Dog’s genotype of Giardia duodenalis in human: first evidence in Europe

Štrkolcová, Gabriela; Maďar, Marián; Hinney, Barbara; Goldová, M.; Mojžišová, Jana; Halánová, Monika

doi:10.1515/ap-2015-0113

Cited by 45 publications

(19 citation statements)

References 15 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The remaining six assemblages (C to H) are host-specific (Feng and Xiao, 2011;Ryan and Caccio, 2013;Santin et al, 2012). However, assemblages C, D, E, and F have been reported from human at lower frequency in sporadic cases (Feng and Xiao, 2011;Liu et al, 2014;Strkolcova et al, 2015).…”

Section: Introductionmentioning

confidence: 98%

Multilocus genotyping of Giardia duodenalis isolates from calves in Oromia Special Zone, Central Ethiopia

Wegayehu

Karim

Erko

et al. 2016

Infection, Genetics and Evolution

View full text Add to dashboard Cite

Section: Introductionmentioning

confidence: 98%

Multilocus genotyping of Giardia duodenalis isolates from calves in Oromia Special Zone, Central Ethiopia

Wegayehu

Karim

Erko

et al. 2016

Infection, Genetics and Evolution

View full text Add to dashboard Cite

“…The remaining six assemblages (C to H) are host-specific (specific to animals). However, assemblages C, D, E, and F have been reported to cause human giardiasis in rare cases [ 3 , 9 , 10 ].…”

Section: Introductionmentioning

confidence: 99%

Multilocus genotyping of Giardia duodenalis isolates from children in Oromia Special Zone, central Ethiopia

Wegayehu

Karim

et al. 2016

BMC Microbiol

View full text Add to dashboard Cite

BackgroundGiardia duodenalis is the etiologic agent of giardiasis in humans and other mammals worldwide. The burden of disease is high among children in developing countries where sanitation is inadequate. However, the epidemiology and genetic diversity of this parasite is poorly understood in Ethiopia. This study aimed to determine the prevalence and genetic diversity of G. duodenalis in asymptomatic children in Oromia Special Zone, central Ethiopia.ResultsA total of 286 fresh fecal specimens were collected from children and screened using microscopy and PCR. The prevalence of Giardia duodenalis was 10.8 % (31/286) and 16.8 % (48/286) as detected by microscopy and nested PCR, respectively. The infection rate by the study area, sex and age group difference was not significant (P > 0.05). Genotyping results showed that 22.9 % (11/48) of the isolates belonged to assemblage A while 77.1 % (37/48) belonged to assemblage B. Although double peaks were observed at the chromatogram level, no mixed assemblage or sub-assemblage infections were demonstrated. Isolates of assemblage A mostly belonged to the sub-assemblage AII and showed similarity with previously described isolates. However, there was great genetic variability within assemblage B that showed heterogeneous nucleotide positions. Fifteen of them were new genotypes: 5 at the triose phosphate isomerase (tpi), 2 at the β-giardin (bg), and 8 at the glutamate dehydrogenase (gdh) genes.ConclusionsGiardia duodenalis mainly assemblage B infection was predominant among the asymptomatic children in the study area. The high polymorphism found in isolates of assemblage B warrants a more defining tool to discriminate assemblage B at the sub-assemblage level. The findings of the present study indicate that there is a need to carry out national screening programs aiming to detect asymptomatic infections to minimize the reservoir of the disease.Electronic supplementary materialThe online version of this article (doi:10.1186/s12866-016-0706-7) contains supplementary material, which is available to authorized users.

show abstract

“…cysts only in dogs less than 1 year, the percentage reached to 1.9%. Zoonotic potential of Giardia is limited, but con rmed transmission of speci c assemblage C from dog to human indicates a risk for immunode ciency patients [11]. Using molecular biological methods we have focused on dangerous zoonotic tapeworms.…”

Section: Discussionmentioning

confidence: 99%

“…German research study has shown that dogs predominantly host speci c Giardia assemblages C and D, but zoonotic assemblage A has also been proved in dogs [10]. Con rmed transmission of speci c assemblage C from dog to human pointed out to the immunode ciency in patients as an important risk factor [11].…”

Section: Introductionmentioning

confidence: 99%

Factors Affecting the Occurrence of Gastrointestinal Parasites and Lungworms in Dogs and Assessment of Antiparasitic Drugs use Patterns

Vernerová¹,

Dvorakova²,

Svobodová³

et al. 2020

Preprint

View full text Add to dashboard Cite

Background Endoparasites of the canine gastrointestinal and respiratory tract represent a wide range of protozoans and helminths. They are causative agents of different serious diseases of animals and many of them also pose zoonotic risks to human health. The education of dog owners and their families with the principles of human and animal health protection may especially prevent the spread of zoonoses such giardiosis, toxocarosis, echinococcosis and Taenia crassiceps infection. Pulmonary nematods are not zoonotic but they can cause serious and potentially fatal disease in dogs.MethodsThree hundred ninety-one samples of canine faeces and 398 completed questionnaires were collected. The questionnaires comprised of description of individual dogs and detailed data about the way the dogs were kept and their deworming. Samples were examined by flotation, larvoscopy by Baermann and PCR method.ResultsWe confirmed infection of Giardia spp., Isospora ohioensis, Neospora caninum/Hammondia heydorni, Ancylostoma spp., Angiostrongylus vasorum, Capillaria spp., Crenosoma vulpis, Toxocara canis, Toxascaris leonina, Trichuris spp. and Taenia spp. Sequences were obtained for 3 of them confirming T. crassiceps. No deworming treatment was recorded for insignificant number of dogs. Dog owners most often used multicomponent endoparasitics.ConclusionsMost of the dogs were dewormed irregularly and antiparasitics were administered namely twice a year, so monthly intervals were not performed in the group of dogs at risk for e.g. possibility of predation. Consumption of antiparasitic drugs cannot ensure the protection of dogs, their owners and other people, unless not applied purposefully, with appropriate knowledge and on the basis of the veterinarian's advice.

show abstract

Dog’s genotype of Giardia duodenalis in human: first evidence in Europe

Cited by 45 publications

References 15 publications

Multilocus genotyping of Giardia duodenalis isolates from calves in Oromia Special Zone, Central Ethiopia

Multilocus genotyping of Giardia duodenalis isolates from calves in Oromia Special Zone, Central Ethiopia

Multilocus genotyping of Giardia duodenalis isolates from children in Oromia Special Zone, central Ethiopia

Factors Affecting the Occurrence of Gastrointestinal Parasites and Lungworms in Dogs and Assessment of Antiparasitic Drugs use Patterns

Contact Info

Product

Resources

About