Objective: To explore whether initiatives to promote fruit and vegetables in primary schools are associated with changes in children's diet. Design: Cross-sectional dietary survey. Main outcome measures were intakes of fruit, vegetables and key nutrients; and a score for initiatives promoting fruit and vegetables in school. Setting: One hundred and twenty-nine English primary schools. Subjects: Year 2 children (aged 6-7 years, n 2530). Results: In schools running a gardening club, children ate more vegetables, 120 (95 % CI 111, 129) g/d, compared with those that did not, 99?3 (95 % CI 89?9, 109) g/d; and where parents were actively involved in school initiatives to promote fruit and vegetables, children's intake of vegetables was higher, 117 (95 % CI 107, 128) g/d, compared with those where parents were not involved, 105 (95 % CI 96?2, 114) g/d. In schools that achieved a high total score (derived from five key types of initiatives to promote fruit and vegetables in school) children ate more vegetables, 123 (95 % CI 114, 132) g/d, compared with those that did not, 97?7 (95 % CI 88?7, 107) g/d. Conclusions: Gardening, parental involvement and other activities promoting fruit and vegetables to children in school may be associated with increased intake of vegetables but not fruit. These effects were independent of deprivation status and ethnicity.