Does Nitrogen Fertilization Affect the Secondary Structures of Gliadin Proteins in Hypoallergenic Wheat?

Stawoska, Iwona; Waga, J; Wesełucha–Birczyńska, Aleksandra; Dziurka, Michał; Podolska, G.; Aleksandrowicz, Edyta; Skoczowski, Andrzej

doi:10.3390/molecules27175684

Cited by 8 publications

(4 citation statements)

References 75 publications

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“…The SS band, observed at a wavenumber of approximately 520 cm − 1 in the Raman spectra of patients diagnosed with infectious mononucleosis and consistent with Normal Mode Analysis, indicates the presence of the immunoglobulin gauche-gauche-trans (g-g-t) rotamer of in the variable (VL) domain [33,34]. This assignment is also consistent with the presence of the CS band at 675 cm − 1 .…”

Section: Results Of Standard Diagnostic Testssupporting

confidence: 78%

Raman spectroscopy of lymphocytes from patients with the Epstein-Barr virus infection

Pietruszewska,

Biesiada,

Czepiel

et al. 2023

Preprint

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In this study, Raman spectroscopy was applied to trace lymphocytes activation following contact with the Epstein-Barr virus (EBV) of the herpesvirus family. The biomarker of cell activation was found to be the 520 cm− 1 band, indicating formation of immunoglobulins. The blood samples were obtained from patients diagnosed with infectious mononucleosis and treated at the University Hospital in Kraków. The lymphocytes’ Raman spectra were collected using a mapping technique, exciting samples with a 514.5 nm line of Ar + laser. Measurements were performed on the 1st, 4th, 6th, 12th and 30th day of hospitalization, until the patient recovered. The highest intensity of the immunoglobulin marker was observed on the 4th day of hospitalization, while the results of the blood count of patients showed the greatest increase in the number of lymphocytes at the beginning of hospitalization. The blood of the healthy volunteers lacked activated lymphocytes. Some information was provided by the evaluation of B-cell activation by estimating the activated areas in the cells, which were determined by the presence of the Ig marker. The 900 cm− 1 band and band around 1450 cm− 1 were also analyzed as markers of the presence of the latent membrane protein, LMP2A (and 2B), of the EBV viral protein. The anomalous degree of depolarization observed in B-cells in the course of EBV infection appears to be due to the influence of a virus protein, disrupting BCR signal transduction.

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Section: Results Of Standard Diagnostic Testssupporting

confidence: 78%

Raman spectroscopy of lymphocytes from patients with the Epstein-Barr virus infection

Pietruszewska,

Biesiada,

Czepiel

et al. 2023

Preprint

Self Cite

View full text Add to dashboard Cite

show abstract

“…The SS band, observed at a wavenumber of approximately 520 cm −1 in the Raman spectra of patients diagnosed with infectious mononucleosis and consistent with Normal Mode Analysis, indicates the presence of the immunoglobulin gauche–gauche–trans (g–g–t) rotamer in the variable (VL) domain 29 , 30 . This assignment is also consistent with the presence of the CS band at 675 cm −1 .…”

Section: Resultssupporting

confidence: 68%

Raman spectroscopy of lymphocytes from patients with the Epstein–Barr virus infection

Pietruszewska,

Biesiada,

Czepiel

et al. 2024

Sci Rep

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In this study, Raman spectroscopy is applied to trace lymphocytes activation following contact with the Epstein–Barr virus (EBV) of the herpesvirus family. The biomarker of cell activation is found to be the 520 cm−1 band, indicating formation of immunoglobulins. The blood samples are obtained from patients diagnosed with infectious mononucleosis and treated at the University Hospital in Kraków. The lymphocytes’ Raman spectra are collected using a mapping technique, exciting samples with a 514.5 nm line of Ar + laser. Measurements are performed on the 1st, 4th, 6th, 12th and 30th day of hospitalization, until the patient has recovered. The highest intensity of the immunoglobulin marker is observed on the 4th day of hospitalization, while the results of the blood count of patients show the greatest increase in the number of lymphocytes at the beginning of hospitalization. No activated lymphocytes were observed in the blood of healthy volunteers. Some information is provided by the evaluation of B-cell activation by estimating the activated areas in the cells, which are determined by the presence of the Ig marker. The 900 cm−1 band and band around 1450 cm−1 are also analyzed as markers of the presence of the latent membrane protein, LMP2A (and 2B), of the EBV viral protein. The anomalous degree of depolarization observed in B-cells in the course of EBV infection appears to be due to the influence of a virus protein, disrupting BCR signal transduction.

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“…To determine the secondary structural content of Bet v1, the amide I band (spectral region of 1585-1710 cm -1 ) recorded for the liquid samples was decomposed using the PeakFit 4.12 (Systat Software, Inc., USA) program according to the previously presented procedure [45][46][47]. The analysis started with a baseline correction that used a linear function.…”

Section: Analysis Of the Amide I Spectral Regionmentioning

confidence: 99%

Air pollution in the places of Betula pendula growth and development changes the physicochemical properties and the main allergen content of its pollen

et al. 2023

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Pollen allergy becomes an increasing problem for humans, especially in the regions, where the air pollution level increases due to the traffic and urbanization. These factors may also affect the physiological activity of plants, causing changes in pollen allergenicity. The aim of the study was to estimate the influence of air pollutants on the chemical composition of birch pollen and the secondary structures of the Bet v1 protein. The research was conducted in seven locations in Malopolska region, South of Poland of a different pollution level. We have found slight fluctuations in the values of parameters describing the photosynthetic light reactions, similar spectra of leaf reflectance and the negligible differences in the discrimination values of the δ13C carbon isotope were found. The obtained results show a minor effect of a degree of pollution on the physiological condition B. pendula specimen. On the other hand, mean Bet v1 concentration measured in pollen samples collected in Kraków was significantly higher than in less polluted places (p = .03886), while FT-Raman spectra showed the most distinct variations in the wavenumbers characteristic of proteins. Pollen collected at sites of the increased NOx and PM concentration, show the highest percentage values of potential aggregated forms and antiparallel β-sheets in the expense of α-helix, presenting a substantial impact on chemical compounds of pollen, Bet v1 concentration and on formation of the secondary structure of proteins, what can influence their functions.

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Does Nitrogen Fertilization Affect the Secondary Structures of Gliadin Proteins in Hypoallergenic Wheat?

Cited by 8 publications

References 75 publications

Raman spectroscopy of lymphocytes from patients with the Epstein-Barr virus infection

Raman spectroscopy of lymphocytes from patients with the Epstein-Barr virus infection

Raman spectroscopy of lymphocytes from patients with the Epstein–Barr virus infection

Air pollution in the places of Betula pendula growth and development changes the physicochemical properties and the main allergen content of its pollen

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