Does the application of GaAlAs laser and platelet-rich plasma induce cell proliferation and increase alkaline phosphatase activity in human dental pulp stem cells?

Bidar, Maryam; Bahlakeh, Aminmohammad; Mahmoudi, Mahmoud; Ahrari, Farzaneh; Shahmohammadi, Reza; Jafarzadeh, Hamid

doi:10.1007/s10103-020-03239-0

Cited by 9 publications

(6 citation statements)

References 43 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…ALP assay was used to determine the osteogenic activity. Similar to the findings of prior investigations on DPSCs ( Nawam, 2019 ), the current analysis revealed that ALP activity increased following the administration of LLLT on day 14 ( Bidar et al, 2021 ). Cells affected by LLLT exhibited osteogenic differentiation capacity compared with those non-affected.…”

Section: Discussionsupporting

confidence: 89%

“…GaAlAs laser diode was the laser of choice in many types of research with variable wavelengths and energy intensities, applied to different lines of dental stem cells ( AlGhamdi et al, 2012 , İslam et al, 2021 ). It could induce hard tissue formation, increase ALP activity, dentinogenesis and formation of calcified nodules ( Nawam, 2019 , Bidar et al, 2021 ).…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Photo biomodulation of dental derived stem cells to ameliorate regenerative capacity: In vitro study

Medhat,

El-Zainy,

Fathy

2024

The Saudi Dental Journal

View full text Add to dashboard Cite

Section: Discussionsupporting

confidence: 89%

Section: Discussionmentioning

confidence: 99%

Photo biomodulation of dental derived stem cells to ameliorate regenerative capacity: In vitro study

Medhat,

El-Zainy,

Fathy

2024

The Saudi Dental Journal

View full text Add to dashboard Cite

“…The cellular identity was confirmed by short tandem repeat profiles. All cells were cultured in Dulbecco's modified Eagle's medium (DMEM) containing 10% fetal bovine serum (FBS) (Sigma), as formerly reported [25].…”

Section: Hcc-derived Cell Lines and Tissue Samplesmentioning

confidence: 99%

LPCAT1 overexpression promotes the progression of hepatocellular carcinoma

et al. 2021

Cancer Cell Int

View full text Add to dashboard Cite

Background Hepatocellular carcinoma (HCC) remains one of the most common malignant neoplasms. Lysophosphatidylcholine acyltransferase 1 (LPCAT1) plays a key role in the lipid remodelling and is correlated with various neoplasms. Nonetheless, the biological functions and molecular mechanisms of LPCAT1 underlying HCC remain obscure. Methods In the present study, we investigated the role of LPCAT1 in the progression of HCC. In-house RT-qPCR, tissue microarrays, and immunohistochemistry were performed to detect the expression levels and the clinical value of LPCAT1 in HCC. External datasets were downloaded to confirm the results. Proliferation, migration, invasiveness, cell cycle, and apoptosis assays were conducted to reveal the biological effects LPCAT1 has on SMMC-7721 and Huh7 cells. HCC differentially expressed genes and LPCAT1 co-expressed genes were identified to explore the molecular mechanisms underlying HCC progression. Results LPCAT1 showed upregulated expression in 3715 HCC specimens as opposed to 3105 non-tumour specimens. Additionally, LPCAT1 might be an independent prognostic factor for HCC. LPCAT1-knockout hampered cellular proliferation, migration, and metastasis in SMMC-7721 and Huh7 cells. More importantly, the cell cycle and chemical carcinogenesis were the two most enriched signalling pathways. Conclusions The present study demonstrated that increased LPCAT1 correlated with poor prognosis in HCC patients and fuelled HCC progression by promoting cellular growth, migration, and metastasis.

show abstract

“…In addition, the plasma component of PRP comprises albumin, fibrinogen, a number of immunoglobulins, and a number of bioactive compounds that play a crucial role in bone regeneration (40) . Moreover, Bida et al (41) showed that PRP has the capacity to boost alkaline phosphatase activity, a crucial sign of mineralization and bone growth. In addition, Wei et al (42) revealed that PRP unregulated the levels of osteogenesis-specific markers, such as runt-related transcription factor 2 (RUNX2), osterix (OSX), and osteopontin (OPN), which play crucial roles in the control of bone formation and mineralization.…”

Section: Discussionmentioning

confidence: 99%

Potential Effect of Platelet Rich Plasma as a Promoter of Bone Healing in Obese Rats (Histological and Ultrastructural Study)

Labah

Wahba

2023

Egyptian Dental Journal

View full text Add to dashboard Cite

The study aimed to examine the impact of platelet rich plasma (PRP) on the healing of a mandibular bone defect in obese rats. In this research, forty-eight adult male albino rats were divided into two equal groups; Group 1: were fed on standard diet. Group 2: were fed on high fat diet for four weeks for obesity inducing. A bone defect on the right side of mandible was created in each rat then all rats were subdivided into two equal subgroups. In subgroups 1.1 and 2.1, the bone defects were left without treating whereas in subgroups 1.2 and 2.2 the bone defects were filled with PRP. Two and four weeks postoperatively, six rats per group were euthanized and the samples were processed histological examination, X-ray elemental microanalysis and scanning electron microscopic examination. The results showed that rats received high fat diet and their bone defects left untreated revealed delayed bone healing and depicted significant decrease in bone calcium level in comparison with other subgroups. Interestingly, rats received high fat diet and their bone defects filled with platelet rich plasma revealed marked progress in bone healing and exhibited significant increase in bone calcium level in comparison with rats received high fat diet and with untreated bone defect. Conclusion: Obesity impaired the healing of mandibular bone defect. However, PRP has the ability to offset the negative effect of obesity and promote bone healing in obese rats. Therefore, it is highly recommended in obese individuals to fill the surgical bone defects with PRP.

show abstract

Does the application of GaAlAs laser and platelet-rich plasma induce cell proliferation and increase alkaline phosphatase activity in human dental pulp stem cells?

Cited by 9 publications

References 43 publications

Photo biomodulation of dental derived stem cells to ameliorate regenerative capacity: In vitro study

Photo biomodulation of dental derived stem cells to ameliorate regenerative capacity: In vitro study

LPCAT1 overexpression promotes the progression of hepatocellular carcinoma

Potential Effect of Platelet Rich Plasma as a Promoter of Bone Healing in Obese Rats (Histological and Ultrastructural Study)

Contact Info

Product

Resources

About