Does the previous exposure to copper alter the pattern of avoidance by zebrafish in a copper gradient scenario? Hypothesis of time-delayed avoidance due to pre-acclimation

Araújo, Cristiano V.M.; Pontes, João Rodolfo S.; Blasco, Julián

doi:10.1016/j.scitotenv.2019.133703

Cited by 10 publications

(4 citation statements)

References 47 publications

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“…By separating three compartments by narrow links, we could contain the diffusion of nicotine and generate one nicotine compartment on one side and a compartment without nicotine on the other. The gradient design has been applied successfully in a copper avoidance test for zebrafish ( Araújo et al, 2019 ). Based on food dye diffusion experiments some nicotine it likely to diffuse into the center compartment but to a much lesser degree into the water compartment connected to the center compartment.…”

Section: Discussionmentioning

confidence: 99%

Identification of nicotine-seeking and avoiding larval zebrafish using a new three-choice behavioral assay

Schneider

Pearson

Harris

et al. 2023

Front. Mol. Neurosci.

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IntroductionNicotine dependence is one of the main causes of preventable diseases in the United States. Nicotine-seeking and avoidance behavioral assays in larval zebrafish could be used for identifying potential new pharmacotherapeutics in an early phase of drug discovery and could facilitate the identification of genes and genomic variations associated with nicotine-seeking and avoidance behavior.MethodsA new three-choice behavioral assay has been developed for the identification of nicotine-seeking and avoiding larval zebrafish. The three choices are represented by three compartments of a gradient maze. Video-recording and subsequent quantitative analysis of the swimming track was carried out using EthovisionXT (Noldus).ResultsThree behavioral phenotypes could be identified. Nicotine-seeking larval zebrafish occupied nicotine compartments for longer periods and entered the nicotine-containing compartments most frequently. Nicotine-avoiders spent most of the cumulative time in the water compartment or entered the water compartment most frequently. Non-seekers remained in the center compartment for most of the time. In the gradient maze, about 20–30% of larval zebrafish had a preference for low nicotine concentrations whereas nicotine avoidance was stronger at higher nicotine concentrations. Lower concentrations of nicotine (0.63 μM, 6.3 μM) resulted in higher percentages of nicotine seekers whereas high nicotine concentrations (63 μM, 630 µM) resulted in higher percentages of nicotine avoiders. Pre-treatment of larval zebrafish with nicotine slightly increased the percentage of nicotine avoiders at lower nicotine concentrations. Treatment with varenicline strongly increased the percentage of nicotine avoiders at lower nicotine concentrations.ConclusionThe results show that larval zebrafish have individual preferences for nicotine that could change with drug treatment. The three-choice gradient maze assay for larval zebrafish provides a new testing paradigm for studying the molecular and cellular mechanisms of nicotine action and the discovery of potential new pharmacotherapeutics for the treatment of smoking cessation.

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Section: Discussionmentioning

confidence: 99%

Identification of nicotine-seeking and avoiding larval zebrafish using a new three-choice behavioral assay

Schneider

Pearson

Harris

et al. 2023

Front. Mol. Neurosci.

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“…Two environmentally relevant stressors were used: copper, as a stressor related to contamination, and fish kairomones, as a stressor simulating the presence of predators. To simulate the contamination scenarios, two copper concentrations were used: 0.5 μg/L, representing the environmental levels found in some estuaries and deltas of southwest Spain (González-Ortegón et al, 2019), and 25 μg/L, representing a high contamination level capable of triggering an avoidance response in cladocerans (Lopes et al, 2004;Gutierrez et al, 2012), fish (Araújo et al, 2019b;Islam et al, 2019), and shrimps (Vera-Vera et al, 2019;Araújo et al, 2020b). Both concentrations were prepared from a standard solution (Merck; 1000 mg/L) by dilution with saline water to the two concentrations selected.…”

Section: Stressors and Attractive Stimulusmentioning

confidence: 99%

Experimental Evidence of Contamination Driven Shrimp Population Dynamics: Susceptibility of Populations to Spatial Isolation

Guerrero¹,

Rodríguez-Ruiz²,

Castro³

et al. 2021

SSRN Journal

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“…Liver tissue [27] and increases the development of bone and muscle diseases [28]. As for copper ion pollution, it has toxic effects on the environment and sh farming [29].…”

Section: Introductionmentioning

confidence: 99%

Comparative Analysis of Hydrogel Adsorption/Desorption with and without Surfactants

Dhahir,

Braihi,

Habeeb

2024

Preprint

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In this study, the hydrogel (SAP-1) was prepared by grafting acrylic acid-co-acrylamide onto pullulan (Pul-g-Poly (acrylic acid-co-acrylamide)) and the sponge hydrogel (SAP-2) was prepared by adding the surfactant sodium dodecyl benzene. sulfonate (SDBS) by free radical solution polymerization. Several properties were used to determine the efficiency of the prepared hydrogels such as Fourier transform infrared spectroscopy, hydrogen nuclear magnetic resonance (1H NMR), atomic absorption spectroscopy, and field emission scanning electron microscopy (FE-SEM) to further understand the composition and properties of the hydrogels. On the other hand, the absorption kinetics were studied and analyzed with the experimental equilibrium capacities of the prepared hydrogels were determined. The results demonstrated that each of the prepared hydrogels is an effective adsorbent of cadmium (II), copper (II), and nickel (II) ions. SAP-2 gel is characterized by its high ability to absorb cadmium (II) ions at a rate of 190.72 mg/g, followed by SAP-1 gel for absorbing cadmium (II) ions at a rate of 146.9 mg/g and copper (II) ions at a rate of 154 mg/g. The hydrogel (SAP- 2) with its high ability to repeat the adsorption-absorption cycles 3 times for cadmium (II) ions, the absorption capacities were 190.72 mg/g, 100.43 mg/g, and 19.64 mg/g at first, second, and third cycles, respectively. Based on the above results, all the prepared hydrogels are good candidates for adsorption and desorption of cadmium (II), copper (II), and nickel (II) ions.

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Does the previous exposure to copper alter the pattern of avoidance by zebrafish in a copper gradient scenario? Hypothesis of time-delayed avoidance due to pre-acclimation

Cited by 10 publications

References 47 publications

Identification of nicotine-seeking and avoiding larval zebrafish using a new three-choice behavioral assay

Identification of nicotine-seeking and avoiding larval zebrafish using a new three-choice behavioral assay

Experimental Evidence of Contamination Driven Shrimp Population Dynamics: Susceptibility of Populations to Spatial Isolation

Comparative Analysis of Hydrogel Adsorption/Desorption with and without Surfactants

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