Domain fusion between SNF1‐related kinase subunits during plant evolution

Lumbreras, Victoria; Albà, M. Mar; Kleinow, Tatjana; Koncz, Csaba; Pagès, Montserrat

doi:10.1093/embo-reports/kve001

Cited by 81 publications

(97 citation statements)

References 16 publications

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“…It has been proposed that plants may possess two distinct types of SNF1 complexes with two or three subunits (Lumbreras et al, 2001). In line with our observation, the bg-subunit Os03g63940 has been associated with the Os05g45420 kinase in planta by TAPtag analyses (J.S.…”

Section: Proteins Associated With Rice Snrkssupporting

confidence: 90%

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A Rice Kinase-Protein Interaction Map

et al. 2008

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Plants uniquely contain large numbers of protein kinases, and for the vast majority of the 1,429 kinases predicted in the rice (Oryza sativa) genome, little is known of their functions. Genetic approaches often fail to produce observable phenotypes; thus, new strategies are needed to delineate kinase function. We previously developed a cost-effective high-throughput yeast twohybrid system. Using this system, we have generated a protein interaction map of 116 representative rice kinases and 254 of their interacting proteins. Overall, the resulting interaction map supports a large number of known or predicted kinaseprotein interactions from both plants and animals and reveals many new functional insights. Notably, we found a potential widespread role for E3 ubiquitin ligases in pathogen defense signaling mediated by receptor-like kinases, particularly by the kinases that may have evolved from recently expanded kinase subfamilies in rice. We anticipate that the data provided here will serve as a foundation for targeted functional studies in rice and other plants. The application of yeast two-hybrid and TAPtag analyses for large-scale plant protein interaction studies is also discussed.

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Section: Proteins Associated With Rice Snrkssupporting

confidence: 90%

“…4; Lumbreras et al, 2001). This class of proteins is characterized by the presence of an N-terminal KIS domain (characteristic of b-subunits) and a C-terminal region related to the animal SNF4/AMPKg protein (Supplemental Fig.…”

Section: Proteins Associated With Rice Snrksmentioning

confidence: 99%

A Rice Kinase-Protein Interaction Map

et al. 2008

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“…Chimeric genes have been identified and characterized from many plant species (Lumbreras et al 2001;Hedgcoth et al 2002) and mammals (Arguello et al 2006). Recently, various chimeric genes have been isolated from wheat storage protein loci, including the LMW-GS/gliadin chimeric gene from an old Hungarian variety (Nagy et al 2005) and LMW-GS from wild wheat relatives .…”

Section: Maldi-tof-ms Analyses Confirmed That 1dx5mentioning

confidence: 99%

Molecular cloning of a novel chimeric HMW glutenin subunit gene 1Dx5′ from a common wheat line W958

Feng

et al. 2010

Mol Breeding

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A novel chimeric high-molecular-weight (HMW) glutenin subunit gene from a new common wheat line W958 (2n = 6x = 42) was isolated and characterized. SDS-PAGE analysis revealed that this glutenin subunit has similar electrophoretic mobility to 1Dx5, so it was designated 1Dx5 0 . Genomic DNA from W958 was amplified and a 2,505-bp fragment was obtained. The 1Dx5 0 subunit showed a chimeric primary structure of 1Dx5 and 1Dx2, with the 1Dx5 sequence in the 5 0 and middle repetitive regions and the 1Dx2 sequence in the repetitive domain and 3 0 region. MALDI-TOF-MS analysis demonstrated that 1Dx5 0 had a molecular weight of 86815.1 Da, close to that of an x-type glutenin subunit. Secondary structure analysis showed that this subunit had six helixes and one strand, including four helixes in the repetitive domain which could enhance the dough properties. Additionally, the promoter of 1Dx5 0 was obtained and showed the same sequence as 1Dx5 or 1Dx2 except for a few base conversions. The promoter analysis indicated that the cis-acting regulatory elements of 1Dx5 0 were the same as those of 1Dx5 and/or 1Dx2. Previously, we have demonstrated that this novel glutenin subunit is associated with good bread-making quality and comprises a very large proportion of the F2 segregation population.Electronic supplementary material The online version of this article

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“…). In addition, numerous chimeric genes were separated and characterized from various plant species (Lumbreras et al 2001;Hedgcoth et al 2002) and from bacteria, yeast, Drosophila, and mammals (Roth and Wilson 1986;Allgood and Silhavy 1988;Arguello et al 2006). It was obvious that the chimeric genes were generated by recombination and crossing over among different genes and coding loci as suggested by Nagy et al (2005).…”

Section: Pcr Amplification and Cloning Of Lmw-gs Genesmentioning

confidence: 99%

A Novel Chimeric Low-Molecular-Weight Glutenin Subunit Gene From the Wild Relatives of Wheat Aegilops kotschyi and Ae. juvenalis: Evolution at the Glu-3 Loci

Gao

et al. 2008

Genetics

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Four LMW-m and one novel chimeric (between LMW-i and LMW-m types) low-molecular-weight glutenin subunit (LMW-GS) genes from Aegilops neglecta (UUMM), Ae. kotschyi (UUSS), and Ae. juvenalis (DDMMUU) were isolated and characterized. Sequence structures showed that the 4 LMW-m-type genes, assigned to the M genome of Ae. neglecta, displayed a high homology with those from hexaploid common wheat. The novel chimeric gene, designed as AjkLMW-i, was isolated from both Ae. kotschyi and Ae. juvenalis and shown to be located on the U genome. Phylogentic analysis demonstrated that it had higher identity to the LMW-m-type than the LMW-i-type genes. A total of 20 single nucleotide polymorphisms (SNPs) were detected among the 4 LMW-m genes, with 13 of these being nonsynonymous SNPs that resulted in amino acid substitutions in the deduced mature proteins. Phylogenetic analysis demonstrated that it had higher identity to the LMW-m-type than the LMW-i-type genes. The divergence time estimation showed that the M and D genomes were closely related and diverged at 5.42 million years ago (MYA) while the differentiation between the U and A genomes was 6.82 MYA. We propose that, in addition to homologous recombination, an illegitimate recombination event on the U genome may have occurred 6.38 MYA and resulted in the generation of the chimeric gene AjkLMW-i, which may be an important genetic mechanism for the origin and evolution of LMW-GS Glu-3 alleles as well as other prolamin genes.

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Domain fusion between SNF1‐related kinase subunits during plant evolution

Cited by 81 publications

References 16 publications

A Rice Kinase-Protein Interaction Map

A Rice Kinase-Protein Interaction Map

Molecular cloning of a novel chimeric HMW glutenin subunit gene 1Dx5′ from a common wheat line W958

A Novel Chimeric Low-Molecular-Weight Glutenin Subunit Gene From the Wild Relatives of Wheat Aegilops kotschyi and Ae. juvenalis: Evolution at the Glu-3 Loci

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