Does Needle Design Affect the Regenerative Potential of Bone Marrow Aspirate? An In Vitro Study

Feddahi, Nadia; Herten, Monika; Tassemeier, Tjark; Rekasi, Heike; Hackel, Alexander; Haversath, Marcel; Jäger, Markus

doi:10.3390/life11080748

Cited by 6 publications

(3 citation statements)

References 50 publications

(59 reference statements)

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“…In particular, the collection of larger bone marrow aspirate volumes with high volume syringes seem to decrease the concentration of BMSCs because of the dilution of the bone marrow sample with peripheral blood [ 49 , 49 ]. The needle used for bone marrow harvesting could be another factor affecting the quality of BMAC and its amount of BMSCs [ 21 , 40 , 42 , 58 ]. Some authors also suggested that a multiple-site harvest can be superior versus a single-site harvest [ 51 ].…”

Section: Discussionmentioning

confidence: 99%

Bone marrow aspirate concentrate quality is affected by age and harvest site

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Merli

et al. 2022

Knee Surg Sports Traumatol Arthrosc

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Purpose To compare the number and properties of bone marrow stromal cells (BMSCs) collected from bone marrow aspirate concentrate (BMAC) obtained from different harvest sites and from patients of different ages. Methods BMAC was obtained from two groups of patients based on age (n = 10 per group): 19.0 ± 2.7 years for the younger and 56.8 ± 12.5 for the older group. In the latter, BMAC was obtained from both iliac crest and proximal tibia for a donor-matched analysis. Mononucleated cell count and CFU-F assay were performed, together with phenotype characterization of BMSCs from iliac crest and proximal tibia, the study of chondrogenic and osteogenic differentiation capacity, histological staining and spectrophotometric quantification, and the analysis of mRNAs expression. Results Cells derived from iliac crest and proximal tibia showed the same phenotypic pattern at flow cytometry, as well as similar chondrogenic and osteogenic potential. However, a significantly higher number of mononuclear cells per ml was observed in younger patients (3.8 ± 1.8 × 107) compared to older patients (1.2 ± 0.8 × 107) (p < 0.0005). The latter yield, obtained from the iliac crest, was significantly higher than resulting from the BMAC harvested from the proximal tibia in the same group of patients (0.3 ± 0.2 × 107, p < 0.0005). This result was confirmed by the CFU-F analysis at day 10 (15.9 ± 19.4 vs 0.6 ± 1.0, p = 0.001) and day-20 (21.7 ± 23.0 vs 2.9 ± 4.2, p = 0.006). Conclusion Harvest site and age can affect the quality of BMAC. BMSCs obtained from iliac crest and proximal tibia present comparable mesenchymal markers expression as well as osteogenic and chondrogenic differentiation potential, but iliac crest BMAC presents a four times higher number of mononucleated cells with significantly higher clonogenic capacity compared to the tibia. BMAC of younger patients also had a three-time higher number of mononucleated cells. The identification of BMAC characteristics could help to optimize its preparation and to identify the most suitable indications for this orthobiologic treatment in the clinical practice.

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Section: Discussionmentioning

confidence: 99%

Bone marrow aspirate concentrate quality is affected by age and harvest site

Cavallo

Boffa

Merli

et al. 2022

Knee Surg Sports Traumatol Arthrosc

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“…There are various bone marrow needles available on the market namely (a) Jamshidi needle, (b) Modified Jamshidi needle, (c) Klima sternal needle, (d) Salah bone marrow aspiration needle, (e) Watherfield iliac crest bone marrow aspiration needle, and so on [ 84 ]. Feddahi et al, observed no significant difference regarding the quantity of MNCs and regenerative potency of MNCs in BMAC for either the Jamshidi needle (JAM) on one side and the Marrow Cellution ® Needle (AMC) on the other side when aspirated from the posterior iliac crest of 12 patients [ 101 ]. Oliver et al reported that a slightly higher quantity of MNCs was harvested by multiple-site (31 × 10 6 mL) than single-site (23 × 10 6 mL) bone marrow aspiration using the standard Jamshidi needle whereas a higher quantity of MSCs was retrieved using the single-site method (3486/mL concentrate) than the multiple-site method (2722/mL concentrate) [ 91 ].…”

Section: Variables In the Harvesting And Processing Technique Of Bone...mentioning

confidence: 99%

Impact of the Process Variables on the Yield of Mesenchymal Stromal Cells from Bone Marrow Aspirate Concentrate

et al. 2022

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Human bone marrow (BM) has been highlighted as a promising source of mesenchymal stromal cells (MSCs) containing various growth factors and cytokines that can be potentially utilized in regenerative procedures involving cartilage and bone. However, the proportion of MSCs in the nucleated cell population of BM is only around 0.001% to 0.01% thereby making the harvesting and processing technique crucial for obtaining optimal results upon its use in various regenerative processes. Although several studies in the literature have given encouraging results on the utility of BM aspiration concentrate (BMAC) in various regenerative procedures, there is a lack of consensus concerning the harvesting variables such as choice of anesthetic agent to be used, site of harvest, size of the syringe to be used, anticoagulant of choice, and processing variables such as centrifugation time, and speed. In this review article, we aim to discuss the variables in the harvesting and processing technique of BMAC and their impact on the yield of MSCs in the final concentrate obtained from them.

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“…Feddahi et al questions in an in vitro study if the needle design affects the regenerative potential of bone marrow aspirates, which are a promising alternative for the treatment of bone defects [ 5 ]. The authors harvested bone marrow from the posterior iliac crest of 12 patients either with Jamshidi needles or on the contralateral side with Marrow Cellution ® Needles.…”

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confidence: 99%