Does supplemental 18:0 alleviate fish oil-induced milk fat depression in dairy ewes?

Toral, Pablo G.; Hervás, Gonzalo; Carreño, David; Frutos, Pilar

doi:10.3168/jds.2015-10304

Cited by 31 publications

(37 citation statements)

References 38 publications

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“…Diet supplementation with FO is an effective strategy to modulate ewe milk fatty acid composition towards a healthier profile810. However, the associated MFD constrains the dairy sheep industry due to the concomitant reduction of milk fat concentration.…”

Section: Discussionmentioning

confidence: 99%

“…In the nutrition field, different feeding strategies have been applied with the aim of increasing milk PUFA rates678910.…”

mentioning

confidence: 99%

“…Diet supplementation with marine lipids (namely, fish oil, FO) is an effective tool to modify the milk FA profile by increasing the content of certain bioactive lipids, such as n-3 PUFA and conjugated linoleic acid (CLA)7810. However, this type of supplementation has also been associated with milk fat depression (MFD) syndrome11.…”

mentioning

confidence: 99%

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Elucidating fish oil-induced milk fat depression in dairy sheep: Milk somatic cell transcriptome analysis

Suárez-Vega

Toral

Hervás

et al. 2017

Sci Rep

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In this study, RNA sequencing was used to obtain a comprehensive profile of the transcriptomic changes occurring in the mammary gland of lactating sheep suffering from fish oil-induced milk fat depression (FO-MFD). The milk somatic cell transcriptome analysis of four control and four FO-MFD ewes generated an average of 42 million paired-end reads per sample. In both conditions, less than 220 genes constitute approximately 89% of the total counts. These genes, which are considered as core genes, were mainly involved in cytoplasmic ribosomal proteins and electron transport chain pathways. In total, 117 genes were upregulated, and 96 genes were downregulated in FO-MFD samples. Functional analysis of the latter indicated a downregulation of genes involved in the SREBP signaling pathway (e.g., ACACA, ACSL, and ACSS) and Gene Ontology terms related to lipid metabolism and lipid biosynthetic processes. Integrated interpretation of upregulated genes indicated enrichment in genes encoding plasma membrane proteins and proteins regulating protein kinase activity. Overall, our results indicate that FO-MFD is associated with the downregulation of key genes involved in the mammary lipogenesis process. In addition, the results also suggest that this syndrome may be related to upregulation of other genes implicated in signal transduction and codification of transcription factors.

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Section: Discussionmentioning

confidence: 99%

“…In the nutrition field, different feeding strategies have been applied with the aim of increasing milk PUFA rates678910.…”

mentioning

confidence: 99%

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Elucidating fish oil-induced milk fat depression in dairy sheep: Milk somatic cell transcriptome analysis

Suárez-Vega

Toral

Hervás

et al. 2017

Sci Rep

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“…On this basis, an in vitro study was conducted to examine the changes in the BH of ALA in response to ruminal alterations associated with marine lipidinduced MFD in sheep. To meet this objective, an isotopic tracer approach was used in batch cultures of rumen microorganisms with inoculum collected from cannulated ewes fed either a diet without supplementation (control) or a diet supplemented with fish oil that was known to induce MFD (FO-MFD; Carreño et al, 2016;Toral et al, 2016).…”

Section: In Vitro Biohydrogenation Of 13 C-labeled α-Linolenic Acid Imentioning

confidence: 99%

“…However, the basis for the MFD observed in bovine and small ruminants supplemented with marine lipids has not been established yet (Harvatine et al, 2009;Carreño et al, 2016). In this regard, recent reports challenge the hypothesis of an involvement of the shortage of ruminal 18:0 and associated alterations in the maintenance of milk fat fluidity, and suggest the putative contribution of little known BH intermediates, such as trans-9,cis-11 CLA and trans-10,cis-15 18:2 (Kairenius et al, 2015;Toral et al, 2016;, which may derive from shifts in the ruminal metabolism of α-linolenic acid (cis-9,cis-12,cis-15 18:3, ALA; Lee and Jenkins, 2011;Alves and Bessa, 2014).…”

Section: Introductionmentioning

confidence: 99%

In vitro biohydrogenation of 13C-labeled α-linolenic acid in response to ruminal alterations associated with diet-induced milk fat depression in ewes

Toral

Hervás

Frutos

2019

Journal of Dairy Science

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The basis for marine lipid-induced milk fat depression (MFD) has not been established yet, but recent reports suggest the putative contribution of shifts in the ruminal metabolism of α-linolenic acid (ALA). To test this hypothesis, an isotopic tracer approach was used in batch cultures of rumen microorganisms with inoculum collected from cannulated ewes fed either a total mixed ration without lipid supplementation (control inoculum) or the same diet supplemented with 2% of fish oil, which is known to cause MFD in lactating sheep (FO-MFD inoculum). The [1-13 C]ALA was added at a dose of 1% of incubated dry matter and the proportions of 13 C-labeled fatty acids (FA) were examined after 24 h of incubation, using complementary gas chromatography and gas chromatography-combustion isotope ratio mass spectrometry (GC-C-IRMS) analyses. Expected differences in FA profiles were confirmed between control and FO-MFD inocula (e.g., large decreases in 18:0 and increases in most 18:1 and 18:2 intermediates, particularly trans isomers, to fish oil supply). The biohydrogenation of 13 ALA was extensive and yielded multiple metabolites, with a total of 48 chromatographic peaks showing 13 C enrichment, regardless of the inoculum type. However, although ALA was biohydrogenated through common pathways under standard or MFD conditions, large changes in the accumulation of 13 C-labeled FA suggest important differences in the relative contribution of each specific route. First, increased accumulation of trans-11-containing FA in FO-MFD incubations was accompanied by a general repression of the trans-13/14 pathway (supported by lower trans-13+14 18:1 or trans-11,trans-13 18:2 proportions), together with a lower production of cis FA (e.g., cis-9, -12, and -15 18:1 and some cis,cis 18:2). Results also downplayed the relevance of the trans-11 to trans-10 shift as an effective marker of diet-induced MFD in sheep, and challenged the involvement of some trans-10 intermediates (e.g., trans-10 18:1 and trans-10,cis-15 18:2) in the low-fat milk syndrome in this species. Conversely, increased abundance of most 18:3 intermediates (including some unidentified isomers) might be representative of ruminal alterations related to fish oil supplementation in ewes. Further research is necessary to examine the potential association between these findings and MFD in lactating animals.

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Milk Fat Globule in Ruminant: Major and Minor Compounds, Nutritional Regulation and Differences Among Species

Bernard

Bonnet

Delavaud

et al. 2018

Euro J Lipid Sci & Tech

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Recent knowledge is presented on the composition of ruminant milk fat fractions and the nutritional strategies known to alter their amount. The development of lipidomic and proteomic analyses has allowed for the characterization of minor components, such as proteins, liposoluble vitamins, and phospholipids of the milk fat globule (MFG), in addition to the triacylglycerols (TAG), which are the major constituents of the MFG core. Few differences in these components among ruminant species exist, and they have been outlined mainly on the fatty acids (FA) profile of the TAG, whereas comparative data are still lacking on vitamins and proteins. The effects of dietary treatments enriched in n‐3 polyunsaturated FA (PUFA) on the composition of the milk fat fraction are explored. In particular, pasture and plant oilseeds increase milk n‐3 PUFA and cis‐9,trans‐11 CLA and decrease saturated FA, whereas data with new feed resources, such as algae, are still rare. The peculiarities of the response of the milk fat to diets that induce a milk fat depression in cows but in lesser extent in small ruminants are described. The potential effects of polar lipids, proteins, and liposoluble vitamins of the MFG on human health are reviewed, highlighting the nutraceutical properties of milk. Practical Applications: This review provides an overview of the different components of the milk fat fraction in ruminant species and on nutritional strategies to alter their amounts to improve the nutritional quality of milk. Furthermore, this review presents recent data on species peculiarities of the milk fat fraction composition and of its response to nutritional factors, which offers a promising model to identify news levers of regulation of this fraction and foster the identification of new feeding strategies to better control milk fat composition and feed efficiency. This review synthesizes data on the composition of the milk fat fraction in ruminant species, reports advances in nutritional strategies to alter their amounts and species‐specific responses to nutrition, as well as the potential effects on human health of the major and minor components of this fraction.

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Does supplemental 18:0 alleviate fish oil-induced milk fat depression in dairy ewes?

Cited by 31 publications

References 38 publications

Elucidating fish oil-induced milk fat depression in dairy sheep: Milk somatic cell transcriptome analysis

Elucidating fish oil-induced milk fat depression in dairy sheep: Milk somatic cell transcriptome analysis

In vitro biohydrogenation of 13C-labeled α-linolenic acid in response to ruminal alterations associated with diet-induced milk fat depression in ewes

Milk Fat Globule in Ruminant: Major and Minor Compounds, Nutritional Regulation and Differences Among Species

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