Does translational ambiguity increase during cell differentiation?

Picard-Bennoun, Marguerite

doi:10.1016/0014-5793(82)81094-6

Cited by 27 publications

(10 citation statements)

References 29 publications

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“…This suggests a further explanation for the important role of SpoVC in sporulation. Genetic studies in several lower eukaryotes strongly suggest that an increase in translational ambiguity may be a requirement at critical stages of cell differentiation (Picard‐Bennoun, 1982; Silar and Picard, 1994). Increased ambiguity is very likely to be accompanied by increased peptidyl‐tRNA drop‐off and an increased requirement for SpoVC.…”

Section: Discussionmentioning

confidence: 99%

Peptidyl‐tRNA hydrolase in Bacillus subtilis, encoded by spoVC, is essential to vegetative growth, whereas the homologous enzyme in Saccharomyces cerevisiae is dispensable

Menez

Buckingham²,

Zamaroczy

et al. 2002

Molecular Microbiology

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Summary Peptidyl‐tRNA hydrolase in Escherichia coli, encoded by pth, is essential for recycling tRNA molecules sequestered as peptidyl‐tRNA as a result of pre‐mature dissociation from the ribosome during translation. Genes homologous to pth are present in other bacteria, yeast and man, but not in archaea. The homologous gene in Bacillus subtilis, spoVC, was first identified as a gene involved in sporulation. A second copy of spoVC, under the control of the xyl promoter, was integrated into B. subtilis at the amy locus. In this background, interruption of the original gene was possible provided that expression of the copy at the amy locus was induced. When spoVC was interrupted, both vegetative growth and sporulation were dependent on xylose, showing that SpoVC is essential. The role of SpoVC in sporulation is discussed and appears to be consistent with previous hypotheses that a relaxation of translational accuracy may occur during sporulation. The homologous gene in Saccharomyces cerevisiae, yHR189W, has been interrupted in both haploid and diploid strains. The mutant haploid strains remain viable, as do the yHR189W mutant spores obtained by tetrad dis‐section, with either glucose or glycerol as carbon source, showing that the yHR189W gene product is dispensable for cell growth and for mitochondrial respiration.

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Section: Discussionmentioning

confidence: 99%

Peptidyl‐tRNA hydrolase in Bacillus subtilis, encoded by spoVC, is essential to vegetative growth, whereas the homologous enzyme in Saccharomyces cerevisiae is dispensable

Menez

Buckingham²,

Zamaroczy

et al. 2002

Molecular Microbiology

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“…The fact that a high translational fidelity blocks sporulation in Podospora is discussed elsewhere (Dequard-Chablat & Coopin-Raynal, 1984) within the context of a more general hypothesis proposed by Picard-Bennoun (1982).…”

Section: Discussionmentioning

confidence: 96%

Ribosomal suppressors inPodospora anserina: evidence for two new loci by means of a new screening procedure

Dequard-Chablat

1985

Genet. Res.

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I describe here a new screening procedure to isolate ribosomal suppressors in Podospora anserina. I have used the sporulation defect displayed by an antisuppressor mutation AST-2. The revertants able to sporulate are due to either true reversions or external mutations. The mutations which restore most efficiently the sporulation show all the properties of ribosomal suppressors and are localized in two new suppressor loci sull and sul2.

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“…Natural suppression, the readthrough over termination codons by tRNAs from wild-type cells, may be of certain general importance because it would allow an organism or a virus to produce two proteins from one cistron (Picard-Bennoun, 1982;Ryoji et al, 1983). A classic example for natural readthrough are two major viral proteins of the TMV-infected plant cell, which, on the basis of the known TMV sequence (Goelet et al, 1982) are now designated as the 126-K protein and the 183-K readthrough protein.…”

Section: Discussionmentioning

confidence: 99%

UAG readthrough during TMV RNA translation: isolation and sequence of two tRNAs^Tyr with suppressor activity from tobacco plants

et al. 1984

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The hypothetical replicase or replicase subunit cistron in the 5′‐proximal part of tobacco mosaic virus (TMV) RNA yields a major 126‐K protein and a minor 183‐K ‘readthrough’ protein in vivo and in vitro. Two natural suppressor tRNAs were purified from uninfected tobacco plants on the basis of their ability to promote readthrough over the corresponding UAG termination codon in vitro. In a reticulocyte lysate the yield of 183‐K readthrough protein increases from ˜10% in the absence of added tobacco plant tRNA up to ˜35% in the case of pure tRNATyr added. Their amino acid acceptance and anticodon sequence (GψA) identifies the two natural suppressor tRNAs as the two normal major cytoplasmic tyrosine‐specific tRNAs. tRNATyr1 has an A:U pair at the base of the TψC stem and an unmodified G10, whereas tRNATyr2 contains a G:C pair in the corresponding location and m2G in position 10. This is the first case that, in a higher eukaryote, the complete structure is known of both the natural suppressor tRNAs and the corresponding viral RNA on which they exert their function. The corresponding codon‐anticodon interaction, which is not in accordance with the wobble hypothesis, and the possible biological significance of the readthrough phenomenon is discussed.

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Does translational ambiguity increase during cell differentiation?

Cited by 27 publications

References 29 publications

Peptidyl‐tRNA hydrolase in Bacillus subtilis, encoded by spoVC, is essential to vegetative growth, whereas the homologous enzyme in Saccharomyces cerevisiae is dispensable

Peptidyl‐tRNA hydrolase in Bacillus subtilis, encoded by spoVC, is essential to vegetative growth, whereas the homologous enzyme in Saccharomyces cerevisiae is dispensable

Ribosomal suppressors inPodospora anserina: evidence for two new loci by means of a new screening procedure

UAG readthrough during TMV RNA translation: isolation and sequence of two tRNAs^Tyr with suppressor activity from tobacco plants

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Does translational ambiguity increase during cell differentiation?

Cited by 27 publications

References 29 publications

Peptidyl‐tRNA hydrolase in Bacillus subtilis, encoded by spoVC, is essential to vegetative growth, whereas the homologous enzyme in Saccharomyces cerevisiae is dispensable

Peptidyl‐tRNA hydrolase in Bacillus subtilis, encoded by spoVC, is essential to vegetative growth, whereas the homologous enzyme in Saccharomyces cerevisiae is dispensable

Ribosomal suppressors inPodospora anserina: evidence for two new loci by means of a new screening procedure

UAG readthrough during TMV RNA translation: isolation and sequence of two tRNAsTyr with suppressor activity from tobacco plants

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UAG readthrough during TMV RNA translation: isolation and sequence of two tRNAs^Tyr with suppressor activity from tobacco plants