Dome lighting for insect imaging under a microscope

Kerr, Peter H.; Fisher, E.; Buffington, Matthew L.

doi:10.1093/ae/54.4.198

Cited by 87 publications

(64 citation statements)

References 1 publication

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“…Cartograph 5.6.0 was used to merge an image series into a single in-focus image. Lighting was achieved using techniques summarised in Buffington et al (2005), Buffington and Gates (2009) and Kerr et al (2009). Images included in this paper are available on WaspWeb (http:// www.waspweb.org), and are archived at Morphbank (http:// www.morphbank.net) and in Specimage (http://specimage.osu.…”

Section: Field Collections Observations and Imagingmentioning

confidence: 99%

Systematics and biology of the aberrant intertidal parasitoid wasp Echthrodesis lamorali Masner (Hymenoptera : Platygastridae s.l.): a parasitoid of spider eggs

Noort

Masner

Popovici

et al. 2014

Invert. Systematics

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Abstract. The platygastroid wasp Echthrodesis lamorali has been of considerable interest since its description in 1968, primarily because of its highly modified, densely pilose, wingless body, its distribution and unusual biology. The species is endemic to the Cape Peninsula, South Africa, where it is an endoparasitoid of eggs of the marine spiders Desis formidabilis (Desidae) and Amaurobioides africanus (Anyphaenidae) in the intertidal region. Although a highly aberrant monospecific genus, the phylogenetic relationships of Echthrodesis are confused, in part due to convergence in body form across numerous unrelated platygastroid genera. We used sequence data from the nuclear 28S rRNA and 18S rDNA genes, and the mitochondrial cytochrome oxidase 1 (CO1) gene, to determine the phylogenetic affinities of E. lamorali. We present a revised taxonomic description for the genus and species, as well as new morphological information on the structure of its mouthparts and ovipositor system. Phylogenetic analyses of molecular data place E. lamorali within one of two independent clades of platygastroid wasps that use spider eggs as hosts. Echthrodesis is sister to a group of three genera: Neobaeus (New Zealand; host unconfirmed); Mirobaeoides (Australia; spider eggs); and Embidobia (near cosmopolitan; embiid eggs). Details on the biology, behaviour and morphological adaptations of E. lamorali are provided.

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Section: Field Collections Observations and Imagingmentioning

confidence: 99%

Systematics and biology of the aberrant intertidal parasitoid wasp Echthrodesis lamorali Masner (Hymenoptera : Platygastridae s.l.): a parasitoid of spider eggs

Noort

Masner

Popovici

et al. 2014

Invert. Systematics

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show abstract

“…To eliminate reflections from the metallic and shiny body, a dome light manufactured as described by Kerr et al (2008), was used as the light source for photography. Photos were taken at different focus levels and Helicon Focus Pro version 4.75 was used to merge them into a single image.…”

Section: Methodsmentioning

confidence: 99%

Pediobius cajanus sp. n. (Hymenoptera, Eulophidae), an important natural enemy of the Asian fly (Melanagromyza obtusa (Malloch)) (Diptera, Agromyzidae) in the Dominican Republic

Taverasl¹,

Hansson

2015

JHR

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Pediobius cajanus sp. n. is described based on material from the Dominican Republic, where it is widespread, and it is anticipated to have a much larger distribution in tropical America. It is compared to other species of Pediobius from the New World, and is also compared to P. vignae (Risbec), a similar species from Africa with similar biology. The new species is a gregarious endoparasitoid of the pupae of the Asian fly, Melanagromyza obtusa (Malloch), an agromyzid that causes major damage to pigeon pea, Cajanus cajan (L). Millspauh. In the Dominican Republic P. cajanus sp. n. is the most important parasitoid of this pest. Details on its biology are provided.

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“…To eliminate reflections from the metallic and shiny body, a domelight manufactured as described by Kerr et al (2008), was used as the light source for photography. Photos were taken at different focus levels and Helicon Focus Pro version 4.75 was used to merge them into a single image.…”

Section: Methodsmentioning

confidence: 99%

Three cryptic species in Asecodes (Förster) (Hymenoptera, Eulophidae) parasitizing larvae of Galerucella spp. (Coleoptera, Chrysomelidae), including a new species

Hansson¹,

Hambäck²

2013

JHR

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Three morphologically very similar species of Asecodes Förster (Hymenoptera: Eulophidae) are reviewed. Asecodes parviclava (Thomson) is removed from synonymy under A. lucens stat. rev., and differentiated from A. lucens (Nees) and A. lineophagum sp. n. All three species develop as gregarious endoparasitoids in larvae of Galerucella spp. (Coleoptera: Chrysomelidae), but each species has its own unique host range. Asecodes lineophagum attacks only Galerucella lineola (Fabr.) and A. lucens only G. sagittariae (Gyllenhal), whereas A. parviclava parasitizes G. tenella (L.), G. calmariensis (L.) and G. pusilla (Duftschmid). The Asecodes species are similar but display small though distinct morphological differences, and are distinguished also through molecular differences. The genetic distance in mitochondrial CO1 ranged from 2.3% to 7.3% between the species. Five names, one valid and four synonyms, were available for this group of species, but none of them was linked to a primary type. To promote stability of nomenclature, primary types are designated for all five names, neotypes for Eulophus lucens Nees, Entedon mento Walker and Derostenus parviclava Thomson, and lectotypes for Entedon chthonia Walker and Entedon metagenes Walker. Entedon mento, E. chthonia and E. metagenes remain synonymized under A. lucens.

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Dome lighting for insect imaging under a microscope

Cited by 87 publications

References 1 publication

Systematics and biology of the aberrant intertidal parasitoid wasp Echthrodesis lamorali Masner (Hymenoptera : Platygastridae s.l.): a parasitoid of spider eggs

Systematics and biology of the aberrant intertidal parasitoid wasp Echthrodesis lamorali Masner (Hymenoptera : Platygastridae s.l.): a parasitoid of spider eggs

Pediobius cajanus sp. n. (Hymenoptera, Eulophidae), an important natural enemy of the Asian fly (Melanagromyza obtusa (Malloch)) (Diptera, Agromyzidae) in the Dominican Republic

Three cryptic species in Asecodes (Förster) (Hymenoptera, Eulophidae) parasitizing larvae of Galerucella spp. (Coleoptera, Chrysomelidae), including a new species

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