Dolosigranulum pigrum: Predicting Severity of Infection

Sherret, John; Gajjar, Bhavesh; Ibrahim, Lamis; Ahmed, Ahmed Mohamed; Panta, Utsab R

doi:10.7759/cureus.9770

Cited by 9 publications

(13 citation statements)

References 21 publications

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“…1 ). These findings are consistent with there being only a few isolated reports of D. pigrum growth in samples from different types of infections ( 77 – 82 ). Of these, the repeated detection of D. pigrum alone in keratitis/keratoconjunctivitis raises the possibility that some strains might be rare causes of eye surface infection ( 83 – 86 ).…”

Section: Discussionsupporting

confidence: 92%

Genomic Stability and Genetic Defense Systems in Dolosigranulum pigrum , a Candidate Beneficial Bacterium from the Human Microbiome

Ramos¹,

Brugger

Escapa

et al. 2021

mSystems

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Section: Discussionsupporting

confidence: 92%

Genomic Stability and Genetic Defense Systems in Dolosigranulum pigrum , a Candidate Beneficial Bacterium from the Human Microbiome

Ramos¹,

Brugger

Escapa

et al. 2021

mSystems

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“…1). These findings are consistent with there being only a few isolated reports of D. pigrum growth in samples from different types of infections (77)(78)(79)(80)(81)(82). Of these, the repeated detection of D.…”

Section: Discussionsupporting

confidence: 91%

Genomic Stability and Genetic Defense Systems inDolosigranulum pigruma Candidate Beneficial Bacterium from the Human Microbiome

Ramos

Brugger

Escapa³

et al. 2021

Preprint

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Dolosigranulum pigrum is positively associated with indicators of health in multiple epidemiological studies of human nasal microbiota. Knowledge of the basic biology of D. pigrum is a prerequisite for evaluating its potential for future therapeutic use; however, such data are very limited. To gain insight into D. pigrum's chromosomal structure, pangenome and genomic stability, we compared the genomes of 28 D. pigrum strains that were collected across 20 years. Phylogenomic analysis showed closely related strains circulating over this period and closure of 19 genomes revealed highly conserved chromosomal synteny. Gene clusters involved in the mobilome and in defense against mobile genetic elements (MGEs) were enriched in the accessory genome versus the core genome. A systematic analysis for MGEs identified the first candidate D. pigrum prophage and insertion sequence. A systematic analysis for genetic elements that limit the spread of MGEs, including restriction modification (RM), CRISPR-Cas, and deity-named defense systems, revealed strain-level diversity in host defense systems that localized to specific genomic sites including one RM system hotspot. Analysis of CRISPR spacers pointed to a wealth of MGEs against which D. pigrum defends itself. These results reveal a role for horizontal gene transfer and mobile genetic elements in strain diversification while highlighting that in D. pigrum this occurs within the context of a highly stable chromosomal organization protected by a variety of defense mechanisms.

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“…Of the 10 dominant genera in the nasal bacteriome ( Table 1 ), 6 formed subnetworks in CT, 8 in AR and 10 in ARAS. Interestingly, Moraxella and Staphylococcus only appeared in the networks of rhinitic patients alone or connected to the opportunistic pathogen Dolosigranulum pigrum [ 94 ]. Similarly, other commensal genera (e.g., Corynebacterium and Veillonella ) were also associated in separate modules, suggesting a robust relationship.…”

Section: Resultsmentioning

confidence: 99%

Nasal Bacteriomes of Patients with Asthma and Allergic Rhinitis Show Unique Composition, Structure, Function and Interactions

et al. 2023

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Allergic rhinitis and asthma are major public health concerns and economic burdens worldwide. However, little is known about nasal bacteriome dysbiosis during allergic rhinitis, alone or associated with asthma comorbidity. To address this knowledge gap we applied 16S rRNA high-throughput sequencing to 347 nasal samples from participants with asthma (AS = 12), allergic rhinitis (AR = 53), allergic rhinitis with asthma (ARAS = 183) and healthy controls (CT = 99). One to three of the most abundant phyla, and five to seven of the dominant genera differed significantly (p < 0.021) between AS, AR or ARAS and CT groups. All alpha-diversity indices of microbial richness and evenness changed significantly (p < 0.01) between AR or ARAS and CT, while all beta-diversity indices of microbial structure differed significantly (p < 0.011) between each of the respiratory disease groups and controls. Bacteriomes of rhinitic and healthy participants showed 72 differentially expressed (p < 0.05) metabolic pathways each related mainly to degradation and biosynthesis processes. A network analysis of the AR and ARAS bacteriomes depicted more complex webs of interactions among their members than among those of healthy controls. This study demonstrates that the nose harbors distinct bacteriotas during health and respiratory disease and identifies potential taxonomic and functional biomarkers for diagnostics and therapeutics in asthma and rhinitis.

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Dolosigranulum pigrum: Predicting Severity of Infection

Cited by 9 publications

References 21 publications

Genomic Stability and Genetic Defense Systems in Dolosigranulum pigrum , a Candidate Beneficial Bacterium from the Human Microbiome

Genomic Stability and Genetic Defense Systems in Dolosigranulum pigrum , a Candidate Beneficial Bacterium from the Human Microbiome

Genomic Stability and Genetic Defense Systems inDolosigranulum pigruma Candidate Beneficial Bacterium from the Human Microbiome

Nasal Bacteriomes of Patients with Asthma and Allergic Rhinitis Show Unique Composition, Structure, Function and Interactions

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