Does sperm origin affect embryo morphokinetic parameters?

Abstract

Purpose The purpose of our study was to use time-lapse in order to evaluate the impact of sperm origin (fresh ejaculate or surgically retrieved) on embryo morphokinetic parameters and clinical outcome in intracytoplasmic sperm injection (ICSI) cycles. Methods This retrospective monocentric study was conducted in 485 unselected couples undergoing 604 ICSI cycles with embryo culture in the Embryoscope®. Among them, 445 couples underwent ICSI cycle with fresh ejaculated sperm and 40 with surgically retrieved sperm (26 with testicular sperm and 14 with epididymal sperm). Embryo morphokinetic parameters and clinical cycle outcome were compared between fresh ejaculated sperm and surgically retrieved sperm. A subgroup analysis was also conducted between testicular and epididymal sperm ICSI cycles.Results Clinical outcome was comparable between groups according to sperm origin. Although most early morphokinetic parameters were comparable between ejaculated and surgical sperm groups, a few parameters were significantly different between both groups, but with a considerable overlap in their distribution. Late cellular events occurred significantly later in the surgical sperm group than in the ejaculated sperm group. Conclusions Morphokinetic analysis did not allow us to identify clinically relevant differences between fresh ejaculate and surgically retrieved sperm groups. Further studies are needed, especially concerning the relationship between sperm origin and late morphokinetic parameters, such as blastocyst development.

Influence of human sperm origin, testicular or ejaculated, on embryo morphokinetic development

“…According our results, it is possible to say that sperm origin, and consequently its maturation stage, affects the timing of fertilisation and early and late cellular divisions during embryo development (Lammers et al, 2015). According our results, it is possible to say that sperm origin, and consequently its maturation stage, affects the timing of fertilisation and early and late cellular divisions during embryo development (Lammers et al, 2015).…”

“…In conclusion, the present study reveals a paternal effect in all stages of embryonic development following fertilisation. According our results, it is possible to say that sperm origin, and consequently its maturation stage, affects the timing of fertilisation and early and late cellular divisions during embryo development (Lammers et al, 2015).…”

“…A large number of morphokinetic parameters can be evaluated by TLM, such as timing of second polar body (IIPB) extrusion, appearance and fading of the pronuclei and cellular divisions. Until now, there are only few studies using TLM to evaluate embryo development in testicular patients (Lammers et al, 2015). A hierarchical model is based on a combination of morphological screening, the absence of exclusion criteria, timing and synchronisation of cellular divisions.…”

“…It is likely that a single model developed with TLM is not usable throughout all infertility centers and that local adjustments are needed according to different protocols and procedures used (Campbell et al, 2013;Kirkegaard, Hindkjaer, & Ingerslev, 2013;Minasi et al, 2016). Until now, there are only few studies using TLM to evaluate embryo development in testicular patients (Lammers et al, 2015).…”

High sperm DNA fragmentation delays human embryo kinetics when oocytes from young and healthy donors are microinjected

“…Previous research has assessed the relationship between embryo morphokinetics and embryo culture conditions (Basile et al, 2013) (Ciray et al, 2012), and a number of female factors such as age (Hampl & St ep an, 2013), ovarian reserve (Freour et al, 2012), body mass index (Bellver et al, 2013), type of ovarian stimulation (Muñoz et al, 2013), and tobacco consumption (Lammers et al, 2015) (Fr eour et al, 2013. However, very little is known about the influence of sperm parameters on embryo morphokinetics (Lammers et al, 2015).…”