Does the cell number of 0PN embryos on day 3 affect pregnancy and neonatal outcomes following single blastocyst transfer?

Chen, Chen; Li, Wenzhi; Yin, Minzhi; Li, Menghui; Wu, Ling; Si, Jiqiang; Zhao, Leiwen; Li, Bin; Zheng, Yue; Lyu, Qifeng

doi:10.1186/s12884-022-04492-7

Cited by 7 publications

(3 citation statements)

References 53 publications

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“…In this study, D5 good blastocyst formation rate was higher in 0PN embryos and most of the transferred blastocysts ( 80%) were D5 blastocysts and good-quality blastocysts, which may be the reason for no signi cant differences in CPR and LBR between the 0PN and 2PN blastocyst transfers before and after matching. And recent studies also supported our results [9,34,42,43]. For neonatal outcomes, 0PN-VBT was associated with increased birthweight and a higher risk for very LGA newborns was reported by Li et al, [42], which was not observed in the study.…”

Section: Discussionsupporting

confidence: 90%

Developmental competence and neonatal outcomes of nonpronuclear zygotes following single vitrified-warmed blastocyst transfers using propensity score matching analysis

Zhu,

Wang,

Cao

et al. 2023

Arch Gynecol Obstet

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Purpose To investigate developmental competence and neonatal outcomes of nonpronuclear (0PN) zygotes following single vitri ed-warmed blastocyst transfers (VBT).Methods The clinical, laboratorial and neonatal data of 996 patients with ≤38 years who underwent blastocyst culture and single VBT were retrospectively analyzed. The pregnancy and neonatal outcomes of VBT were compared between 0PN and 2PN blastocysts using propensity score matching (PSM). Moreover, Day 3 (D3) embryo development and blastocyst formation were compared between 0PN and 2PN zygotes.ResultsThere were no signi cant differences in clinical pregnancy rate (CPR), live birth rate (LBR) and neonatal outcomes of VBT between the 0PN and 2PN blastocysts irrespectively of whether PSM was used. However, early abortion rate (EAR) was higher in blastocysts from 0PN D3 embryos 10 cells (p 0.05) before PSM. Moreover, the early developmental competence of 0PN zygotes was different from that of 2PN zygotes presenting higher percentages of D3 embryos ≤6 cells (p 0.01) and 10 cells (p 0.01), lower available blastocyst formation rate (ABFR) (p 0.01) and good-quality blastocyst formation rate (GBFR) (p 0.01) in D3 embryos with 4-6 cells. ABFR and GBFR increased with cell number when compared among embryos with 4-6 cells, 7-10 cells and 10 cells, irrespectively of 0PN or 2PN embryos.ConclusionThe early developmental competence of 0PN zygotes was different from that of 2PN zygotes, but did not in uence pregnancy and neonatal outcomes following VBT. ABFR and GBFR increased with cell number, irrespectively of 0PN or 2PN embryos.What does this study add to the clinical work?The early developmental competence of 0PN zygotes was different from that of 2PN zygotes, but did not in uence pregnancy and neonatal outcomes following VBT.The rates of available blastocyst and good-quality blastocyst increased with cell number of D3 embryos, irrespectively of 0PN or 2PN embryos.

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Section: Discussionsupporting

confidence: 90%

Developmental competence and neonatal outcomes of nonpronuclear zygotes following single vitrified-warmed blastocyst transfers using propensity score matching analysis

Zhu,

Wang,

Cao

et al. 2023

Arch Gynecol Obstet

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“…Blastocyst culture is commonly recommended to select development potential for 0PN embryos [22]. Chen et al reported that the available blastocyst rates of 0PN embryos were decreased when compared with those of 2PN embryos, and the cell number of 0PN embryos on day 3 affected subsequent blastocyst formation [23]. The available blastocyst rate of 0PN embryos was higher for the ≥6 cells than <6 cells day 3 embryos.…”

Section: Discussionmentioning

confidence: 99%

Clinical outcomes of frozen-thawed blastocysts from zygotes with no or one pronucleus for in vitro fertilization and intracytoplasmic sperm injection cycles

Jin¹,

Xue²,

Liu³

et al. 2023

Preprint

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Purpose To investigate the clinical outcomes of in vitro fertilization/intracytoplasmic sperm injection (IVF/ICSI) cycles using frozen-thawed blastocyst transfers derived from zygotes with no (0PN) or one pronucleus (1PN). Methods This retrospective study included 7084 0PN, 2238 1PN and 72266 two-pronuclear (2PN) embryos cultured into blastocyst stage from 19631 IVF and 12377 ICSI cycles between March 2018 and December 2021. Developmental potential and clinical outcomes of 0PN, 1PN and 2PN embryos were analyzed. A total of 290 0PN-derived, 92 1PN-derived and 1906 2PN-derived single frozen-thawed blastocyst transfers were performed. Chromosome analysis of 0PN-, 1PN- or 2PN-derived blastocysts used Infinium Asian Screening Array gene chip or next-generation sequencing. Results The available blastocyst rates of 0PN and 1PN embryos were significantly lower than those of 2PN embryos in both IVF and ICSI cycles. Single 0PN and 1PN blastocysts transferred in frozen-thawed cycles resulted in a similar clinical pregnancy rate, live birth rate and miscarriage rate compared with 2PN blastocysts, in both IVF and ICSI cycles. Genetic analysis showed that euploid rates of 0PN- and 1PN-derived blastocysts used for ICSI cycles were similar to that of 2PN-derived blastocysts. Conclusion Our study indicated that 0PN- and 1PN-derived blastocysts resulted in a similar CPR and LBR as 2PN-derived blastocysts. The 0PN- and 1PN-derived blastocysts from ICSI cycles can be transferred as well as those from IVF cycles when the number of 2PN-derived blastocysts is insufficient.

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“…Chen et al reported a decrease in the available blastocyst rates of 0PN embryos compared with those of 2PN embryos, and the cell number of 0PN embryos on day 3 affected subsequent blastocyst formation [ 21 ]. The available blastocyst rate of 0PN embryos was higher for ≥ 6-cell than < 6-cell day 3 embryos.…”

Section: Discussionmentioning

confidence: 99%

Clinical outcomes of frozen–thawed blastocysts from zygotes with no or one pronucleus for in vitro fertilization and intracytoplasmic sperm injection cycles

Jin

Xue

Liu

et al. 2023

Arch Gynecol Obstet

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Purpose To investigate the clinical outcomes of in vitro fertilization (IVF) and intracytoplasmic sperm injection (ICSI) cycles using frozen–thawed blastocyst transfers derived from zygotes with no (0PN) or one pronucleus (1PN). Methods This retrospective study included 7084 0PN, 2238 1PN, and 72,266 two pronuclear (2PN) embryos cultured to the blastocyst stage from 19,631 IVF and 12,377 ICSI cycles between March 2018 and December 2021. Developmental potential and clinical outcomes of 0PN, 1PN, and 2PN embryos were analyzed. A total of 290 0PN-, 92 1PN-, and 1906 2PN-derived single frozen–thawed blastocyst transfers were performed. Chromosome euploid rates of 0PN-, 1PN-, and 2PN-derived blastocysts were analyzed by next-generation sequencing. Euploid 0PN- and 1PN-derived blastocysts underwent subsequent Infinium Asian Screening Array gene chip analysis to detect ploidy alterations. Results Available blastocyst rates of 0PN and 1PN embryos were significantly lower than those of 2PN embryos in both IVF and ICSI cycles. Single 0PN and 1PN blastocysts transferred in frozen–thawed cycles resulted in a similar clinical pregnancy rate, miscarriage rate, live birth rate, and neonatal outcome to 2PN blastocysts in IVF and ICSI cycles. Genetic analysis showed that euploid rates of 0PN- and 1PN-derived blastocysts used for ICSI cycles were similar to that of 2PN-derived blastocysts. Conclusion Our study indicated that 0PN- and 1PN-derived blastocysts resulted in similar clinical outcomes to 2PN-derived blastocysts. The 0PN- and 1PN-derived blastocysts from ICSI cycles can be transferred as well as those from IVF cycles when the number of 2PN-derived blastocysts is insufficient.

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Does the cell number of 0PN embryos on day 3 affect pregnancy and neonatal outcomes following single blastocyst transfer?

Cited by 7 publications

References 53 publications

Developmental competence and neonatal outcomes of nonpronuclear zygotes following single vitrified-warmed blastocyst transfers using propensity score matching analysis

Developmental competence and neonatal outcomes of nonpronuclear zygotes following single vitrified-warmed blastocyst transfers using propensity score matching analysis

Clinical outcomes of frozen-thawed blastocysts from zygotes with no or one pronucleus for in vitro fertilization and intracytoplasmic sperm injection cycles

Clinical outcomes of frozen–thawed blastocysts from zygotes with no or one pronucleus for in vitro fertilization and intracytoplasmic sperm injection cycles

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