Domestication and growth hormone transgenesis cause similar changes in gene expression in coho salmon ( Oncorhynchus kisutch )

Abstract

Domestication has been extensively used in agricultural animals to modify phenotypes such as growth rate. More recently, transgenesis of growth factor genes [primarily growth hormone (GH)] has also been explored as a rapid approach to accelerating performance of agricultural species. Growth rates of many fishes respond dramatically to GH gene transgenesis, whereas genetic engineering of domestic mammalian livestock has resulted in relatively modest gains. The most dramatic effects of GH transgenesis in fish have been seen in relatively wild strains that have undergone little or no selection for enhanced growth, whereas genetic modification of livestock necessarily has been performed in highly domesticated strains that already possess very rapid growth. Such fast-growing domesticates may be refractory to further stimulation if the same regulatory pathways are being exploited by both genetic approaches. By directly comparing gene expression in wild-type, domestic, and GH transgenic strains of coho salmon, we have found that domestication and GH transgenesis are modifying similar genetic pathways. Genes in many different physiological pathways show modified expression in domestic and GH transgenic strains relative to wild-type, but effects are strongly correlated. Genes specifically involved in growth regulation (IGF1, GHR, IGF-II, THR) are also concordantly regulated in domestic and transgenic fish, and both strains show elevated levels of circulating IGF1. Muscle expression of GH in nontransgenic strains was found to be elevated in domesticated fish relative to wild type, providing a possible mechanism for growth enhancement. These data have implications for genetic improvement of existing domesticated species and risk assessment and regulation of emerging transgenic strains.

A novel GH secretagogue, A233, exhibits enhanced growth activity and innate immune system stimulation in teleosts fish

“…In addition to its essential role in the regulation of body growth and development, it can also influence reproduction, immunity, osmoregulation, and behavior (Rise et al 2006, Devlin et al 2009). In teleosts, secretion of GH is regulated by several hypothalamic factors that are influenced by the physiological state of the animal.…”

Fast Transcriptional Responses to Domestication in the Brook CharrSalvelinus fontinalis

“…They concluded that the differences observed were of genetic origin and that these were either the direct or indirect result of artificial selection. Finally, Devlin et al (2008) showed in coho salmon (Oncorhynchus kisutch) that domestication and growth hormone transgenesis cause similar changes in expression for genes involved in energy metabolism of carbohydrates and lipids, protein synthesis, stress and immune functions, and cellular structure.…”

Transmisi Dan Ekspresi Fenotipe Gen Penyandi Hormon Pertumbuhan Pada Ikan Patin Siam

“…Beberapa faktor ini, diduga menyebabkan beberapa individu yang disisipi gen penyandi hormon pertumbuhan berukuran lebih besar dibandingkan kontrol (Devlin et al, 2009). …”