Due to declining iron ore grades, low-grade ores with complex mineralogy, namely, banded iron formation (BIF), are being explored to maximize iron ore resources and increase the life of mine. Flotation is the standard global process used for the beneficiation of BIF ores, as it is a complex ore reserve, and liberation is typically achieved below 1 mm. This study focuses on flotation of a South African BIF evaluating its response to different collectors, as well as effect of grind. Since flotation is an expensive process and South Africa ranks high on the production cost curve, pre-concentration via gravity or magnetic separation is performed prior to flotation. Thus, flotation was also performed on a gravity concentrate to determine if a grade suitable for steelmaking (~ 63%) is achievable. The results indicate that the use of an alkyl ether amine-based collector partially neutralized with acetic acid is suitable for separation of hematite from illite and quartz at high pH values. Further optimization tests are being pursued to improve overall recovery and grade.