantaneous change in hyphal diameter in basidiomycete fungi

Mazheika,

Voronko,

Kolomiets

et al. 2024

Preprint

Self Cite

View full text Add to dashboard Cite

Under certain conditions, fungi are capable to fast (down to seconds) change the size of their cells. For example, it is known that the cells of many species of yeasts under hyperosmotic conditions instantly and reversibly shrink entirely, without plasmolysis, with a decrease in volume of up to 40% or more. There is limited data that filamentous fungi can also instantly change the diameter of their unspecialized hyphae. This property is fundamental and is a powerful tool for survival and preservation of the physiological functionality of the fungus in sharply changing external conditions, but requires detailed study. In this work, which is a large-scale (more than 50,000 cells measured) light microscopic study, the ability of three unrelated basidiomycete species to fast change the diameter of their hyphae under the influence of various factors was analyzed. It was found, for example, that all three fungi respond similarly to moderate hyperosmotic shock and shrink by an average of 12-14% in diameter, but inhibitors of actin assembly can cause both expansion and shrinkage of hyphae, or have no effect on a fungus. Combining the current results with those previously obtained in the study of macroinvagination systems of the fungal plasma membrane, different approaches for basidiomycetes to respond to sudden stress changes were proposed. The curtain model was also expanded, describing a common rapid response mechanism. The mechanism includes elastic properties of the cell wall, adhesion of the plasma membrane to it, regulation of turgor, the actin cables system that regulate the tension of the plasma membrane and cell size, and the complex system of macroinvaginations of the plasma membrane, ensuring the constant integrity and functionality of the latter.

show abstract

Lomasomes and Other Fungal Plasma Membrane Macroinvaginations Have a Tubular and Lamellar Genesis

Mazheika

Psurtseva

Камзолкина

2022

JoF

View full text Add to dashboard Cite

The plasma membrane of filamentous fungi forms large-sized invaginations, which are either tubes or parietal vesicles. Vesicular macroinvaginations at the ultrastructural level correspond to classical lomasomes. There is an assumption that vesicular macroinvaginations/lomasomes may be involved in macrovesicular endocytosis. The original aim of this study was to test for the presence of macroendocytosis in xylotrophic basidiomycetes using time-lapse and Z-stacks fluorescent microscopic technologies. However, the results were unexpected since most of the membrane structures labeled by the endocytic tracer (FM4-64 analog) are various types of plasma membrane macroinvaginations and not any endomembranes. All of these macroinvaginations have a tubular or lamellar genesis. Moreover, under specific conditions of a microscopic preparation, the diameter of the tubes forming the macroinvaginations increases with the time of the sample observation. In addition, the morphology and successive formation of the macroinvaginations mimic the endocytic pathway; these invaginations can easily be mistaken for endocytic vesicles, endosomes, and vacuole-lysosomes. The paper analyzes the various macroinvagination types, suggests their biological functions, and discusses some features of fungal endocytosis. This study is a next step toward understanding complex fungal physiology and is a presentation of a new intracellular tubular system in wood-decaying fungi.

show abstract

Does macrovesicular endocytosis occur in fungal hyphae?

Cited by 4 publications

References 60 publications

Lomasomes and other fungal plasma membrane macroinvaginations have a tubular and lamellar genesis

Lomasomes and other fungal plasma membrane macroinvaginations have a tubular and lamellar genesis

Instantaneous change in hyphal diameter in basidiomycete fungi

Lomasomes and Other Fungal Plasma Membrane Macroinvaginations Have a Tubular and Lamellar Genesis

Contact Info

Product

Resources

About