Does plasma NGAL help for prediction of acute kidney injury in sepsis patients?: A systematic review and meta-analysis

Park, Hye Kyeong; Kim, Sollip; Lee, Sung-Soon; Kim, Hyun-Jung; Ahn, Hyeong Sik; Song, Ji Yang; Um, Tae Hyun; Cho, Chong-Rae; Jung, Hoon; Koo, Hyeon‐Kyoung; Sim, Yun Su; Song, Jae-Uk; Park, Joo-Hyun

doi:10.1183/13993003.congress-2015.pa2148

Cited by 4 publications

(7 citation statements)

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“…Additional analyses of AKI were performed on 48-hour samples from each group of the second CLP experiment. Neutrophil gelatinase-associated lipocalin-2 is a biomarker that increases rapidly in blood and urine following the onset of sterile AKI as well as SA-AKI (29,30). As shown in Figure 4A, plasma NGAL concentrations were markedly increased in CLP compared to sham control animals at 48 hours.…”

Section: Resultsmentioning

confidence: 97%

“…These latter analyses provided the clearest evidence for a modulatory effect of the double-dose hUC-MSC regimen on the severity of polymicrobial sepsis when compared to single-dose administration which, in contrast to results recently reported in a rat CLP model (24), was indistinguishable from the saline-treated CLP group across all indices examined. In particular, the clinically-relevant AKI biomarker, NGAL, proved to be a valuable discriminator of sepsis severity and treatment effect in this model and may be an important biomarker for future clinical trials of cell therapies in sepsis and SA-AKI (29,35).…”

Section: Discussionmentioning

confidence: 95%

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Comparison of Single and Repeated Dosing of Anti-Inflammatory Human Umbilical Cord Mesenchymal Stromal Cells in a Mouse Model of Polymicrobial Sepsis

Fazekas

Alagesan

Watson

et al. 2021

Preprint

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Mesenchymal stromal cells (MSCs) ameliorate pre-clinical sepsis and sepsis-associated acute kidney injury (SA-AKI) but clinical trials of single-dose MSCs have not indicated robust efficacy. This study investigated immunomodulatory effects of a novel MSC product (CD362-selected human umbilical cord-derived MSCs [hUC-MSCs]) in mouse endotoxemia and polymicrobial sepsis models.Initially, mice received intra-peritoneal (i.p.) lipopolysaccharide (LPS) followed by single i.p. doses of hUC-MSCs or vehicle. Next, mice underwent cecal ligation and puncture (CLP) followed by intravenous (i.v.) doses of hUC-MSCs at 4 hours or 4 and 28 hours. Analyses included serum/plasma assays of biochemical indices, inflammatory mediators and the AKI biomarker NGAL; multi-color flow cytometry of peritoneal macrophages (LPS) and intra-renal immune cell subpopulations (CLP) and histology/immunohistochemistry of kidney (CLP).At 72 hours post-LPS injections, hUC-MSCs reduced serum inflammatory mediators and peritoneal macrophage M1/M2 ratio. Repeated, but not single, hUC-MSC doses administered at 48 hours post-CLP resulted in increased survival, lower serum concentrations of inflammatory mediators, lower plasma NGAL and reversal of sepsis-associated depletion of intra-renal T-cell and myeloid cell subpopulations. Hierarchical clustering analysis of all 48-hour serum/plasma analytes demonstrated partial co-clustering of repeated-dose hUC-MSC CLP animals with a Sham group but did not reveal a distinct signature of response to therapy. It was concluded that repeated doses of CD362-selected hUC-MSCs are required to modulate systemic and local immune/inflammatory events in polymicrobial sepsis and SA-AKI. Inter-individual variability and lack of effect of single dose MSC administration in the CLP model are consistent with observations to date from early-phase clinical trials.

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Section: Resultsmentioning

confidence: 97%

Section: Discussionmentioning

confidence: 95%

Comparison of Single and Repeated Dosing of Anti-Inflammatory Human Umbilical Cord Mesenchymal Stromal Cells in a Mouse Model of Polymicrobial Sepsis

Fazekas

Alagesan

Watson

et al. 2021

Preprint

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show abstract

“…These latter analyses provided the clearest evidence for a modulatory effect of the double-dose hUC-MSC regimen on the severity of polymicrobial sepsis when compared to single-dose administration which, in contrast to results recently reported in a rat CLP model ( 24), was indistinguishable from the saline-treated CLP group across all indices examined. In particular, the clinically-relevant AKI biomarker, NGAL, proved to be a valuable discriminator of sepsis severity and treatment effect in this model and may be an important biomarker for future clinical trials of cell therapies in sepsis and SA-AKI (29,35).…”

Section: Discussionmentioning

confidence: 95%

“…Additional analyses of AKI were performed on 48-hour samples from each group of the second CLP experiment. Neutrophil gelatinaseassociated lipocalin-2 is a biomarker that increases rapidly in blood and urine following the onset of sterile AKI as well as SA-AKI (29,30). As shown in Fig.…”

Section: Repeated Dosing Of Huc-msc Modulated the Severity Of Acute Kidney Injury Following Clp-induced Polymicrobial Sepsis In Micementioning

confidence: 98%

Comparison of single and repeated dosing of anti-inflammatory human umbilical cord mesenchymal stromal cells in a mouse model of polymicrobial sepsis

Fazekas

Alagesan

Watson

et al. 2021

Preprint

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Background Mesenchymal stromal cells (MSCs) ameliorate pre-clinical sepsis and sepsis-associated acute kidney injury (SA-AKI) but clinical trials of single-dose MSCs have not indicated robust efficacy. This study investigated immunomodulatory effects of a novel MSC product (CD362-selected human umbilical cord-derived MSCs [hUC-MSCs]) in mouse endotoxemia and polymicrobial sepsis models. Methods Initially, mice received intra-peritoneal (i.p.) lipopolysaccharide (LPS) followed by single i.p. doses of hUC-MSCs or vehicle. Next, mice underwent cecal ligation and puncture (CLP) followed by intravenous (i.v.) doses of hUC-MSCs at 4 hours or 4 and 28 hours. Analyses included serum/plasma assays of biochemical indices, inflammatory mediators and the AKI biomarker NGAL; multi-color flow cytometry of peritoneal macrophages (LPS) and intra-renal immune cell subpopulations (CLP) and histology/immunohistochemistry of kidney (CLP). Results At 72 hours post-LPS injections, hUC-MSCs reduced serum inflammatory mediators and peritoneal macrophage M1/M2 ratio. Repeated, but not single, hUC-MSC doses administered at 48 hours post-CLP resulted in increased survival, lower serum concentrations of inflammatory mediators, lower plasma NGAL and reversal of sepsis-associated depletion of intra-renal T-cell and myeloid cell subpopulations. Hierarchical clustering analysis of all 48-hour serum/plasma analytes demonstrated partial co-clustering of repeated-dose hUC-MSC CLP animals with a Sham group but did not reveal a distinct signature of response to therapy. Conclusions It was concluded that repeated doses of CD362-selected hUC-MSCs are required to modulate systemic and local immune/inflammatory events in polymicrobial sepsis and SA-AKI. Inter-individual variability and lack of effect of single dose MSC administration in the CLP model are consistent with observations to date from early-phase clinical trials.

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“…Moreover, nanocomposite materials, in this case CNT-NPs (Carbon NanoTube-NanoParticles) nanocomposites achieve, due to synergy, improved properties, in particular electrical properties by integrating two types of materials: carbon and metal, therefore can be applied in sensors of chemical and biological substances, in which nanocomposites are a chemically active element [1,3,4]. The currently known nanocomposites consisting of carbon nanotubes together with nanoparticles of one (Au, Ag, Pt, Pd, Rh, Re) [5,6,[8][9][10] or more (Pd and Rh, Pt and Ru, Pt-Ru-Mo) [11][12][13] metals deposited on their surface are manufactured with diverse methods. In order to be able to apply CNT-NPs nanocomposites more effectively, a technology must be devised in modern industry enabling to control the uniform deposition of nanoparticles of a given metal onto the surface of carbon nanotubes.…”

Section: Introductionmentioning

confidence: 99%

The MWCNTs-Rh Nanocomposite Obtained By The New High-Temperature Method

Dobrzańska-Danikiewicz¹,

Cichocki²,

Łukowiec³

2015

Archives of Metallurgy and Materials

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A nanocomposite was fabricated during the research undertaken, consisting of multiwalled carbon nanotubes coated with rhodium nanoparticles by the new high-temperature method being the subject of the patent claim. High quality multiwalled carbon nanotubes (MWCNTs) with the length of 100÷500 nm and the diameter of 8÷20 nm obtained in advance with Catalytic Chemical Vapour Deposition (CVD) were employed in the investigations. The nanotubes manufactured under the own research contain small amounts of metallic impurities and amorphous carbon deposits. Multiwalled carbon nanotubes functionalisation in acids was applied to deposit rhodium nanoparticles onto the surface of carbon nanotubes, and then the material was placed in a solution being a precursor of rhodium nanoparticles. The material prepared was next placed in a quartz vessel and subjected to high-temperature reduction in the atmosphere of argon to deposit rhodium nanoparticles onto the surface of multiwalled carbon nanotubes. The following examinations were performed, respectively: MWCNTs fabrication, fabrication of a CNT-NPs (Carbon NanoTube-NanoParticles) nanocomposite material; the characterisation of the materials produced including examination of the structure and morphology, and the assessment of rhodium nanoparticles distribution on the surface of carbon nanotubes. Microand spectroscopy techniques were employed to characterise the structure of the nanocomposites obtained.Keywords: carbon nanotubes, nanocomposite materials, rhodium nanoparticles, transmission electron microscopy W ramach wykonanych badań wytworzono nanokompozyt składający się z wielościennych nanorurek węglowych pokrytych nanocząsteczkami rodu nową wysokotemperaturową metodą, będącą przedmiotem zgłoszenia patentowego. W badaniach wykorzystano wysokiej jakości wielościenne nanorurki węglowe MWCNTs o długości 100÷500 nm i średnicy 8÷20 nm uprzednio otrzymane w procesie katalityczno-chemicznego osadzania z fazy gazowej (CCVD). Wytworzone w ramach prac własnych nanorurki zawierają nieznaczne ilości zanieczyszczeń metalicznych i amorficznych depozytów węglowych. W celu osadzenia nanocząsteczek rodu na powierzchni nanorurek węglowych zastosowano funkcjonalizację wielościennych nanorurek węglowych w kwasach, następnie materiał umieszczono w roztworze będącym prekursorem nanocząsteczek rodu. Przygotowany materiał umieszczono w dalszej kolejności w kwarcowym naczyniu i poddano redukcji wysokotemperaturowej w atmosferze argonu w celu osadzenia nanocząsteczek rodu na powierzchni wielościennych nanorurek węglowych. Wykonane badania obejmują kolejno: wytworzenie MWCNTs, wytworzenie materiału nanokompozytowego typu nanorurki węglowe -nanocząsteczki (CNT-NPs), scharakteryzowanie wytworzonego materiału obejmujące badanie jego struktury i morfologii oraz ocenę rozmieszczenia nanocząsteczek rodu na powierzchni nanorurek węglowych. Dla scharakteryzowania struktury otrzymanych nanokompozytów zastosowano techniki mikro-i spektroskopowe.

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Does plasma NGAL help for prediction of acute kidney injury in sepsis patients?: A systematic review and meta-analysis

Cited by 4 publications

References 0 publications

Comparison of Single and Repeated Dosing of Anti-Inflammatory Human Umbilical Cord Mesenchymal Stromal Cells in a Mouse Model of Polymicrobial Sepsis

Comparison of Single and Repeated Dosing of Anti-Inflammatory Human Umbilical Cord Mesenchymal Stromal Cells in a Mouse Model of Polymicrobial Sepsis

Comparison of single and repeated dosing of anti-inflammatory human umbilical cord mesenchymal stromal cells in a mouse model of polymicrobial sepsis

The MWCNTs-Rh Nanocomposite Obtained By The New High-Temperature Method

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