The half-metallic CrO 2 with nearly 100% spin polarization is an ideal system to study magnetic domain wall resistance, which differs from the resistance (or resistivity) inside a single-domain. To experimentally measure the domain wall resistance, we design and prepare a special CrO 2 epitaxial nanostructure with an asymmetrical weak link to localize a single domain wall, by using the techniques of chemical vapor deposition and selective-area growth. This structure provides a capability to generate and annihilate a domain wall near the weak link. By contrasting the resistance between a single-domain state and a domain state, we observe a repeatable and reversible resistance jump, namely domain wall resistance, in half metallic CrO 2. Using the Levy-Zhang model, we further obtain the spin asymmetry ratio / between resistivities in the two spin channels. The ratio, 4256 ± 388 at 5.0 K, is much larger than that of conventional ferromagnetic metals, attesting the half-metallicity of CrO 2 .