Does the intestinal bifidobacterial colonisation affect bacterial translocation?

Romond, Marie-Bénédicte; Colavizza, Michel; Mullié, Catherine; Kalach, Nicolas; Kremp, O.; Mielcarek, Christine; Izard, D.

doi:10.1016/j.anaerobe.2007.09.003

Cited by 34 publications

(28 citation statements)

References 31 publications

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“…However, an increase of a single species of bacteria, B. dorei, in cases before seroconversion was observed in another study (8). Bacteria in the Bacteroides genus, particularly the B. fragilis group, which includes B. fragilis, B. ovatus, and B. vulgatus, were found to enhance bacterial translocation (54). Bacteroides lipopolysaccharide (LPS) is structurally distinct from Escherichia coli LPS and inhibits innate immune signaling and endotoxin tolerance, suggesting that it may preclude immune development in early infancy (9).…”

Section: Discussionmentioning

confidence: 99%

Intestinal virome changes precede autoimmunity in type I diabetes-susceptible children

Zhao

Vatanen

Droit

et al. 2017

Proc. Natl. Acad. Sci. U.S.A.

254

240

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Viruses have long been considered potential triggers of autoimmune diseases. Here we defined the intestinal virome from birth to the development of autoimmunity in children at risk for type 1 diabetes (T1D). A total of 220 virus-enriched preparations from serially collected fecal samples from 11 children (cases) who developed serum autoantibodies associated with T1D (of whom five developed clinical T1D) were compared with samples from controls. Intestinal viromes of case subjects were less diverse than those of controls. Among eukaryotic viruses, we identified significant enrichment ofCircoviridae-related sequences in samples from controls in comparison with cases. Enterovirus, kobuvirus, parechovirus, parvovirus, and rotavirus sequences were frequently detected but were not associated with autoimmunity. For bacteriophages, we found higher Shannon diversity and richness in controls compared with cases and observed that changes in the intestinal virome over time differed between cases and controls. Using Random Forests analysis, we identified disease-associated viral bacteriophage contigs after subtraction of age-associated contigs. These disease-associated contigs were statistically linked to specific components of the bacterial microbiome. Thus, changes in the intestinal virome preceded autoimmunity in this cohort. Specific components of the virome were both directly and inversely associated with the development of human autoimmune disease.

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Section: Discussionmentioning

confidence: 99%

Intestinal virome changes precede autoimmunity in type I diabetes-susceptible children

Zhao

Vatanen

Droit

et al. 2017

Proc. Natl. Acad. Sci. U.S.A.

254

240

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“…Such studies have highlighted an association between Bacteroides and enhanced intestinal bacterial translocation, resulting in b-cell autoimmunity, associated with T1D (de Goffau et al, 2013). Bifidobacteria have previously been associated with an inhibition of bacterial translocation (Duffy, 2000;Romond et al, 2008;Wang et al, 2004) and it has recently been hypothesized (de Goffau et al, 2013) that bifidobacteria may inhibit the translocation and growth of Bacteroides as they compete for space and/or adherence (Stecher & Hardt, 2008) and nutrients (Gibson et al, 1996). Furthermore, bifidobacteria enhance the intestinal epithelial barrier function (Liévin et al, 2000) by increasing the thickness of the mucous layer (Kleessen & Blaut, 2005;Kleessen et al, 2003).…”

Section: Discussionmentioning

confidence: 99%

Streptozotocin-induced type-1-diabetes disease onset in Sprague–Dawley rats is associated with an altered intestinal microbiota composition and decreased diversity

et al. 2015

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There is a growing appreciation that microbiota composition can significantly affect host health and play a role in disease onset and progression. This study assessed the impact of streptozotocin (STZ)-induced type-1-diabetes (T1D) on intestinal microbiota composition and diversity in Sprague-Dawley rats, compared with healthy controls over time. T1D was induced by injection of a single dose (60 mg STZ kg "1 ) of STZ, administered via the intraperitoneal cavity. Total DNA was isolated from faecal pellets at weeks 0 (pre-STZ injection), 1, 2 and 4 and from caecal content at week 5 from both healthy and T1D groups. High-throughput 16S rRNA sequencing was employed to investigate intestinal microbiota composition. The data revealed that although intestinal microbiota composition between the groups was similar at week 0, a dramatic impact of T1D development on the microbiota was apparent post-STZ injection and for up to 5 weeks. Most notably, T1D onset was associated with a shift in the Bacteroidetes : Firmicutes ratio (P,0.05), while at the genus level, increased proportions of lactic acid producing bacteria such as Lactobacillus and Bifidobacterium were associated with the later stages of T1D progression (P,0.05). Coincidently, T1D increased caecal lactate levels (P,0.05). Microbial diversity was also reduced following T1D (P,0.05). Principle co-ordinate analyses demonstrated temporal clustering in T1D and control groups with distinct separation between groups. The results provide a comprehensive account of how T1D is associated with an altered intestinal microbiota composition and reduced microbial diversity over time.

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“…[7,12] It is well known that the intestinal microbiota play an important role in human health. [13 -16] For example, a normal, healthy balance of beneficial bacteria in the human intestine shapes the host nutrient environment, [17] regulates fat storage, [18] inhibits bacterial translocation protecting the gut against potential pathogenic bacterium, [19,20] and is essential to establishing a healthy mucosal immune system. [21] However, gut microflora can also act as a causative factor in human disease [22,23] with Mycobacterium, Campylobacter, and Clostridium species thought to be factors in a number of inflammatory bowel diseases (IBDs).…”

Section: Introductionmentioning

confidence: 99%

NMR‐based metabonomics analysis of mouse urine and fecal extracts following oral treatment with the broad‐spectrum antibiotic enrofloxacin (Baytril)

Romick-Rosendale

Goodpaster

Hanwright

et al. 2009

Magnetic Reson in Chemistry

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The human gastrointestinal tract is home to hundreds of species of bacteria and the balance between beneficial and pathogenic bacteria plays a critical role in human health and disease. The human infant, however, is born with a sterile gut and the complex gastrointestinal host/bacterial ecosystem is only established after birth by rapid bacterial colonization. Composition of newborn gut flora depends on several factors including type of birth (Ceasarian or natural), manner of early feeding (breast milk or formula), and exposure to local, physical environment. Imbalance in normal, healthy gut flora contributes to several adult human diseases including inflammatory bowel (ulcerative colitis and Crohn's disease) and Clostridium difficile associated disease, and early childhood diseases such as necrotizing enterocolitis. As a first step towards characterization of the role of gut bacteria in human health and disease, we conducted an 850 MHz (1)H nuclear magnetic resonance spectroscopy study to monitor changes in metabolic profiles of urine and fecal extracts of 15 mice following gut sterilization by the broad-spectrum antibiotic enrofloxacin (also known as Baytril). Ten metabolites changed in urine following enrofloxacin treatment including decreased acetate due to loss of microbial catabolism of sugars and polysaccharides, decreased trimethylamine-N-oxide due to loss of microbial catabolism of choline, and increased creatine and creatinine due to loss of microbial enzyme degradation. Eight metabolites changed in fecal extracts of mice treated with enrofloxacin including depletion of amino acids produced by microbial proteases, reduction in metabolites generated by lactate-utilizing bacteria, and increased urea caused by loss of microbial ureases.

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Does the intestinal bifidobacterial colonisation affect bacterial translocation?

Cited by 34 publications

References 31 publications

Intestinal virome changes precede autoimmunity in type I diabetes-susceptible children

Intestinal virome changes precede autoimmunity in type I diabetes-susceptible children

Streptozotocin-induced type-1-diabetes disease onset in Sprague–Dawley rats is associated with an altered intestinal microbiota composition and decreased diversity

NMR‐based metabonomics analysis of mouse urine and fecal extracts following oral treatment with the broad‐spectrum antibiotic enrofloxacin (Baytril)

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