The transition period in dairy cows is generally accepted as the period covering 3 weeks before calving and 3 weeks after calving. The aim of the study was to compare the profiles of β-hydroxybutyrate, calcium, magnesium, phosphorus, total cholesterol, total protein, triacylglycerol, free glycerol, serum lipid and serum protein with Anti-Mullerian Hormone (AMH) in dairy cows in the transition period, and to determine whether AMH to examine whether it can be used as a marker in the next insemination period. The cows whose blood samples were taken were followed up and it was determined that they became pregnant at the insemination and the study was terminated. According to the results obtained; It was determined that BHB and free glycerol, which are important markers of negative energy balance (NEB), have an effect on AMH concentration. However, it was determined that the concentration of magnesium and the ratio of cholesterol ester in serum total fat did not change much during the transition period. Our results suggest that AMH is a good biomarker of decreased follicular activity due to NEB in the transition period and that AMH can be used for herd weeding in reinsemination.