Does the Nucleoid Determine Cell Dimensions in Escherichia coli?

Zaritsky, Arieh; Vollmer, Waldemar; Männik, Jaan; Liu, Chenli

doi:10.3389/fmicb.2019.01717

Cited by 10 publications

(7 citation statements)

References 58 publications

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“…Significant drops in both cell length and nucleoid area, associated with increased cell diameter were also observed here ( Fig. 3), suggesting that enhanced cell division and remodelling of cell dimensions are general, perhaps universal, immediate reactions to arresting DNA replication in bacteria [6].…”

Section: Results Discussion and Conclusionsupporting

confidence: 66%

Transient enhanced cell division by blocking DNA synthesis in Escherichia coli

et al. 2020

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Duplication of the bacterial nucleoid is necessary for cell division hence specific arrest of DNA replication inhibits divisions culminating in filamentation, nucleoid dispersion and appearance of a-nucleated cells. It is demonstrated here that during the first 10 min however, Escherichia coli enhanced residual divisions: the proportion of constricted cells doubled (to 40%), nucleoids contracted and cells remodelled dimensions: length decreased and width increased. The preliminary data provides further support to the existence of temporal and spatial couplings between the nucleoid/replisome and the sacculus/divisome, and is consistent with the idea that bacillary bacteria modulate width during the division process exclusively.

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Section: Results Discussion and Conclusionsupporting

confidence: 66%

Transient enhanced cell division by blocking DNA synthesis in Escherichia coli

et al. 2020

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“…This change occurs firstly by increasing W up to a limit, then by branching 23 . The W-limit is consistent with the concept of existing eclipse 24 : a minimal distance from oriC needed for a replisome to reach before the next replisome can move forward to replicate the chromosome away from the new, temporarily abandoned initiating oriC (and see 25,26 ). The recent finding that the rate of in vivo replication is not constant along the chromosome but rather oscillates, and in a still enigmatic manner 20 , complicates matters further, more so in thymine-limited slow replication rates as needed for accurate extensions of the analyses such as performed here.…”

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confidence: 71%

Cell Width inEscherichia coli: Interrogating Coupling to Nucleoid Structure

Howard

Rabinovitch

Yehezkel

et al. 2023

Preprint

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Cell dimensions of rod-shaped bacteria such as Escherichia coli are connected to mass growth and chromosome replication. It divides 20 min after termination of the replication cycle that initiates 40 min earlier at a relatively constant mass. Cells enlarge by elongation only, but at faster growth in richer media they are also wider. Width determination occurs in the divisome during the division process, coupled, temporally and spatially, to the ratio between the rates of growth and replication. The elusive signal directing the mechanism for width determination is related to the tightly linked duplications of the nucleoid (DNA) and the sacculus (peptidoglycan), the only two structures (macro-molecules) existing in a single copy. Six biologically meaningful parameters related to the key number of replication positions are reasonable candidates to convey such a signal. The current analysis discovered that of these, nucleoid complexity is the most likely parameter affecting cell width. As a corollary, a new, indirect approach to estimate replication rate is revealed.

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“…[12][13][14][15][16][17][18] But then it has to be aided by some other hitherto unknown processes driven by proteins, as entropic repulsion is too weak 8 to have segregation within a time scale of 60 minutes, as seen in vivo. [19][20][21] This has spawned a series of experiments over the last two decades.…”

Section: Introductionmentioning

confidence: 99%