Microbial fuel cells (MFCs) are regarded as one of the best potential natural resources to be discovered as a way towards reducing the over-dependence on fossil-fuel-based electricity generation. But, Limitations of advanced study on MFCs have slow-downed its development. Moreover, Low power generations from MFCs, expensive electrode materials, and the inability to scale up MFCs to industrially relevant capacities have made that worse. However, the Utilization of MFC in the arena of electro-chemistry and thermal science can be very promising in other aspects. In this work, we studied various materials and processes that can be applied to construct economical MFCs on small scale. Then, a Charge-Discharge cycling experiment was conducted on the Cell. During the test, Voltage generation close to Lithium-Ion Batteries (LIBs) was identified which shows MFCs can be used as batteries. Next, a single spherical particle one-dimensional (1D) physics-based thermo-electrochemical model was developed to investigate the potential overall performance of MFC as an energy storage device. Using our model in simulation, we validated the experimental results to predict the techniques required to analyze the system's optimum performance and provided recommendations on theories and parameters to include in MFC studies to achieve the best possible outcomes. The outcome of this work will provide useful information on the concept of MFC and design guidelines for various applications.